Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (1): 61-69.DOI: 10.11983/CBB16126

Special Issue: 水稻生物学专辑 (2017年52卷1期)

• Orginal Article • Previous Articles     Next Articles

Mapping of Pizy6(t), a Gene Conferring Resistance to the Rice Blast Strain LP11, in Oryza sativa subsp. japonica Cultivar Ziyu44

Hu Chaoqin, , Liu Jianyu, , Wang Yunqian, Yang Rui, Wang Bingkun, He Yueqiu, Zeng Qianchun, Luo Qiong*()   

  1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan
    Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming 650201, China
  • Received:2016-06-07 Accepted:2016-07-29 Online:2017-01-01 Published:2017-01-23
  • Contact: Luo Qiong
  • About author:

    # Co-first authors

Abstract: Rice blast, caused by Magnaporthe oryzae infection, is one of the major diseases threatening the sustainable development of rice (Oryza sativa) production worldwide. Between 10% and 30% of the annual rice harvest is lost due to infection by the fungus. Development and breeding utilization of rice-resistant resources are considered the most effective and economic means to control rice blast. Isolating new virulent blast strains from the pathotype Oryza is the basis for cloning novel optimal genes resistant to rice blast and rice breeding for disease resistance. In the present study, we isolated and screened blast strains and identified new genes resistant to rice blast. Overall, 5 strains with significantly different pathogenicity between Ziyu44 and Jiangnanxiangnuo (JNXN) were identified. Eight rice varieties, including Ziyu44, Xiangzi3150, 9311, Nipponbare, Lijiangxintuanheigu (LTH), Zhonghua11, JNXN and TP309, were challenged with the 5 blast strains by spray-inoculation at seedling stage. Xiangzi3150, a variety with broad-spectrum blast resistance, showed obvious attacks after inoculation with LP11, so LP11 is probably a newly emerged strong virulent strain. The F2 progenies derived from the cross between Ziyu44 and JNXN were inoculated with the LP11; genetic analysis and gene mapping indicated that the resistance phenotype of Ziyu44 to LP11 was controlled by a single dominant gene, and the resistance gene Pizy6(t) was located on rice chromosome 6. These results provided valuable blast strains for genetic studies of disease resistance and laid the foundation for Pizy6(t) gene cloning in Ziyu44.