粉美人萱草的快繁技术和大田种植

doi:10.11983/CBB21182

植物学报 ›› 2022, Vol. 57 ›› Issue (3): 350-357.DOI: 10.11983/CBB21182 cstr: 32102.14.CBB21182

粉美人萱草的快繁技术和大田种植

吕秀立¹, 于泽群², 陈香波¹, 傅仁杰¹, 缪珊珊³^,^*(), 杜安³^,^*()

¹上海市园林科学规划研究院, 上海 200232
²上海园林绿化建设有限公司, 上海 200333
³上海市园林设计研究总院有限公司, 上海 200031

收稿日期:2021-10-22 接受日期:2022-04-20 出版日期:2022-05-01 发布日期:2022-05-18
通讯作者: 缪珊珊,杜安
作者简介:496466875@qq.com
* E-mail: 545042581@qq.com;
第一联系人：^† 共同第一作者
基金资助:
国家林业和草原局林草国家创新联盟自筹研发项目(GLM [2021] 101)

Rapid Propagation Technology and Field Production of Hemerocallis fulva cv. ‘Fenmeiren’

Lü Xiuli¹, Yu Zequn², Chen Xiangbo¹, Fu Renjie¹, Miao Shanshan³^,^*(), Du An³^,^*()

¹Shanghai Academy of Landscape Architecture Science and Planning, Shanghai 200232, China
²Shanghai Gardening-Landscaping Construction Co, Ltd, Shanghai 200333, China
³Shanghai Landscape Architecture Design Research Institute Co, Ltd, Shanghai 200031, China

Received:2021-10-22 Accepted:2022-04-20 Online:2022-05-01 Published:2022-05-18
Contact: Miao Shanshan,Du An
About author:First author contact:^† These authors contributed equally to this paper.

摘要/Abstract

摘要： 以粉美人萱草(Hemerocallis fulva cv. ‘Fenmeiren’)的花茎为外植体进行离体培养, 该研究成功建立了粉美人萱草组培快繁技术。结果表明, 6月获得的外植体用浓度为15% (v/v)的次氯酸钠溶液消毒8分钟, 外植体存活率达95%; 最佳增殖培养基为MS+1.0 mg·L^-1 6-BA+0.004 mg·L^-1TDZ+0.1 mg·L^-1 NAA, 培养30天后, 月增殖系数达2.9; 壮苗培养基为MS+0.1 mg·L^-16-BA+0.1 mg·L^-1 IBA, 在该培养基中, 组培苗不再分化, 长势健壮; 最佳生根培养基为1/2MS+0.4 mg·L^-1 IBA+20 g·L^-1蔗糖, 生根率达95%; 移栽基质采用珍珠岩:草炭=1:2 (v/v), 通过精细化管理, 成活率可达85%, 出圃合格率为75%。目前已实现规模化繁殖, 并生产组培苗2.0×10⁵株, 大田种植表现良好。

关键词: 粉美人萱草, 快繁技术, 规模化繁殖, 大田种植

Abstract: Using the scape as explants, we successfully established the tissue culture and rapid propagation technology for the large-scale production of Hemerocallis fulva cv. ‘Fenmeiren’. Our results showed that the survival rate of explant was 95%, which explants were obtained in June and sterilized in the 15% (v/v) sodium hypochlorite solution for 8 minutes. The best proliferation medium was MS + 1.0 mg·L^-1 6-BA + 0.004 mg·L^-1 TDZ + 0.1 mg·L^-1 NAA. After 30 days of cultivation, the monthly proliferation coefficient reached 2.9. On the medium of MS + 0.1 mg·L^-16-BA + 0.1 mg·L^-1 IBA, the tissue culture seedlings did not differentiate and grew vigorously. The optimal rooting medium was 1/2MS + 0.4 mg·L^-1 IBA + 20 g·L^-1 sucrose, and the rooting rate was 95%. by use of perlite:peat=1:2 (v/v) as the transplanting matrix, the survival rate of transplanting was 85% and the qualified rate was 75%. At present, the large-scale production of this cultivar has been achieved and 2.0 × 10⁵ tissue culture seedlings were produced, which all performed well in the field.

Key words: Hemerocallis fulva cv. ‘Fenmeiren’, rapid propagation technology, large-scale production, field planting

吕秀立, 于泽群, 陈香波, 傅仁杰, 缪珊珊, 杜安. 粉美人萱草的快繁技术和大田种植. 植物学报, 2022, 57(3): 350-357.

Lü Xiuli, Yu Zequn, Chen Xiangbo, Fu Renjie, Miao Shanshan, Du An. Rapid Propagation Technology and Field Production of Hemerocallis fulva cv. ‘Fenmeiren’. Chinese Bulletin of Botany, 2022, 57(3): 350-357.

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参考文献 19

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Sterilization time (min)	Number of inoculation	Survival number	Survival rate (%)
4	20	2±1 d	10±0.05 d
6	20	5±1 c	25±0.05 c
8	20	9±1 a	45±0.05 a
10	20	7±0 b	35±0 b
12	20	5±0 c	25±0 c

Sterilization time (min)	Number of inoculation	Survival number	Survival rate (%)
4	20	2±1 d	10±0.05 d
6	20	5±1 c	25±0.05 c
8	20	9±1 a	45±0.05 a
10	20	7±0 b	35±0 b
12	20	5±0 c	25±0 c

Disinfectant concentration (%)	Number of inoculation	Survival number	Survival rate (%)
8	20	8±1 d	40±0.05 d
10	20	9±1 c	45±0.05 c
15	20	12±1 a	60±0.05 a
18	20	10±0.05 b	50±0.05 b
20	20	7±2 e	35±0.1 e

Disinfectant concentration (%)	Number of inoculation	Survival number	Survival rate (%)
8	20	8±1 d	40±0.05 d
10	20	9±1 c	45±0.05 c
15	20	12±1 a	60±0.05 a
18	20	10±0.05 b	50±0.05 b
20	20	7±2 e	35±0.1 e

Sampling date	Number of inoculation	Survival number	Survival rate (%)
April 1	20	8±1 e	40±0.05 e
April 15	20	12±1 c	60±0.05 c
May 1	20	16±3 b	80±0.15 b
May 15	20	19±1 a	95±0.05 a
June 1	20	9±1 d	45±0.05 d
June 15	20	3±1 f	15±0.05 f

粉美人萱草的快繁技术和大田种植

Rapid Propagation Technology and Field Production of Hemerocallis fulva cv. ‘Fenmeiren’

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Medium	6-BA (mg·L^-1)	TDZ (mg·L^-1)	NAA (mg·L^-1)	Plantlet No.	No. of multiplication seedlings	Propagation rate
MS	0.1	0	0.1	20	3±1 g	0.15±0.05 g
MS	0.5	0	0.1	20	9±1 f	0.45±0.05 f
MS	1.0	0	0.1	20	18±1 de	0.9±0.05 de
MS	2.0	0	0.1	20	13±1 e	0.65±0.05 e
MS	1.0	0.001	0.1	20	23±1 cd	1.15±0.05 cd
MS	1.0	0.002	0.1	20	45±1 c	2.25±0.05 c
MS	1.0	0.004	0.1	20	58±1 b	2.9±0.05 b
MS	1.0	0.008	0.1	20	98±1 a	4.9±0.05 a

Medium	6-BA (mg·L^-1)	IBA (mg·L^-1)	NAA (mg·L^-1)	Callus	State difference
MS	0.1	0	0.1	A little	Undifferentiation, no root
MS	0.2	0	0.1	A little	Differentiation, no root
MS	0.1	0.1	0	No	Undifferentiation, no root
MS	0.2	0.1	0	No	Differentiation, no root
1/2MS	0.1	0	0.1	A little	Undifferentiation, no root
1/2MS	0.2	0	0.1	A little	Differentiation, no root
1/2MS	0.1	0.1	0	No	Undifferentiation, no root
1/2MS	0.2	0.1	0	No	Differentiation, no root

Medium	IBA (mg·L^-1)	Sucrose (g·L^-1)	Plant- let No.	No. of roo- ting buds	Rooting rate (%)
MS	0.2	30	20	7±1 e	35±0.05 e
MS	0.4	30	20	11±1 c	55±0.05 c
MS	0.8	30	20	14±1 b	70±0.05 b
MS	0.4	10	20	7±1 e	35±0.05 e
MS	0.4	20	20	15±1 b	75±0.05 b
MS	0.4	40	20	9±1 d	45±0.05 d
1/2MS	0.4	20	20	19±1 a	95±0.05 a

Transplanting cycle	Relative hu- midity (%)	Shade degrees (%)	Ventilation time (h)
The first week	95	80	0.5
The second week	85	70	1
The third week	75	60	2
The fourth week	65	50	4
The fifth to sixth weeks	55	40	6
The seventh to eighth weeks	35	20	12

Batch	Multiple shoots	Regenerated plantlets	Proliferation coefficient	Rooting rate (%)	Transplanting survival rate (%)	Qualified rate (%)
1	16190	48333	2.8	93	83	73
2	18279	53010	2.9	92	82	72
3	18337	55012	3.0	96	87	76
4	18749	58123	3.1	97	88	75
5	17390	68693	2.8	93	84	77
Average	17789	56634	2.92	94.2	84.8	74.6