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植物学报 >

2024 , Vol. 59 >Issue 3: 373 - 382

DOI: https://doi.org/10.11983/CBB23140

研究论文

西藏野生拟南芥开花时间变异的遗传基础

  • 杨继轩 ,
  • 王雪霏 ,
  • 顾红雅
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  • 北京大学生命科学学院, 蛋白质与植物基因研究国家重点实验室, 北京 100871
*顾红雅, 北京大学生命科学学院教授, 博士生导师。长期从事植物遗传多样性和演化研究, 基因家族功能和演化研究。以通讯作者身份在Cell Research、Plant Cell、New Phytologist和PLoS Genetics等植物遗传及演化相关的国际期刊上发表研究论文80余篇; 参与编著教材3部, 科普专著2部, 翻译教材3部。目前其研究团队以拟南芥为模式生物, 收集了全国约10个省份几十份野生拟南芥居群, 利用遗传学、基因组学、群体遗传学和生物信息学等手段对其遗传多样性、演化历史及适应性机制进行研究。E-mail: guhy@pku.edu.cn

收稿日期: 2023-10-15

  录用日期: 2024-01-02

  网络出版日期: 2024-01-12

基金资助

国家自然科学基金(31970242)

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Genetic Basis of Flowering Time Variations in Tibetan Arabidopsis thaliana

  • Jixuan Yang ,
  • Xuefei Wang ,
  • Hongya Gu
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  • State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China

Received date: 2023-10-15

  Accepted date: 2024-01-02

  Online published: 2024-01-12

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摘要

开花时间是被子植物生活史中的关键节点。十字花科植物拟南芥(Arabidopsis thaliana)广布于世界各地, 在海拔4 000 m以上的青藏高原也发现了该物种的自然居群, 高原独特的环境塑造了其生活史的独特表型, 在开花时间上表现为中等程度早花。该研究构建了西藏拟南芥Lhasa居群的F2代作图群体, 基于全基因组测序的QTL-seq定位分析, 在该居群中定位到主效基因FLC, 并且鉴定到其第1个内含子中存在2 307 bp的缺失, 这种单倍型只存在于西藏拟南芥居群。利用CRISPR-Cas9技术构建了Lhasa背景的flc-/-突变体, 表现为开花时间显著提前。研究结果表明, 西藏拟南芥开花时间改变的主要原因是FLC第1个内含子缺失, 该变异并未使其丧失全部功能, 这可能有利于西藏拟南芥适应青藏高原独特的气候环境。

关键词： 西藏拟南芥; 开花时间; Flowering Locus C; 适应性演化

本文引用格式

杨继轩 , 王雪霏 , 顾红雅 . 西藏野生拟南芥开花时间变异的遗传基础[J]. 植物学报, 2024 , 59(3) : 373 -382 . DOI: 10.11983/CBB23140

Abstract

Flowering time is a critical point in the life cycle of angiosperm plants. Arabidopsis thaliana of the Brassicaceae is widely distributed around the world, and the natural populations of this species have been found at altitude 4 000 m in the Qinghai-Tibet Plateau. The cold/short summer plateau climate has shaped their flowering time to be moderately early compared with those living in low altitude areas. In this study, we constructed an F2 mapping population and utilized whole-genome sequencing-based QTL-seq analysis to locate the major effect QTLs in Lhasa population of A. thaliana, and identified a haplotype-specific deletion of 2 307 bp within the first intron of FLC, which is unique to Tibetan A. thaliana. Lhasa population flc-/- mutant was constructed by CRISPR-Cas9 gene editing technique. The mutant exhibited significantly earlier flowering time than Lhasa. The above findings suggested that the deletion in the first intron of FLC in Tibetan A. thaliana was most likely the major cause for the early flowering phenotype, although it did not cause complete function loss of the FLC. This variation may have facilitated the adaptation of Tibetan A. thaliana to the unique climatic environment of the Qinghai-Tibet Plateau.

Key words： Tibetan Arabidopsis thaliana; flowering time; Flowering Locus C; adaptive evolution

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