基于LA-ICP-MS的文旦柚果实养分原位可视化与半定量分析新方法

王寒雪; 陈昊; 洪佳伟; 陈玥勤; 廖红

doi:10.11983/CBB25113

植物学报 >

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DOI: https://doi.org/10.11983/CBB25113

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基于LA-ICP-MS的文旦柚果实养分原位可视化与半定量分析新方法

王寒雪 ,
陈昊 ,
洪佳伟 ,
陈玥勤 ,
廖红

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福建农林大学资源与环境学院, 根系生物学研究中心, 福州 350000

收稿日期: 2025-06-19

修回日期: 2025-08-12

网络出版日期: 2025-10-22

基金资助

校创基金(No.105-KFB24130A)

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Visualization and Semi-quantitative Analysis of in-situ Distribution of Nutrients in Wendan Pomelo Fruit Based on LA-ICP-MS

YU Han-Xue ,
CHEN Hao ,
HONG Jia-Wei ,
CHEN Yue-Qi ,
LIAO Hong

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Root Biology Center, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received date: 2025-06-19

Revised date: 2025-08-12

Online published: 2025-10-22

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摘要

原位可视化果实养分浓度, 有助于解析果实养分分配及需求规律, 为果树科学合理施肥提供理论依据。本研究建立了一种基于激光剥蚀-电感耦合等离子体质谱(LA-ICP-MS)、原位可视化文旦柚果实养分浓度的新方法。该方法使用石蜡切片方法进行前期处理避免由于果实柔软、多汁等导致组织破损的问题; 使用飞秒激光器, 以研究对象的空间分辨率为主, 通过不同组织离子强度展示各养分的具体分布, 并且使用外标法进行基体校正并提供柑橘属中养分元素的相对含量, 原位半定量并可视化分析了文旦柚果实不同部位不同组织的养分浓度。结果表明, 文旦柚果实中, 钾、氮、钙、镁和磷浓度较高, 主要集于黄皮层的角质层、白皮层的维管束类组织及汁胞的表皮组织。其中, 钙相对平均浓度在黄皮层高达516.21±387.40 mg∙L^–1, 锌相对平均浓度在黄皮层的角质层上可达410.22±279.77 mg∙L^–1, 二者在汁胞中表皮细胞相对浓度最高, 暗示钙和锌在文旦柚果实中具有重要的营养作用。

关键词： 原位可视化; 激光剥蚀-电感耦合等离子体质谱法; 半定量方法; 养分分布

本文引用格式

王寒雪 , 陈昊 , 洪佳伟 , 陈玥勤 , 廖红 . 基于LA-ICP-MS的文旦柚果实养分原位可视化与半定量分析新方法[J]. 植物学报, 0 : 1 -0 . DOI: 10.11983/CBB25113

Abstract

INTRODUCTION: The distribution and concentration of nutrient elements within fruits directly influence their yield and quality. Conventional analytical methods primarily rely on digestion to quantify nutrient content in different fruit tissues. However, as fruits comprise multiple tissue structures, basic quantification techniques fail to precisely resolve nutrient distribution across these microstructural compartments. Consequently, establishing an in-situ method enabling visualization of spatial nutrient concentration profiles in fruit tissues is imperative.

RATIONALE: Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS), as an advanced micro-analytical technique, enables effective visualization of in situ elemental distribution within plant tissues. LA-ICP-MS uses a femtosecond laser (wave-length 343nm) to scan the fruit sample and the standard sample according to the line of the laser beam during the measurement of the femtosecond laser (wave-length 343nm). The burned sample material is sent to the ICP with the carrier gas Ar, and the ionic strength of P³¹, K³⁹, Ca⁴⁴, Mg²⁴, Fe⁵⁷, Mn⁵⁵, Cu⁶³, Zn⁶⁶, S³⁴, Al²⁷, Cr⁵², Mo⁹⁵, Pb²⁰⁸, Sr⁸⁸ and Rb⁸⁵ in different parts of the fruit is detected. Visualize the nutrient distribution between different tissues.

RESULTS: Through LA-ICP-MS analysis, we performed in situ, spatially-resolved and semi-quantitative analysis of nutritional element distributions across distinct tissue structures in pomelo fruit during maturation. Significant tissue-specific heterogeneity was observed in elemental distributions among fruit compartments. Three structures demonstrated consistent nutrient enrichment: cuticle of flavedo, vascular bundle-like structures in albedo, epidermal cells of juice sacs. The concentrations of potassium, nitrogen, calcium, magnesium and phosphorus were higher in the fruit of Wendan pomelo, which were mainly concentrated in the cuticle of flavedo, vascular bundle tissue of albedo and epidermal tissue of juice sac. Among them, the relative average concentration of calcium in the yellow cortex was as high as 516.21 ± 387.40 mg ·L^-1, and the relative average concentration of zinc in the cuticle of the yellow cortex was 410.22 ± 279.77 mg ·L^-1. The relative concentration of the two in the epidermal cells of the juice sac was the highest, suggesting that calcium and zinc play an important nutritional role in the fruit of Wendan pomelo.

CONCLUSION: This study established a novel LA-ICP-MS methodology for in-situ, visual and semi-quantitative analysis for nutrient distribution in different tissue structures of fruits, was used to establish the spatial relationship of nutrient distribution, which filled the gap in the visualization of fruit nutrient distribution. The relative concentration of different elements in each tissue was analyzed, which provided scientific theory for the subsequent guidance of Wendan pomelo farming measures.

LA-ICP-MS in situ visualization and semi-quantitative calcium concentration in various parts of fruit. The in situ distribution of calcium element in the flavedo, albedo and juice sac and the concentration of tissue structure were analyzed.

Key words： In-situ visualization,; laser ablation-inductively coupled plasma mass spectrometry,; semi-quantitative method,; nutrient distribution

参考文献

[1]Diniz A P, Kozovits A R, Lana C D C, Abreu A T D, Leite M G P(2019).Quantitative analysis of plant leaf elements using the LA-ICP-MS technique.Int.J.Mass Specom, 435:251-258. [2]Hrdli?ka A, Hegrová J, Bucková M, Prochazka D, Holá M, Novotny K, Po?ízka P, Kanicky V, Kaiser J(2022).Feasibility of direct analysis of algae contamination with chromium and copper on the filter with laser-induced breakdown spectroscopy and laser ablation inductively coupled plasma mass spectrometry..Spectrochimica Acta Part B: Atomic Spectroscopy, 195:1-8. [3]Becker J, Zoriy M, Dressler V, Wu B, Becker J(2008).Imaging of metals and metal-containing species in biological tissues and on gels by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS): A new analytical strategy for applications in life sciences.Pure Appl. Chem, 80:2643-2655. [4]Becker JS, Zoriy M, Matusch A, Wu B, Salber D, Palm C, Becker JS(2010).Bioimaging of metals by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS).Mass Spectrom Rev, 29:156-175. [5]Becker J S, Matusch A, Wu B(2014).Bioimaging mass spectrometry of trace elements - recent advance and applications of LA-ICP-MS: A review.Anal Chim Acta, 835:1-18. [6]Cao S, Zeng B, Zhou X, Deng S, Zhang W, Luo S, Ouyang M, Yang S(2024).Multivariate Analysis and Optimization Scheme of the Relationship between Leaf Nutrients and Fruit Quality in ‘Bingtang’ Sweet Orange Orchards.Horticulturae, 10:976-989. [7]Che J, Yamaji N, Wang S F, Xia Y, Yang S Y, Su, Y. H., Shen, R. F., Ma, J. F(2024).OsHAK4 functions in retrieving sodium from the phloem at the reproductive stage of rice.Plant J, 120:76-90. [8]陈益芳, 王毅, 武维华(2025).植物钾、磷养分吸收利用机制的研究进展.科学通报, 11:1-13. [9]Gaiss S, Amarasiriwardena D, Alexander D, & Wu, F(2019).Tissue level distribution of toxic and essential elements during the germination stage of corn seeds (Zea mays,L.using LA-ICP-MS.Environ Pollut, 252:657-665. [10]Gupta N, Ram H, Kumar B(2016).Mechanism of Zinc absorption in plants: uptake,transport,translocation and accumulation.Rev Environ Sci Biotechnol, 15:89-109. [11]Hanc A, Baralkiewicz D, Piechalak A, Tomaszewska B, Wagner B, Bulska E(2009).An analysis of long-distance root to leaf transport of lead in Pisum sativum plants by laser ablation–ICP–MS.Int J Environ Anal Chem, 89:651-659. [12]郝艳淑，姜存仓，夏颖，王晓丽，陈防，鲁剑巍(2011).植物钾的吸收及其调控机制研究进展.中国农学通报, 27:6-10. [13]Yang H X, Zhao L H, Gao J X, Liu W, Li B(2014).Bioimaging and Distribution of Cd,P,S,K,Ca,Cu and Zn Elements in Indian Mustard Stem,Chin J Anal Chem 42,355-359.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 42:355-359. [14]Yin J J, Hu X D, Hou Y L, Liu S T, Jia S G, Gan C F, Ou Y L, Zhang XH(2023).Comparative analysis of chemical compositions and antioxidant activities of different pomelo varieties from China.Food Chem Adv, 2:1449-1456. [15]Khan, M.I. R, Nazir F, Maheshwari C., Chopra P, Chhillar H, Sreenivasulu, N(2023).Mineral nutrients in plants under changing environments: A road to future food and nutrition security.The Plant Genome, 16:1-29. [16]Kukusamude C, Kongsri S, Tamklang R, Taebunpakul S(2024).Feasibility of Matrix-Matched Material for Determining Elements in Rice Flour by SN-ICP-MS and LA-ICP-MS.Foods, 13:1-15. [17]赖长鸿，胡逸宸，李培杰，许艺嘉，王浩民，王寒雪，廖红(2024).蜜柚果实养分分配规律及与汁胞品质的关系.中国南方果树, 53:66-72. [18]Limbeck A, Galler P, Bonta M, Bauer G, Nischkauer W, Vanhaecke F(2015).Recent advances in quantitative LA-ICP-MS analysis: challenges and solutions in the life sciences and environmental chemistry.Anal Bioanal Chem, 407:6593-617. [19]龙健梅，郭文武(2018).柑橘石蜡切片.柑橘石蜡切片, 10:1-5. [20]Hennekam R, Jilbert T, Mason P R.D., Lange G J D, Reichart G J(2015).High-resolution line-scan analysis of resin-embedded sediments using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS).Chemical Geology, 403:42-51. [21]Rahman, N F A, Shamsudin R, Ismail A, Shah N N A K, Varith J(2018).Effects of drying methods on total phenolic contents and antioxidant capacity of the pomelo (Citrus grandis (L) Osbeck) peels.Innov Food Sci Emerg Technol, 50:217-225. [22]Sussulini A, Becke J S, Becker J S(2017).Laser ablation ICP-MS: Application in biomedical research.Mass Spec Rev, 1:1-11. [23]Shelden M C, Gilbert S E, Tyerman S D(2020).A laser ablation technique maps differences in elemental composition in roots of two barley cultivars subjected to salinity stress.Plant j, 101:1462-1473. [24]Thyssen G M, Holtkamp M, Kaulfürst S H, Wehe C A, Sperling M, Schaewen V(2017).Elemental bioimaging by means of LA-ICP-OES: investigation of the calcium,sodium and potassium distribution in tobacco plant stems and leaf petioles.Metallomics, 9:676-84. [25]Uchida R(2000).Essential Nutrients for Plant Growth: Nutrient Functions and Deficiency Symptoms.Plant Nutrient Management in Hawaii’s Soils, Approaches for Tropical and Subtropical Agriculture, None:31-55. [26]王衍安(2007).苹果树锌运转分配及缺锌对其生理特性影响的研究.山东农业大学学位论文, None:18-19. [27]Wang Y, Liu Q, Shao Z, Wang X S, Chen Y F, Bai J J, Chen M L, Wang J H(2025)(2025).Investigation on effects of TiO2 on cucumber seedlings using ICP-OES and LA-ICP-MS.Anal Chim Acta, 1352:1-11. [28]White P J, Broadley M R(2003).Calcium in plants.Annals of botany, 92:487-511. [29]Wu, B., Zoriy, M., Chen, Y., Becker, J. S(2009).Imaging of nutrient elements in the leaves of Elsholtzia splendens by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS).Talanta, 78:132-137. [30]Zhang X Y, Geng L P, Gao P P, Dong J W, Zhou C, Li H B, Chen M M, Xue P Y, Liu W J(2022).Bioimaging of Pb by LA-ICP-MS and Pb isotopic compositions reveal distributions and origins of Pb in wheat grain.Sci. Total Environ, 802:1-9. [31]徐世伟, 徐蓉, 孙雨茜, 徐为民, 徐寸发, 王同(2016).电感耦合等离子体质谱法分析菊花茶中微量元素含量及其溶出特性.食品安全质量检测学报, 7:4946-4954. [32]徐浩, 王玉雯, 罗自威, 胡文朗, 廖文强, 陈立松, 李延, 郭九信(2023).养分优化管理提高我国柑橘产量和品质的整合分析.应用生态学报, 35:1301-1311. [33]杨洪强, 接玉玲(2005).植物钙素吸收和运转英文.植物生理与分子生物学学报, 31:227-234. [34]Yamaji N, Ma J F(2019).Bioimaging of multiple elements by high-resolution LA-ICP-MS reveals altered distribution of mineral elements in the nodes of rice mutants.Plant j, 99:1254-1263. [35]Zhu Y B, Jhanis J. Gonzalez, Yang XY, Chan C G Y, He X, Kostecki R, Mao X L, Russo R E, Zorba V(2020).Calcium fluoride as a dominating matrix for quantitative analysis by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS): A feasibility study.Anal Chim Acta, 1129:24-30. [36]叶盛, 汪东风, 丁凌志, 程玉祥(2000).植物体内钙的存在形式研究进展综述.安徽农业大学学报, 27:417-421. [37]Cui Z W, He M, Chen B B, Hu B(2023).In-situ elemental quantitative imaging in plant leaves by LA-ICP-MS with matrix-matching external calibration.Anal Chim Acta, 1275:1-9. [38]赵文志, 张填昊, 张元, 杨园, 卢兵(2023).基于电感耦合等离子体发射光谱法测定植物样品中多元素的前处理方法研究.地质与资源, 32:505-511.

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