单细胞转录组学在植物生长发育及胁迫响应中的应用进展

王亚萍, 包文泉, 白玉娥

doi:10.11983/CBB24048

植物学报 >

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DOI: https://doi.org/10.11983/CBB24048

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单细胞转录组学在植物生长发育及胁迫响应中的应用进展

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内蒙古农业大学林学院, 呼和浩特 010019

收稿日期: 2024-03-29

修回日期: 2024-07-22

网络出版日期: 2024-08-19

基金资助

国家自然科学基金(No.32360402)和内蒙古自治区高等学校科学研究项目(No.B20231093Z)

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Advances in the Application of Single-cell Transcriptomics in Plant Growth, Development and Stress Response

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College of Forestry, Inner Mongolia Agricultural University, Huhhot 010019

Received date: 2024-03-29

Revised date: 2024-07-22

Online published: 2024-08-19

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摘要

单细胞转录组学将时空分辨率从多细胞水平转移到单细胞水平, 该技术的快速发展能够更好地揭示新的稀有细胞类型、挖掘细胞间异质性并绘制细胞发育轨迹图。目前单细胞转录组学已广泛应用于植物生长发育、应激反应和环境适应等不同研究方向, 有助于更精确、全面地揭示植物生命过程中的分子调控机制。然而, 在不同植物中的研究及分析仍面临诸多挑战。本综述比较和评估了不同的单细胞转录组技术及流程, 总结了近年来单细胞转录组学在多种植物研究的相关进展, 并探索了新型单细胞分析工具, 为以高精度和高动态探究植物生物学的研究人员提供支持。此外, 还提出了使用单细胞转录组学技术解决植物研究和育种中的一些关键技术, 及其挑战和未来发展方向。

关键词： 植物; 单细胞转录组测序; 10× Genomics; 细胞类型

本文引用格式

王亚萍, 包文泉, 白玉娥 . 单细胞转录组学在植物生长发育及胁迫响应中的应用进展[J]. 植物学报, 0 : 0 -0 . DOI: 10.11983/CBB24048

Abstract

Single-cell transcriptomics has improved the spatiotemporal resolution from multi-cell to single-cell levels, and notable progress in this technique has facilitated the identification of new rare cell types, exploration of intercellular heterogeneity, and mapping of cell developmental trajectories. Single-cell transcriptomics is currently being widely used in various research fields such as plant growth and development, stress response, and environmental adaptability, which helps to more thoroughly and precisely uncover the molecular regulatory mechanisms underlying plant life processes. However, there are numerous challenges associated with the study and analysis of different plant species. In this review, we compare and evaluate various single-cell transcription techniques and processes, summarize recent years of plant single-cell studies, and explore new single-cell analysis tools to support researchers who study plant biology with high precision and dynamics. In addition, we propose future directions for using single-cell transcriptomics technologies to address some of the key challenges in plant research and breeding. Furthermore, some important methods for addressing plant research and breeding with single-cell transcriptomics are discussed, along with their difficulties and potential applications.

Key words： Plant; single-cell RNA sequencing (scRNA-seq); 10× Genomics; cell type

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