收稿日期: 2019-11-24
录用日期: 2019-12-02
网络出版日期: 2019-12-04
基金资助
国家自然科学基金(31471741)
Luciferase Complementation Assay for Detecting Protein Interactions
Received date: 2019-11-24
Accepted date: 2019-12-02
Online published: 2019-12-04
赵燕,周俭民 . 萤火素酶互补实验检测蛋白互作[J]. 植物学报, 2020 , 55(1) : 69 -75 . DOI: 10.11983/CBB19229
Protein-Protein interactions play important roles in various eukaryotic biological processes. Compared to other techniques measuring protein-protein interactions in plants, the Luciferase Complementation Assay (LCA), based on Agrobacterium-mediated transient expression in Nicotiana benthamiana, is a simple, sensitive, reliable, highly quantitative and low background method that can be easily scaled up for high-throughput interactome studies. Here, we describe a protocol that includes two alternative data collection methods to qualitative and quantitative analyse luminescence or luminous intensity to detect protein-protein interactions in plant cells.
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