Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (3): 363-368.DOI: 10.11983/CBB15102

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Induction of Hairy Roots of Dianthus chinensis and Its Plant Regeneration

Heping Shi*, Bei Wang, Shunan Yang, Yapeng Guo   

  1. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China
  • Received:2015-06-06 Accepted:2015-09-28 Online:2016-05-01 Published:2016-05-24
  • Contact: Shi Heping
  • About author:

    ? These authors contributed equally to this paper

Abstract:

This study established an efficient system for inducing hairy roots and plant regeneration of Dianthus chinensis. Hairy roots could be induced from the basal surface of leaf explants of D. chinensis at 10 days after inoculation with the strain of Agrobacterium rhizogenes ATCC15834 harbouring the wild agropine-type plasmid. The percentage of rooting leaf explants was 95% at 30 days after inoculation. Hairy roots could grow rapidly and autonomously in liquid or solid plant-growth regulator-free MS medium. The transformation was confirmed by PCR amplification of the rol gene of the Ri plasmid from D. chinensis hairy roots. Hairy roots could form light green callus after culture on MS + 2.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA for 15 days. Adventitious shoots were gradually produced from calli after culture on MS + 1.0 mg·L-1 6-BA + 0.02 mg·L-1 NAA for 30 days. Compared to the control, pot-grown plants regenerated from hairy roots had shorter internodes and flowering was earlier by 18 days.