植物学报 ›› 2014, Vol. 49 ›› Issue (5): 611-617.DOI: 10.3724/SP.J.1259.2014.00611

• 技术方法 • 上一篇    下一篇

基于近红外漫反射光谱快速测定淫羊藿蛋白质含量

牛丽丽1, 张华峰1*, 陈乐1, 杨晓华2, 李璐1   

  1. 1陕西师范大学食品工程与营养科学学院, 药用资源与天然药物化学教育部重点实验室, 西北濒危药材资源开发国家工程实验室, 西安 710062;
    2西安交通大学医学院, 卫生部法医学重点实验室, 西安 710061
  • 收稿日期:2013-08-21 修回日期:2013-11-07 出版日期:2014-09-01 发布日期:2014-07-02
  • 通讯作者: 张华峰
  • 基金资助:

    教育部中央高校基本科研业务费专项资金项目;陕西师范大学科学研究项目

Rapid Quantification of Protein in Epimedium by Near-infrared Reflectance Spectroscopy

Lili Niu1, Huafeng Zhang1*, Le Chen1, Xiaohua Yang2, Lu Li1   

  1. 1National Engineering Laboratory for Resources Development of Endangered Crude Drugs in Northwest China, Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China;

    2Key Laboratory of Ministry of Health for Forensic Sciences, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China
  • Received:2013-08-21 Revised:2013-11-07 Online:2014-09-01 Published:2014-07-02
  • Contact: Huafeng Zhang

摘要: 采用近红外漫反射光谱技术对淫羊藿(Epimedium)的蛋白质含量进行快速且无损检测。近红外漫反射光谱经二阶导数处理、标准多元离散校正及主成分分析聚类处理后, 采用改进最小二乘法回归得到的定标模型预测效果最佳, 定标决定系数、交互验证标准差及交互验证相关系数分别为0.923、0.554和0.717。近红外光谱分析法的测定结果与用凯氏定氮法所得结果无显著差异, 两种方法测定值的相关性较高(R2=0.933 9)。重复性实验表明, 近红外光谱分析法的相对标准偏差为0.937%。该研究首次采用近红外光谱分析法测定了8种淫羊藿的蛋白质含量。该方法简便、精确, 在淫羊藿资源开发利用和药材质量控制方面具有参考意义。

Abstract: Near-infrared reflectance spectroscopy was used for rapid and nondestructive determination of proteins in Epimedium. The calibration model was developed under the following conditions: second-derivative, standard multiplicative scatter correction, principal component analysis and modified partial least-squares regression. R squared, standard error of cross-validation and 1 minus variance ratio values were 0.923, 0.554 and 0.717, respectively. Protein content did not differ by near-infrared reflectance spectroscopy or Kjedahl nitrogen determination methods, and their relationship was close (R2=0.933 9). The proposed near-infrared reflectance spectroscopy method is a simple analytical technique with satisfactory accuracy and repeatability (RSD=0.937%). Protein content in 8 species of Epimedium were determined by near-infrared reflectance spectroscopy. The method shows great potential for protein assay in quality control and resource utilization of Epimedium.