以野蔷薇(Rosa multiflora)当年生带芽茎段为试材, 建立了其组培快繁体系。结果表明, 最佳外植体是带腋芽茎段, 外植体最佳消毒方法是先用75%乙醇浸泡30秒, 再用10%次氯酸钠溶液浸泡20分钟, 成活率可达96%; 带芽茎段萌芽最佳诱导培养基为MS+1.0 mg∙L^-1 6-BA+0.01 mg∙L^-1 NAA+0.1 mg∙L^-1 GA₃, 培养30天, 萌芽率可达98%; 无菌再生苗增殖最佳基础培养基为WPM, 增殖系数为2.87; 无菌再生苗生根最佳培养基为1/2MS+1.0 mg∙L^-1 6-BA+0.1 mg∙L^-1 NAA, 生根率可达93%; 无菌再生苗移栽成活率达98%。在此基础上, 以野蔷薇无菌再生苗为受体, 建立了野蔷薇瞬时表达体系。结果表明, 瞬时表达最佳转化条件是菌液OD₆₀₀为0.8, 负压为-0.10 MPa, 真空抽吸2次, 每次15分钟, 瞬时表达效率可达96%。研究结果为建立野蔷薇再生及遗传转化体系奠定了基础, 并为蔷薇属植物基因功能研究提供技术支持。

A rapid propagation system via tissue culture for Rosa multiflora was established using the stem segments with buds of the current-year as the experimental material. The results showed that the best explants were stem segments with axillary buds. The best disinfection method was to soak the explants in 75% ethanol for 30 seconds, and then soak them in 10% sodium hypochlorite solution for 20 minutes. The survival rate can reach 96%. The optimal bud-induction medium was MS+1.0 mg∙L^-1 6-BA+0.01 mg∙L^-1 NAA+0.1 mg∙L^-1 GA₃. The budding rate can reach 98% after 30 days of cultivation. WPM was the best basal medium for the proliferation of sterile regenerated plantlets, and the proliferation coefficient was 2.87. The best medium for rooting was 1/2MS+1.0 mg∙L^-1 6-BA+0.1 mg∙L^-1 NAA, and the rooting rate can reach 93%. The transplanting survival rate of sterile regenerated plantlets was 98%. On this basis, the transient expression system of R. multiflora was established. The results showed that the optimal transformation conditions for transient expression were OD₆₀₀ of 0.8 for the bacterium culture medium, vacuum negative pressure of -0.10 MPa and vacuum suction twice for 15 minutes each time. The transient expression efficiency can reach 96%. The results of this study laid a foundation for the establishment of regeneration and genetic transformation system of R. multiflora, and also provided technical support for studying on the gene function of Rosa plants.