†共同第一作者
收稿日期: 2023-05-31
录用日期: 2023-12-19
网络出版日期: 2024-01-12
基金资助
国家重点基础研究发展计划(2021YFD1200501);国家自然科学基金(32072412);科技创新2030-重大项目(2022ZD-0400203-2)
Mining and Preliminary Mapping of Rice Resistance Genes Against Bacterial Leaf Streak
†These authors contributed equally to this paper
Received date: 2023-05-31
Accepted date: 2023-12-19
Online published: 2024-01-12
由稻黄单胞菌稻生致病变种(Xanthomonas oryzae pv. oryzicola, Xoc)引起的细菌性条斑病(BLS)是水稻(Oryza sativa)生产上的重要病害, 近年来其发病呈快速上升趋势, 特别是在我国南方(包括江苏、浙江、福建和广东等地)稻区危害严重。种植抗病品种是防治BLS最理想的措施, 但目前生产上严重缺乏可用于育种的优异抗病基因资源。通过人工接种鉴定筛选水稻种质资源, 挖掘到2份高抗BLS材料(M1和D1)。多菌株系接种结果表明, M1具有非小种特异性广谱抗病(RNS BSR)特征。经遗传群体分析表明, 栽培稻M1携带单个显性抗BLS新基因Xo-3。通过混池测序和关联分析, 将Xo-3基因初步定位在2号染色体上的一段候选区域内。抗BLS种质资源的挖掘及其抗性遗传基础的解析, 将有助于理解水稻-Xoc互作机理, 从而培育抗BLS水稻新品种和制定科学的防治BLS策略。
方妍力 , 田传玉 , 苏如意 , 刘亚培 , 王春连 , 陈析丰 , 郭威 , 纪志远 . 水稻抗细菌性条斑病基因挖掘与初定位[J]. 植物学报, 2024 , 59(1) : 1 -9 . DOI: 10.11983/CBB23071
Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzicola (Xoc), is one of the most important diseases of rice, which is now highly prevalent in rice-growing regions of China, especially in Southern China (including Jiangsu, Zhejiang, Fujian, and Guangdong). Planting resistant varieties is considered as the best approach to control BLS. However, no resistant cultivars are available owing to limited genetic resources for BLS resistance. Two highly BLS-resistant materials (M1 and D1) have been discovered in our research by screening germplasm against BLS via syringe inoculation. Multi-strain inoculation showed that M1 had the characteristics of race-nonspecific broad-spectrum resistance (RNS BSR). The genetic population analysis showed that the cultivated rice M1 harbored a single dominant new gene Xo-3 of resistance to BLS. Through BSA-seq and association analysis, Xo-3 was initially mapped in a candidate region on chromosome 2. The mining of germplasm resources of BLS-resistant and the analysis of the genetic basis of its resistance will help our understanding of the interaction mechanism between rice and Xoc, so as to cultivate new varieties of BLS-resistant rice and development scientific strategies in controlling BLS.
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