芒(Miscanthus sinensis)具较高的生物学产量, 是一种极具发展前景的纤维素类能源植物。以芒的8种不同基因型幼穗为外植体, 进行了组织培养研究。结果表明, 不同基因型芒在愈伤组织诱导率、胚性愈伤组织诱导率和胚性愈伤组织分化率等方面均存在显著差异。W89和W70均具较高的愈伤组织诱导率, 分别为91.7%和89.1%; W69的外植体几乎全部褐化, 且未能诱导出愈伤组织。W89、W70和W17的胚性愈伤组织分化率较高, 达50%以上。另外, 发现愈伤组织诱导率与细胞壁木质素含量间呈显著的负相关。该研究建立了稳定且有效的再生体系, 并初步确定W89和W70可作为芒组织培养的理想材料, 为芒的遗传转化、定向改良和良种快繁提供了技术支持。

Miscanthus sinensis has high biomass yield and is currently considered a leading energy crop candidate around the world. We performed conventional tissue culture with immature inflorescence tissue from 8 genotypes of M. sinensis and found distinct frequencies in callus induction, embryogenic-like callus induction and embryogenic-like callus differentiation. In particular, W89 and W70 showed significantly high callus induction, at 91.7% and 89.1%, respectively, whereas explants of W69 had a brownish appearance, with barely any callus. Furthermore, W89, W70 and W17 showed relatively high percentages of embryogenic-like callus differentiation (50%) with easy plantlet regeneration. Callus induction rates were affected by lignin level. The established system for efficient micropropagation could be used for genetic engineering, improvement and plant propagation of M. sinensis, and 2 genotypes, W89 and W70, may be model materials for plant tissue culture.