Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (1): 70-76.DOI: 10.11983/CBB16130

Special Issue: 水稻生物学专辑 (2017年52卷1期)

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Use of SELEX for Research of the DNA Binding Sites of the RFL, a Transcription Factor of Rice

Chengqiang Ding, Zhongyuan Chang, Yan Wang, Shaohua Wang, Yanfeng Ding*   

  1. College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2016-06-13 Accepted:2016-10-13 Online:2017-01-01 Published:2017-01-23
  • Contact: Ding Yanfeng
  • About author:

    # Co-first authors

Abstract: FLORICAULA/LEAFY encodes an evolutionarily conserved land plant-specific transcription factor. It functions by regulating the expression of downstream genes. RFL, the FLORICAULA/LEAFY homolog in rice, controls several important developments in rice: flowering time, plastochron and panicle branching. In this study, we detected the binding specificity of RFL by using the method systematic evolution of ligands by exponential enrichment (SELEX). After seven SELEX processes and sequencing, the binding site of RFL was identified as (C/A)(C/T)NN(T/C/A)G(G/T). The result contributes to clarifying the molecular function of RFL.