Chinese Bulletin of Botany ›› 2015, Vol. 50 ›› Issue (5): 583-590.DOI: 10.11983/CBB15062

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Role of PI3P and Its Relationship with H2O2 or Nitric Oxide in Darkness-induced Stomatal Closure in Vicia faba

Min Ma, Aijing Liu, Jie Hu, Junmin He*   

  1. School of Life Sciences, Shaanxi Normal University, Xi’an 710062, China
  • Received:2015-04-03 Revised:2015-05-11 Online:2015-09-01 Published:2015-10-09
  • Contact: Jun-Min He

Abstract: In combination with physiological and cell biological research approaches including stomatal bioassay and laser-scanning confocal microscopy, we studied the interrelationship of phosphatidylinositol 3-phosphate (PI3P) and H2O2 or nitric oxide (NO) in darkness-induced stomatal closure in epidermal strips of Vicia faba (broad bean) by using the drug treatment with wortmannin (WM) and LY294002 (LY) to inhibit the production of PI3P. The obtained results showed that both WM and LY significantly inhibited darkness-induced synthesis of H2O2 and NO in guard cells and subsequent stomatal closure but not exogenous H2O2- and NO-induced stomatal closure. In addition, exogenous treatment with H2O2 and NO completely reversed the inhibitory effect of WM and LY on darkness-induced stomatal closure. In conclusion, our results suggest that PI3P may function upstream of H2O2 and NO in the signal transduction pathways of darkness-induced stomatal closure in V. faba.