基于转录组分析鉴定苹果茉莉素响应基因
- 1 山东农业大学园艺科学与工程学院/作物生物学国家重点实验室, 泰安 271018
2 泰山学院生物与酿酒工程学院, 泰安 271018
收稿日期: 2018-11-04
录用日期: 2019-02-11
网络出版日期: 2019-02-21
基金资助
国家重点研发计划(No.2018YFD1000307);国家自然科学基金(No.31272132);山东省泰山学者工程启动基金(No.tshw20120712)
Identifying Genes Responsive to Jasmonates in Apple Based on Transcriptome Analysis
- 1 State Key Laboratory of Crop Biology, College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
2 College of Biology and Brewing Engineering, Taishan University, Tai’an 271018, China
Received date: 2018-11-04
Accepted date: 2019-02-11
Online published: 2019-02-21
摘要
为阐明外源茉莉酸甲酯(MeJA)诱导的苹果(Malus domestica)抗病分子机制, 以生长30天的Gala组培苗为试材, 用100 μmol∙L -1MeJA处理叶片12小时, 通过转录组测序, 结合生物信息学分析鉴定出苹果叶片中受MeJA诱导表达的基因。结果表明, 外源MeJA主要影响苹果叶片倍半萜类、三萜和类黄酮的生物合成, 以及芸薹素(BR)信号转导途径间接诱导的抗病性; 倍半萜类、三萜及类黄酮生物合成途径中的关键基因为MDP0000702120和MDP0000692178; MDP0000123379是联系芸薹素信号转导途径和植物-病原菌互作途径的关键调控基因。
本文引用格式
张娜,刘秀霞,陈学森,吴树敬 . 基于转录组分析鉴定苹果茉莉素响应基因[J]. 植物学报, 2019 , 54(6) : 733 -743 . DOI: 10.11983/CBB18235
Abstract
To study the molecular mechanism of resistance induced by exogenous methyljasmonate (MeJA) in apple, tissue cultured seedlings of Gala grown for 30 days were used as materials. The leaves were treated with 100 μmol∙L -1 MeJA for 12 h. RNA sequencing and bioinformatics analysis were conducted to identify differentially expressed genes induced by MeJA. Exogenous MeJA mainly affected the biosynthesis of sesquiterpenoids, triterpenoids and flavonoids in apple leaves and induced disease resistance by brassinolide signal transduction pathways indirectly. The key genes regulating sesquiterpenoid and triterpenoid biosynthesis and flavonoids biosynthesis are MDP0000702120 and MDP0000692178; MDP0000123379 was the key regulatory gene linked to the brassinosteroid signaling pathway and plant-pathogen interaction pathway.
Key words： jasmonates; disease resistance; signal transduction; transcriptome; apple
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