毛建草愈伤组织诱导及植株再生
收稿日期: 2023-12-29
录用日期: 2024-05-13
网络出版日期: 2024-05-15
基金资助
山西农业大学科技创新基金(2020BQ37)
Callus Induction and Plant Regeneration of Dracocephalum rupestre
Received date: 2023-12-29
Accepted date: 2024-05-13
Online published: 2024-05-15
毛建草(Dracocephalum rupestre)是一种重要的药用植物。然而, 其叶片外植体再生系统尚未建立。以毛建草大田叶片和组培苗叶片为外植体, 探讨植物生长调节剂对愈伤组织诱导和分化、不定芽增殖及生根的影响, 建立了叶片离体再生体系。结果表明, 大田叶片愈伤组织诱导的最适培养基为MS+1.0 mg·L−1 6-BA+0.1 mg·L−1 2,4-D+1.0 mg·L−1 IAA, 诱导率达84.51%, 不定芽分化最佳培养基为MS+3.0 mg·L−1 6-BA+0.5 mg·L−1 TDZ+0.5 mg·L−1 IAA, 分化率为66.37%; 组培苗叶片愈伤组织诱导的最适培养基为MS+2.0 mg·L−1 6-BA+0.1 mg·L−1 2,4-D+0.5 mg·L−1 IAA, 诱导率达86.73%, 不定芽分化最佳培养基为MS+2.0 mg·L−1 6-BA+2.0 mg·L−1 TDZ+0.05 mg·L−1 IAA, 分化率为53.48%。不定芽增殖适宜培养基为MS+2.0 mg·L−1 6-BA+0.05 mg·L−1 NAA, 增殖率为83.57%, 最适生根培养基为1/2MS+0.1 mg·L−1 NAA+0.1 mg·L−1 IBA, 生根率为86.97%; 在草炭:蛭石=1:1 (v/v)的混合基质中组培苗长势最好。该研究建立了毛建草叶片离体培养再生体系, 为毛建草种质资源保存和种苗快繁提供了技术支持。
田旭平 , 岳康杰 , 王佳丽 , 刘慧欣 , 史子尹 , 亢红伟 . 毛建草愈伤组织诱导及植株再生[J]. 植物学报, 2024 , 59(4) : 613 -625 . DOI: 10.11983/CBB23178
Dracocephalum rupestre is an important medicinal plant. However, its leaf explant regeneration system has yet to be established. Here we investigated the impact of plant growth regulators on callus induction, differentiation, adventitious bud proliferation, and rooting using field planted leaves and tissue-cultured seedling leaves of D. rupestre as explants. We found that the optimal medium for inducing callus from field planted leaves was MS+1.0 mg·L−1 6-BA+ 0.1 mg·L−1 2,4-D+1.0 mg·L−1 IAA, achieving an induction rate of 84.51%. For adventitious bud differentiation, the preferred medium comprised MS+3.0 mg·L−1 6-BA+0.5 mg·L−1 TDZ+0.5 mg·L−1 IAA, resulting in a differentiation rate of 66.37%. Similarly, for inducing callus from tissue-cultured seedling leaves, the optimal medium included MS+2.0 mg·L−1 6-BA+0.1 mg·L−1 2,4-D+0.5 mg·L−1 IAA, with an induction rate of 86.73%. The medium conductive to adventitious bud differentiation consisted of MS+2.0 mg·L−1 6-BA+2.0 mg·L−1 TDZ+0.05 mg·L−1 IAA, yielding a differentiation rate of 53.48%. Furthermore, the appropriate medium for adventitious bud proliferation was MS+2.0 mg·L−1 6-BA+0.05 mg·L−1 NAA, achieving a proliferation rate of 83.57%. The rooting medium was 1/2MS+0.1 mg·L−1 NAA+0.1 mg·L−1 IBA, resulting in a rooting rate of 86.97%. Tissue-cultured seedlings exhibited optimal growth in a mixed substrate of peat and vermiculite at a ratio of 1:1 (v/v). This study successfully established a leaf explant regeneration system for D. rupestre, providing valuable technical support for the conservation and rapid propagation of its germplasm resources.
Key words: Dracocephalum rupestre; callus induction; differentiation; proliferation; rooting
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