以粉美人萱草(Hemerocallis fulva cv. ‘Fenmeiren’)的花茎为外植体进行离体培养, 该研究成功建立了粉美人萱草组培快繁技术。结果表明, 6月获得的外植体用浓度为15% (v/v)的次氯酸钠溶液消毒8分钟, 外植体存活率达95%; 最佳增殖培养基为MS+1.0 mg·L^-1 6-BA+0.004 mg·L^-1 TDZ+0.1 mg·L^-1 NAA, 培养30天后, 月增殖系数达2.9; 壮苗培养基为MS+0.1 mg·L^-1 6-BA+0.1 mg·L^-1 IBA, 在该培养基中, 组培苗不再分化, 长势健壮; 最佳生根培养基为1/2MS+0.4 mg·L^-1 IBA+20 g·L^-1蔗糖, 生根率达95%; 移栽基质采用珍珠岩:草炭=1:2 (v/v), 通过精细化管理, 成活率可达85%, 出圃合格率为75%。目前已实现规模化繁殖, 并生产组培苗2.0×10⁵株, 大田种植表现良好。

Using the scape as explants, we successfully established the tissue culture and rapid propagation technology for the large-scale production of Hemerocallis fulva cv. ‘Fenmeiren’. Our results showed that the survival rate of explant was 95%, which explants were obtained in June and sterilized in the 15% (v/v) sodium hypochlorite solution for 8 minutes. The best proliferation medium was MS + 1.0 mg·L^-1 6-BA + 0.004 mg·L^-1 TDZ + 0.1 mg·L^-1 NAA. After 30 days of cultivation, the monthly proliferation coefficient reached 2.9. On the medium of MS + 0.1 mg·L^-1 6-BA + 0.1 mg·L^-1 IBA, the tissue culture seedlings did not differentiate and grew vigorously. The optimal rooting medium was 1/2MS + 0.4 mg·L^-1 IBA + 20 g·L^-1 sucrose, and the rooting rate was 95%. by use of perlite:peat=1:2 (v/v) as the transplanting matrix, the survival rate of transplanting was 85% and the qualified rate was 75%. At present, the large-scale production of this cultivar has been achieved and 2.0 × 10⁵ tissue culture seedlings were produced, which all performed well in the field.