采用近红外漫反射光谱技术对淫羊藿(Epimedium)的蛋白质含量进行快速且无损检测。近红外漫反射光谱经二阶导数处理、标准多元离散校正及主成分分析聚类处理后, 采用改进最小二乘法回归得到的定标模型预测效果最佳, 定标决定系数、交互验证标准差及交互验证相关系数分别为0.923、0.554和0.717。近红外光谱分析法的测定结果与用凯氏定氮法所得结果无显著差异, 两种方法测定值的相关性较高(R2=0.933 9)。重复性实验表明, 近红外光谱分析法的相对标准偏差为0.937%。该研究首次采用近红外光谱分析法测定了8种淫羊藿的蛋白质含量。该方法简便、精确, 在淫羊藿资源开发利用和药材质量控制方面具有参考意义。
Near-infrared reflectance spectroscopy was used for rapid and nondestructive determination of proteins in Epimedium. The calibration model was developed under the following conditions: second-derivative, standard multiplicative scatter correction, principal component analysis and modified partial least-squares regression. R squared, standard error of cross-validation and 1 minus variance ratio values were 0.923, 0.554 and 0.717, respectively. Protein content did not differ by near-infrared reflectance spectroscopy or Kjedahl nitrogen determination methods, and their relationship was close (R2=0.933 9). The proposed near-infrared reflectance spectroscopy method is a simple analytical technique with satisfactory accuracy and repeatability (RSD=0.937%). Protein content in 8 species of Epimedium were determined by near-infrared reflectance spectroscopy. The method shows great potential for protein assay in quality control and resource utilization of Epimedium.
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