气温和根区温度对葡萄叶片光合荧光特性的影响

王浩, 王明, 梁婷, 姚玉新, 杜远鹏, 高振

doi:10.11983/CBB21122

植物学报 >

2022 , Vol. 57 >Issue 2: 209 - 216

DOI: https://doi.org/10.11983/CBB21122

研究报告

气温和根区温度对葡萄叶片光合荧光特性的影响

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¹山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室, 山东果蔬优质高效生产协同创新中心, 泰安 271018
²山东省泰安市岱岳区农业农村局, 泰安 271021

gaoz89@sdau.edu.cn
*E-mail: duyuanpeng001@163.com;

收稿日期: 2021-07-25

录用日期: 2021-12-28

网络出版日期: 2021-12-28

基金资助

国家重点研发计划(2019YFD1000101);财政部和农业农村部和国家现代农业产业技术体系(CARS-29-zp-1)

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Effects of High Air and Root Zone Temperature on Photosynthetic Fluorescence Characteristics of Grape Leaves

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¹Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production in Shandong, State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
²Daiyue District Bureau of Agriculture and Rural Areas, Tai’an City, Shandong Province, Tai’an 271021, China

Received date: 2021-07-25

Accepted date: 2021-12-28

Online published: 2021-12-28

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摘要

为探究气温和根区温度对葡萄(Vitis vinifera)叶片光合荧光特性的影响, 以一年生巨峰葡萄为试材, 设置对照、高气温、高根区温度和两者交叉作用共4组处理。结果表明, 相较于对照和高气温, 高根区温度以及交叉处理叶片最大光化学效率(F_v/F_m)降低更明显; 与对照相比, 高根区温度以及高气温与高根区温度交叉处理下光系统II (PSII)实际光化学效率Y(II)显著降低, 非调节能量耗散的量子产量Y(NPQ)及Q_A氧化还原状态(1-q_P)值显著上升。同时, 高根区温度以及高气温与高根区温度交叉处理显著增加了J点的可变荧光(V_j), 而用于电子传递的量子产额(φ_Eo)及性能指数(PI_ABS)显著降低。此外, 高根区温度以及高气温与高根区温度交叉处理下单位面积有活性的反应中心数目(RC/CS_m)也显著下降, K点相对可变荧光(W_k)明显上升。综上所述, 高根区温度是高气温与根区高温交叉胁迫的主导因子, PSII受体侧是主要的伤害位点, 高气温加剧了高根区温度对PSII造成的伤害。

关键词： 葡萄; 空气高温; 根区高温; 叶绿素荧光

本文引用格式

王浩, 王明, 梁婷, 姚玉新, 杜远鹏, 高振 . 气温和根区温度对葡萄叶片光合荧光特性的影响[J]. 植物学报, 2022 , 57(2) : 209 -216 . DOI: 10.11983/CBB21122

Abstract

In order to explore the effects of different air and root zone temperature stress on the fluorescence characteristics of grape (Vitis vinifera) leaves, we treated potted annual Kyoho seedlings under four different temperature conditions: control (C), high air temperature (HA), high root zone temperature (HR), and combined treatment (CT) of HA and HR. Our results showed that the maximum photochemical efficiency (F_v/F_m) under HR and CT treatments was more significantly reduced than under C and HA conditions. The actual quantum efficiency Y(II) of the PSII under HR and CT were significantly reduced, while the quantum yield Y(NPQ) of the non-adjusted energy dissipation, and the redox state of Q_A (1-q_p) were significantly increased compared with the control. At the same time, the variable fluorescence (V_j) of the J point was increased significantly under HR and CT treatments, while the quantum yield (φ_Eo) used for electron transfer and the performance index based on absorption of light energy (PI_ABS) were significantly reduced. In addition, the number of active reaction centers per unit area (RC/CS_m) under HR and CT treatments was decreased significantly, while the relative variable fluorescence (W_k) after normalization was increased significantly. Taken together, our study indicated that high temperature in root zone is a key stress affecting leaf fluorescence characteristics by causing damage on the PSII receptor side. High air temperature aggravated the damage on PSII caused by high root zone temperature.

Key words： grapes; high air temperature; high root zone temperature; chlorophyll fluorescence

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