基于LA-ICP-MS的文旦柚果实养分原位可视化与半定量分析新方法

doi:10.11983/CBB25113

摘要/Abstract

摘要： 原位可视化果实养分浓度, 有助于解析果实养分分配及需求规律, 为果树科学合理施肥提供理论依据。本研究建立了一种基于激光剥蚀-电感耦合等离子体质谱(LA-ICP-MS)、原位可视化文旦柚果实养分浓度的新方法。该方法使用石蜡切片方法进行前期处理避免由于果实柔软、多汁等导致组织破损的问题; 使用飞秒激光器, 以研究对象的空间分辨率为主, 通过不同组织离子强度展示各养分的具体分布, 并且使用外标法进行基体校正并提供柑橘属中养分元素的相对含量, 原位半定量并可视化分析了文旦柚果实不同部位不同组织的养分浓度。结果表明, 文旦柚果实中, 钾、氮、钙、镁和磷浓度较高, 主要集于黄皮层的角质层、白皮层的维管束类组织及汁胞的表皮组织。其中, 钙相对平均浓度在黄皮层高达516.21±387.40 mg∙L^–1, 锌相对平均浓度在黄皮层的角质层上可达410.22±279.77 mg∙L^–1, 二者在汁胞中表皮细胞相对浓度最高, 暗示钙和锌在文旦柚果实中具有重要的营养作用。

关键词: 原位可视化, 激光剥蚀-电感耦合等离子体质谱法, 半定量方法, 养分分布

Abstract: INTRODUCTION: The distribution and concentration of nutrient elements within fruits directly influence their yield and quality. Conventional analytical methods primarily rely on digestion to quantify nutrient content in different fruit tissues. However, as fruits comprise multiple tissue structures, basic quantification techniques fail to precisely resolve nutrient distribution across these microstructural compartments. Consequently, establishing an in-situ method enabling visualization of spatial nutrient concentration profiles in fruit tissues is imperative.
RATIONALE: Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS), as an advanced micro-analytical technique, enables effective visualization of in situ elemental distribution within plant tissues. LA-ICP-MS uses a femtosecond laser (wave-length 343nm) to scan the fruit sample and the standard sample according to the line of the laser beam during the measurement of the femtosecond laser (wave-length 343nm). The burned sample material is sent to the ICP with the carrier gas Ar, and the ionic strength of P³¹, K³⁹, Ca⁴⁴, Mg²⁴, Fe⁵⁷, Mn⁵⁵, Cu⁶³, Zn⁶⁶, S³⁴, Al²⁷, Cr⁵², Mo⁹⁵, Pb²⁰⁸, Sr⁸⁸ and Rb⁸⁵ in different parts of the fruit is detected. Visualize the nutrient distribution between different tissues.
RESULTS: Through LA-ICP-MS analysis, we performed in situ, spatially-resolved and semi-quantitative analysis of nutritional element distributions across distinct tissue structures in pomelo fruit during maturation. Significant tissue-specific heterogeneity was observed in elemental distributions among fruit compartments. Three structures demonstrated consistent nutrient enrichment: cuticle of flavedo, vascular bundle-like structures in albedo, epidermal cells of juice sacs. The concentrations of potassium, nitrogen, calcium, magnesium and phosphorus were higher in the fruit of Wendan pomelo, which were mainly concentrated in the cuticle of flavedo, vascular bundle tissue of albedo and epidermal tissue of juice sac. Among them, the relative average concentration of calcium in the yellow cortex was as high as 516.21 ± 387.40 mg ·L^-1, and the relative average concentration of zinc in the cuticle of the yellow cortex was 410.22 ± 279.77 mg ·L^-1. The relative concentration of the two in the epidermal cells of the juice sac was the highest, suggesting that calcium and zinc play an important nutritional role in the fruit of Wendan pomelo.
CONCLUSION: This study established a novel LA-ICP-MS methodology for in-situ, visual and semi-quantitative analysis for nutrient distribution in different tissue structures of fruits, was used to establish the spatial relationship of nutrient distribution, which filled the gap in the visualization of fruit nutrient distribution. The relative concentration of different elements in each tissue was analyzed, which provided scientific theory for the subsequent guidance of Wendan pomelo farming measures.

LA-ICP-MS in situ visualization and semi-quantitative calcium concentration in various parts of fruit. The in situ distribution of calcium element in the flavedo, albedo and juice sac and the concentration of tissue structure were analyzed.

Key words: In-situ visualization,, laser ablation-inductively coupled plasma mass spectrometry,, semi-quantitative method,, nutrient distribution

王寒雪, 陈昊, 洪佳伟, 陈玥勤, 廖红. 基于LA-ICP-MS的文旦柚果实养分原位可视化与半定量分析新方法. 植物学报, DOI: 10.11983/CBB25113.

Hanxue Wang, Hao Chen, Jiawei Hong, Yueqin Chen, Hong Liao.

Visualization and Semi-quantitative Analysis of in-situ Distribution of Nutrients in Wendan Pomelo Fruit Based on LA-ICP-MS

. Chinese Bulletin of Botany, DOI: 10.11983/CBB25113.

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