收稿日期: 2019-10-24
录用日期: 2019-12-31
网络出版日期: 2020-01-03
基金资助
国家自然科学基金(No.31670248);国家自然科学基金(No.31970276)
Protocols for Analyzing Plant Phospho-proteins
Received date: 2019-10-24
Accepted date: 2019-12-31
Online published: 2020-01-03
朱丹,曹汉威,李媛,任东涛 . 植物蛋白磷酸化的检测方法[J]. 植物学报, 2020 , 55(1) : 76 -82 . DOI: 10.11983/CBB19208
Protein phosphorylation is one of the important protein posttranslational modifications that is involved in the regulation of most cellular processes in plants. Protein kinases catalyze the phosphorylation by transferring the phosphate group in ATP to the substrate proteins. The phosphate is usually covalently linked to the hydroxyl group of specific amino acid residues in the substrates by an ester bond. The mostly studied phosphorylation sites are serine, threonine, and tyrosine residues. Here, we present protocols and related tips for the in vitro and in vivo protein phosphorylation assays.
Key words： protein phosphorylation; protein kinase; plant
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