Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (3): 328-334.DOI: 10.11983/CBB18117
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Guangqian Cheng1,†,Keli Jia1,2,†,Na Li1,Chuanliang Deng1,Shufen Li1,Wujun Gao1,*
Received:
2018-05-11
Accepted:
2018-08-06
Online:
2019-07-01
Published:
2019-11-24
Contact:
Wujun Gao
Guangqian Cheng,Keli Jia,Na Li,Chuanliang Deng,Shufen Li,Wujun Gao. Bioinformatics Analysis and Chromosome Location of Nuclear Integrants of Plastid DNA in Asparagus officinalis[J]. Chinese Bulletin of Botany, 2019, 54(3): 328-334.
IR | LSC | SSC | |
---|---|---|---|
Total sequence length (bp) | 26471 | 83821 | 17904 |
Number of NUPTs | 726 | 1227 | 286 |
Average density (No./kb) | 27.9 | 14.6 | 15.9 |
Average length of NUPTs (bp) | 241 | 258 | 246 |
The length of NUPTs (bp) | 175215 | 316626 | 70394 |
Table 1 Origin analysis of nuclear integrants of plastid DNAs (NUPTs) sequences on the chloroplast genome of Asparagus officinalis
IR | LSC | SSC | |
---|---|---|---|
Total sequence length (bp) | 26471 | 83821 | 17904 |
Number of NUPTs | 726 | 1227 | 286 |
Average density (No./kb) | 27.9 | 14.6 | 15.9 |
Average length of NUPTs (bp) | 241 | 258 | 246 |
The length of NUPTs (bp) | 175215 | 316626 | 70394 |
Figure 3 PCR amplification of inverted repeat region (IR) two sequences of region in Asparagus officinalis 1, 2 respresents the amplification results of AocplR1 and AocplR2, respectively.
Figure 4 Mitosis metaphase chromosome locations of AocpIR1 and AocpIR2 of Asparagus officinalis (A), (E) FISH result of 45S rDNA; (B), (F) FISH result of AocpIR1 and AocpIR2; (C), (G) Merged picture; (D), (H) Karyotype analysis. Bars=10 μm
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