基于LA-ICP-MS的文旦柚果实养分原位可视化与半定量分析新方法

doi:10.11983/CBB25113

植物学报 ›› 2026, Vol. 61 ›› Issue (5): 859-870.DOI: 10.11983/CBB25113

基于LA-ICP-MS的文旦柚果实养分原位可视化与半定量分析新方法

王寒雪, 陈昊, 洪佳伟, 陈玥勤, 廖红^*

福建农林大学资源与环境学院, 根系生物学研究中心, 福州 350002

收稿日期:2025-06-19 接受日期:2025-10-11 发布日期:2025-10-22
通讯作者: *廖红, 福建农林大学教授、根系生物学研究中心主任、博士生导师。农业农村部农业科研杰出人才, 国家杰出青年科学基金获得者; 入选国家“百千万人才工程”、国家高层次人才特殊支持计划领军人才、第五批福建省“百人计划”(创新人才)和第三批福建省特支“双百计划”科技创新人才。主持“十三五”、“十四五”国家重点研发计划项目等。主要从事植物营养学、作物养分高效遗传改良和酸性土壤等研究。同时创建了茶树优质高效生态栽培模式, 并入选2021年农业农村部十项绿色发展技术, 在解决科学问题的同时, 促进解决农业农村绿色发展问题。E-mail: hliao@fafu.edu.cn
基金资助:
福建农林大学科技创新专项基金(No.105-KFB24130A)

Visualization and Semi-quantitative Analysis of in-situ Distribution of Nutrients in Wendan Pomelo Fruit Based on LA-ICP-MS

Wang Hanxue, Chen Hao, Hong Jiawei, Chen Yueqin, Liao Hong^*

Root Biology Center, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2025-06-19 Accepted:2025-10-11 Published:2025-10-22
Contact: *E-mail: hliao@fafu.edu.cn

摘要/Abstract

摘要： 原位可视化果实养分浓度, 有助于解析果实养分分配及需求规律, 为果树科学合理施肥提供理论依据。该研究建立了一种基于激光剥蚀-电感耦合等离子体质谱(LA-ICP-MS)原位可视化文旦柚(Citrus maxima)果实养分浓度的新方法。使用石蜡切片法进行前期处理, 避免由于果实柔软多汁等导致组织破损; 使用飞秒激光器, 以研究对象的空间分辨率为主, 通过不同组织离子强度展示各养分的具体分布, 且使用外标法进行基体校正并提供柑橘属中养分元素的相对含量, 原位半定量并可视化分析了文旦柚果实不同部位不同组织的养分浓度。结果表明, 文旦柚果实中, 钾、氮、钙、镁和磷浓度较高, 主要集中于黄皮层的角质层、白皮层的维管束类组织及汁胞的表皮组织。其中, 钙元素相对浓度在黄皮层上最高可达2 302.76 mg∙L^-1, 在整个黄皮层组织平均达到(516.21±28.96) mg∙L^-1, 锌元素相对浓度在黄皮层上最高达到1 388.12 mg∙L^-1, 在整个黄皮层组织平均为(410.22±16.43) mg∙L^-1; 二者同样在表皮细胞中相对浓度最高, 暗示钙元素和锌元素在文旦柚果实中具有重要的营养价值。

关键词: 原位可视化, 激光剥蚀-电感耦合等离子体质谱法, 半定量方法, 养分分布

Abstract: INTRODUCTION: The distribution and concentration of nutrient elements within fruits directly influence their yield and quality. Conventional analytical methods primarily rely on digestion to quantify nutrient content in different fruit tissues. However, as fruits comprise multiple tissue structures, basic quantification techniques fail to precisely resolve nutrient distribution across these microstructural compartments. Consequently, establishing an in-situ method enabling visualization of spatial nutrient concentration profiles in fruit tissues is imperative.
RATIONALE: Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS), as an advanced micro-analytical technique, enables effective visualization of in situ elemental distribution within plant tissues. LA-ICP-MS uses a femtosecond laser (wave-length 343 nm) to scan the fruit sample and the standard sample according to the line of the laser beam during the measurement of the femtosecond laser (wave-length 343 nm). The burned sample material is sent to the ICP with the carrier gas Ar, and the ionic strength of P³¹, K³⁹, Ca⁴⁴, Mg²⁴, Fe⁵⁷, Mn⁵⁵, Cu⁶³, Zn⁶⁶, S³⁴, Al²⁷, Cr⁵², Mo⁹⁵, Pb²⁰⁸, Sr⁸⁸ and Rb⁸⁵ in different parts of the fruit is detected. Visualize the nutrient distribution between different tissues.
RESULTS: Through LA-ICP-MS analysis, we performed in situ, spatially-resolved and semi-quantitative analysis of nutritional element distributions across distinct tissue structures in pomelo fruit during maturation. Significant tissue-specific heterogeneity was observed in elemental distributions among fruit compartments. Three structures demonstrated consistent nutrient enrichment: cuticle of flavedo, vascular bundle-like structures in albedo, and epidermal cells of juice sacs. The concentrations of potassium, nitrogen, calcium, magnesium and phosphorus were higher in the fruit of Wendan pomelo, which were mainly concentrated in the cuticle of flavedo, vascular bundle tissue of albedo and epidermal tissue of juice sac. Among them, the relative average concentration of calcium in the flavedo was as high as (516.21±28.96) mg·L^-1, and the relative average concentration of zinc in the cuticle of the flavedo was (410.22±16.43) mg·L^-1. The relative concentration of the two in the epidermal cells of the juice sac was the highest, suggesting that calcium and zinc play an important nutritional role in the fruit of Wendan pomelo.
CONCLUSION: This study established a novel LA-ICP-MS methodology for in-situ, visual and semi-quantitative analysis for nutrient distribution in different tissue structures of fruits, which was used to establish the spatial relationship of nutrient distribution, filling the gap in the visualization of fruit nutrient distribution. The relative concentration of different elements in each tissue was analyzed, which provided scientific theory for the subsequent guidance of Wendan pomelo farming measures.

Key words: In-situ visualization, laser ablation-inductively coupled plasma mass spectrometry, semi-quantitative method, nutrient distribution

王寒雪, 陈昊, 洪佳伟, 陈玥勤, 廖红. 基于LA-ICP-MS的文旦柚果实养分原位可视化与半定量分析新方法. 植物学报, 2026, 61(5): 859-870.

Wang Hanxue, Chen Hao, Hong Jiawei, Chen Yueqin, Liao Hong. Visualization and Semi-quantitative Analysis of in-situ Distribution of Nutrients in Wendan Pomelo Fruit Based on LA-ICP-MS. Chinese Bulletin of Botany, 2026, 61(5): 859-870.

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https://www.chinbullbotany.com/CN/Y2026/V61/I5/859

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基于LA-ICP-MS的文旦柚果实养分原位可视化与半定量分析新方法

Visualization and Semi-quantitative Analysis of in-situ Distribution of Nutrients in Wendan Pomelo Fruit Based on LA-ICP-MS

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