苦杨×小叶杨杂交F<sub>1</sub>代苗期抗旱性比较研究

张蕾, 姜鹏飞, 王一鸣, 兰婷, 刘妍婧, 曾庆银

doi:10.11983/CBB22086

植物学报 >

2023 , Vol. 58 >Issue 4: 519 - 534

DOI: https://doi.org/10.11983/CBB22086

研究论文

苦杨×小叶杨杂交F₁代苗期抗旱性比较研究

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1.中国林业科学研究院, 林木遗传育种国家重点实验室, 北京 100091
2.北京林业大学生物科学与技术学院, 北京 100083
3.西南大学生命科学学院, 重庆 400715

收稿日期: 2022-04-23

录用日期: 2022-06-16

网络出版日期: 2022-07-17

基金资助

国家重点研发计划(2022YFD2200102);国家自然科学基金(32101552)

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Comparative Study on the Drought Resistance of Young Seedling from Populus laurifolia × P. simonii F₁ Progeny

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1. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
2. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
3. School of Life Sciences, Southwest University, Chongqing 400715, China

Received date: 2022-04-23

Accepted date: 2022-06-16

Online published: 2022-07-17

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摘要

创制抗旱林木新品种对维持我国干旱半干旱地区森林生产力, 应对全球气候变暖具有重要意义。苦杨(Populus laurifolia)是分布在新疆额尔齐斯河流域的我国乡土树种, 具有速生和耐寒等优良特性, 而小叶杨(P. simonii)具有抗旱和耐瘠薄特性。我们对苦杨×小叶杨杂交F₁代幼苗的抗旱性进行全面分析和综合评价。测定了正常生长与干旱胁迫下亲本和杂交F₁代的株高生长量和叶片相对含水量等7个生长指标, 净光合速率和胞间CO₂浓度等6个光合参数, 以及SOD活性和MDA含量等5个抗旱生化指标。对18个性状指标进行抗旱系数和隶属函数分析, 将亲本及23个F₁代幼苗划分为高、中和低3个抗旱类型。高度抗旱型幼苗的叶片、上表皮、下表皮和栅栏组织厚度较大, 叶片组织结构紧密度高, 且在干旱胁迫下抗旱关键基因的表达量显著高于其它抗旱类型幼苗。该研究为杨树抗旱育种提供了理论指导和基础材料。

关键词： 杨树; 杂交; 苗期; 抗旱性; 评价

本文引用格式

张蕾, 姜鹏飞, 王一鸣, 兰婷, 刘妍婧, 曾庆银 . 苦杨×小叶杨杂交F₁代苗期抗旱性比较研究[J]. 植物学报, 2023 , 58(4) : 519 -534 . DOI: 10.11983/CBB22086

Abstract

The development of drought-resistant tree varieties is of great significance for maintaining forest productivity in arid and semi-arid regions of China and addressing the challenges of global climate change. Populus laurifolia is distributed in the Irtysh River Basin in Xinjiang, China, has excellent characteristics such as fast growth and cold resistance. P. simonii has the characteristics of drought resistance and barren resistance, and is widely planted in northern China. In this study, the drought resistance characteristics of P. laurifolia × P. simonii F₁ progeny were comprehensively analyzed and evaluated. We investigated seven growth parameters such as high growth and relative leaf water content, six photosynthetic parameters such as net photosynthetic rate and intercellular CO₂ concentration, and five biochemical characteristics such as SOD activity and MDA content. Based on the drought resistance coefficient and membership function analysis of 18 trait parameters, the parent and 23 F₁ progeny were divided into three drought resistance types: high, medium and low. Compared to other two drought resistance types seedlings, the highly drought-resistant F₁ progeny seedlings had larger leaf thickness, upper and lower epidermis thickness, palisade tissue thickness, and leaf tissue compactness. For highly drought-resistant F₁ progeny seedlings, the expression levels of key drought resistance genes under drought stress were significantly higher than other two drought resistance types. This study provides theoretical guidance and material basis for breeding drought-resistant poplar.

Key words： poplar; hybrid; seedling stage; drought resistance; evaluation

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