Abstract: RNA editing is a post-transcriptional modification process including nucleotide insertion, deletion or replacement. Mostly, RNA editing converts amino acid from hydrophilic to hydrophobic and restores conservation of homologous amino acid residues among different species. In this paper, we analyzed RNA editing sites of ndhF gene of chloroplast in Ginkgo biloba by PCR and RT-PCR. The results show that total 21 editing sites are found in this gene. All 21 sites are partially edited with C to U conversions. Among of 21 editing sites, C290 is probably a critical editing site for ndhF to form adapted secondary and tertiary structure by using bioinformatics analysis. We further examined ndhF C290 editing efficiency with enzyme digestion method under low or high temperature, salt, drought and darkness treatment. The results show that ndhF C290 editing efficiency is significantly affected by temperature and darkness than by salt and drought treatment.