RNA编辑是一种转录后修饰加工过程, 通过碱基的插入、缺失或替换可改变氨基酸的种类, 增加蛋白质的疏水性和同源蛋白在不同物种间的保守性。该文通过DNA与cDNA序列的比对, 分析了裸子植物银杏(Ginkgo biloba)叶绿体功能基因ndhF的编辑现象, 该基因共含有21个编辑位点, 且这21个位点均为部分编辑。生物信息学分析及与其它物种比对结果表明, ndhF C290位编辑可能会影响该蛋白的正确折叠。进一步使用单克隆酶切方法测定了不同胁迫处理对ndhF C290位编辑效率的影响, 结果表明该位点的编辑效率对温度和黑暗敏感。
RNA editing is a post-transcriptional modification process including nucleotide insertion, deletion or replacement. Mostly, RNA editing converts amino acid from hydrophilic to hydrophobic and restores conservation of homologous amino acid residues among different species. In this paper, we analyzed RNA editing sites of ndhF gene of chloroplast in Ginkgo biloba by PCR and RT-PCR. The results show that total 21 editing sites are found in this gene. All 21 sites are partially edited with C to U conversions. Among of 21 editing sites, C290 is probably a critical editing site for ndhF to form adapted secondary and tertiary structure by using bioinformatics analysis. We further examined ndhF C290 editing efficiency with enzyme digestion method under low or high temperature, salt, drought and darkness treatment. The results show that ndhF C290 editing efficiency is significantly affected by temperature and darkness than by salt and drought treatment.
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