Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (3): 343-349.DOI: 10.11983/CBB18106
• EXPERIMENTAL COMMUNICATIONS • Previous Articles Next Articles
Xia Zhang1,Xiang Jing1,Guangcai Zhou2,Ying Bao1,*()
Received:
2018-04-26
Accepted:
2018-12-10
Online:
2019-07-01
Published:
2019-11-24
Contact:
Ying Bao
Xia Zhang,Xiang Jing,Guangcai Zhou,Ying Bao. Phylogeny and Tissue-specific Expression of the GBSS Genes in Oryza officinalis[J]. Chinese Bulletin of Botany, 2019, 54(3): 343-349.
Gene | Primer name | Primer sequence (5'-3') |
---|---|---|
GBSSI | GBSSI-F | AACGTGGCTGCTCCTTGAA |
GBSSI-R | TTGGCAATAAGCCACACACA | |
GBSSII | GBSSII-F | AGGCATCGAGGGTGAGGAG |
GBSSII-R | CCATCTGGCCCACATCTCTA |
Table 1 Primer sequences of GBSSI and GBSSII
Gene | Primer name | Primer sequence (5'-3') |
---|---|---|
GBSSI | GBSSI-F | AACGTGGCTGCTCCTTGAA |
GBSSI-R | TTGGCAATAAGCCACACACA | |
GBSSII | GBSSII-F | AGGCATCGAGGGTGAGGAG |
GBSSII-R | CCATCTGGCCCACATCTCTA |
Plants | Gene (GenBank/UniProt No.) | Sequence identity (%) | |
---|---|---|---|
Oryza officinalis | |||
GBSSI | GBSSII | ||
Oryza sativa subsp. japonica | GBSSI (XP_025882300.1) | 95.22 | 60.35 |
GBSSII (XP_015647210.1) | 62.30 | 96.72 | |
O. sativa subsp. indica | GBSSI (AAN77100.1) | 97.87 | 62.30 |
GBSSII (ACY56079.1) | 62.46 | 97.04 | |
Leersia perrieri | GBSSI (A0A0D9WLF6) * | 93.01 | 62.15 |
GBSSII (A0A0D9WED4) * | 58.44 | 85.62 | |
Zizania latifolia | GBSSI (ASSH01051725.1) ** | 90.80 | 62.01 |
GBSSII (ASSH01023543.1) ** | 59.46 | 87.47 | |
Brachypodium distachyon | GBSSI (XP_003557139.1) | 79.31 | 61.16 |
GBSSII (XP_003569238.1) | 60.33 | 85.43 | |
Hordeum vulgare | GBSSI (BAC41202.1) | 81.97 | 60.76 |
GBSSII (BAJ99426.1) | 61.85 | 86.12 | |
Setaria italica | GBSSI (AGW27658.1) | 86.72 | 61.90 |
GBSSII (XP_004956034.1) | 62.40 | 87.34 | |
Sorghum bicolor | GBSSI (XP_002436418.1) | 82.52 | 61.90 |
GBSSII (XP_002461889.1) | 62.62 | 86.18 | |
Triticum aestivum | GBSSI (XP_020146905.1) | 81.37 | 60.36 |
GBSSII (AAG27624.1) | 61.79 | 86.09 | |
Zea mays | GBSSI (NP_001105001) | 82.08 | 62.13 |
GBSSII (NP_001334833) | 62.36 | 85.88 | |
Musa acuminata | GBSS1 (KF512020.1) | 66.39 | 65.35 |
GBSS2 (KF512021.1) | 67.96 | 63.99 | |
GBSS3 (KF512023.1) | 63.93 | 62.91 | |
Arabidopsis thaliana | GBSS (XP_020866584.1) | 62.48 | 63.88 |
Gossypium raimondii | GBSS1 (XP_012474755.1) | 63.89 | 66.01 |
GBSS2 (XP_012439861.1) | 63.11 | 66.77 | |
GBSS3 (XP_012486622.1) | 62.75 | 64.01 | |
Solanum lycopersicum | GBSS (NP_001311457.1) | 67.05 | 62.03 |
Carica papaya | GBSS (XP_021900468.1) | 65.91 | 66.45 |
S. tuberosum | GBSS (XP_006343763.1) | 62.89 | 67.27 |
Vitis vinifera | GBSS1 (XP_010660257.1) | 65.52 | 66.23 |
GBSS2 (XP_019081062.1) | 64.07 | 67.76 | |
Amborella trichopoda | GBSS (XP_006837847.1) | 62.81 | 66.17 |
Chlamydomonas reinhardtii | GBSS (XP_001697117.1) | 47.79 | 47.05 |
Table 2 Amino acid sequence identities of GBSSs between Oryza officinalis and other plants
Plants | Gene (GenBank/UniProt No.) | Sequence identity (%) | |
---|---|---|---|
Oryza officinalis | |||
GBSSI | GBSSII | ||
Oryza sativa subsp. japonica | GBSSI (XP_025882300.1) | 95.22 | 60.35 |
GBSSII (XP_015647210.1) | 62.30 | 96.72 | |
O. sativa subsp. indica | GBSSI (AAN77100.1) | 97.87 | 62.30 |
GBSSII (ACY56079.1) | 62.46 | 97.04 | |
Leersia perrieri | GBSSI (A0A0D9WLF6) * | 93.01 | 62.15 |
GBSSII (A0A0D9WED4) * | 58.44 | 85.62 | |
Zizania latifolia | GBSSI (ASSH01051725.1) ** | 90.80 | 62.01 |
GBSSII (ASSH01023543.1) ** | 59.46 | 87.47 | |
Brachypodium distachyon | GBSSI (XP_003557139.1) | 79.31 | 61.16 |
GBSSII (XP_003569238.1) | 60.33 | 85.43 | |
Hordeum vulgare | GBSSI (BAC41202.1) | 81.97 | 60.76 |
GBSSII (BAJ99426.1) | 61.85 | 86.12 | |
Setaria italica | GBSSI (AGW27658.1) | 86.72 | 61.90 |
GBSSII (XP_004956034.1) | 62.40 | 87.34 | |
Sorghum bicolor | GBSSI (XP_002436418.1) | 82.52 | 61.90 |
GBSSII (XP_002461889.1) | 62.62 | 86.18 | |
Triticum aestivum | GBSSI (XP_020146905.1) | 81.37 | 60.36 |
GBSSII (AAG27624.1) | 61.79 | 86.09 | |
Zea mays | GBSSI (NP_001105001) | 82.08 | 62.13 |
GBSSII (NP_001334833) | 62.36 | 85.88 | |
Musa acuminata | GBSS1 (KF512020.1) | 66.39 | 65.35 |
GBSS2 (KF512021.1) | 67.96 | 63.99 | |
GBSS3 (KF512023.1) | 63.93 | 62.91 | |
Arabidopsis thaliana | GBSS (XP_020866584.1) | 62.48 | 63.88 |
Gossypium raimondii | GBSS1 (XP_012474755.1) | 63.89 | 66.01 |
GBSS2 (XP_012439861.1) | 63.11 | 66.77 | |
GBSS3 (XP_012486622.1) | 62.75 | 64.01 | |
Solanum lycopersicum | GBSS (NP_001311457.1) | 67.05 | 62.03 |
Carica papaya | GBSS (XP_021900468.1) | 65.91 | 66.45 |
S. tuberosum | GBSS (XP_006343763.1) | 62.89 | 67.27 |
Vitis vinifera | GBSS1 (XP_010660257.1) | 65.52 | 66.23 |
GBSS2 (XP_019081062.1) | 64.07 | 67.76 | |
Amborella trichopoda | GBSS (XP_006837847.1) | 62.81 | 66.17 |
Chlamydomonas reinhardtii | GBSS (XP_001697117.1) | 47.79 | 47.05 |
Figure 3 Relative expression of GBSSI and GBSSII in leaves and seeds of Oryza officinalis(A), (B) Amplification results of RT-PCR and qRT-PCR for GBSS genes in leaves; (C), (D) Amplification results of RT-PCR and qRT-PCR for GBSS genes in seeds.
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