Chinese Bulletin of Botany ›› 2015, Vol. 50 ›› Issue (5): 573-582.DOI: 10.11983/CBB15091

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The Exine-dehisced Microspore Embryos Have the Proper- suspensor Differentiation in Molecular Level

Haichao Wei1,2, Yuan Liu1, Xingchun Tang3, Suxin Yang1, Mingjun Zou3, Shike Hu4, Xianzhong Feng1*   

  1. 1Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China
    2University of Chinese Academy of Sciences, Beijing 100049, China 3College of Life Sciences, Hubei University, Wuhan 430062, China
    4Hubei University of Science and Technology, Xianning 437100, China
  • Received:2015-05-28 Accepted:2015-08-21 Online:2015-09-01 Published:2015-10-09
  • Contact: Feng Xianzhong
  • About author:

    ? These authors contributed equally to this paper

Abstract: Because of sampling difficulty, research of the early stages of zygotic embryos is difficult. Previous studies showed that the exine-dehisced microspore embryogenesis system can stimulate the differentiation pattern of the zygotic embryo; an alternative approach is to use exine-dehisced microspore embryos as a substitute for zygotic embryos for relevant research work. However, we lack adequate evidence for this approach at the molecular level. In this study, we verified that the WOX family of genes could be markers of the differentiation of the embryo proper or suspensor in Brassica napus and showed that the embryo proper/suspensor in the exine-dehisced microspore embryo with suspensor could be differentiated at the molecular level by the expression pattern of the suspensor marker gene BnWOX8. These results provide solid evidence to use the exine-dehisced microspore embryogenesis system to study the early stages of the zygotic embryo. Meanwhile, we established the efficiency of the living laser microdissection technique, combined with RNA extraction from a few cells. We provide a reliable and applicable solution for detecting gene expression patterns in specific cells.