荒漠植物红砂AP2/ERF基因家族鉴定及在盐胁迫下的表达模式

朱梦璇; 刘渊; 赵颢宇; 巩薇; 孙振云; 党振华

doi:10.11983/CBB25066

植物学报 >

2026 , Vol. 61 >Issue 3: 449 - 461

DOI: https://doi.org/10.11983/CBB25066

研究论文

荒漠植物红砂AP2/ERF基因家族鉴定及在盐胁迫下的表达模式

朱梦璇 ¹ ,
刘渊 ¹ ,
赵颢宇 ¹ ,
巩薇 ¹ ,
孙振云 ¹ ,
党振华 ^,¹^,^*

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¹ 内蒙古大学生态与环境学院, 蒙古高原生态与资源利用教育部重点实验室, 内蒙古草地生态学重点实验室 , 呼和浩特 010021

^* 党振华, 内蒙古大学生态与环境学院副教授, 博士生导师。主要研究方向为植物分子生态学, 研究内容涉及植物逆境适应进化、植物基因组学及基因功能鉴定等。主持国家自然科学基金、国家重点研发计划项目专题及省级项目8项。在主流学术期刊发表学术论文30余篇。

收稿日期: 2025-04-15

录用日期: 2025-08-20

网络出版日期: 2025-09-03

基金资助

国家自然科学基金(No.32460092)

国家自然科学基金(No.31860078)

内蒙古自然科学基金(No.2024JQ11)

内蒙古大学大学生创新项目()

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Genome-wide Identification of theAP2/ERF Gene Family and Its Expression Patterns Under Salt Stress in the Desert PlantReaumuria soongorica

Mengxuan Zhu ¹ ,
Yuan Liu ¹ ,
Haoyu Zhao ¹ ,
Wei Gong ¹ ,
Zhenyun Sun ¹ ,
Zhenhua Dang ^,¹^,^*

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¹ Inner Mongolia Key Laboratory of Grassland Ecology, Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau, School of Ecology and Environment, Inner Mongolia University , Hohhot 010021, China

Received date: 2025-04-15

Accepted date: 2025-08-20

Online published: 2025-09-03

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摘要

红砂 ( Reaumuria soongorica) 是我国西北荒漠区广泛分布的耐盐旱生小灌木, 具有极强的生境适应性, 是研究植物抗逆分子机制的理想材料。以红砂基因组为参考, 鉴定其 AP2/ ERF基因家族成员, 并分析其系统发育、基因结构、保守基序、顺式作用元件、基因复制事件以及该家族成员在盐胁迫下的表达模式。结果表明, 红砂基因组中有70个 AP2/ ERF基因, 系统发育分析显示其可分为 AP2、 ERF、 DREB和 RAV亚家族; 其启动子区含有多种光响应、胁迫响应、生长发育和激素响应等顺式作用元件。红砂 AP2/ ERF基因非均匀分布在其基因组的11条染色体上, 其中63个基因由不同基因复制事件演化而来, 全基因组复制和分散复制是该基因家族扩张的主导因素。在盐胁迫下, 红砂 AP2/ ERF基因呈现不同的表达模式, 6个基因显著差异表达, 可能在红砂响应盐胁迫过程中发挥积极作用。该研究从基因组水平鉴定和分析了红砂 AP2/ ERF基因家族的特征, 旨在为进一步解析其在逆境适应中的分子功能奠定基础。

关键词： AP2/ ERF基因家族; 基因结构分析; 盐胁迫; 基因表达; 红砂

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朱梦璇 , 刘渊 , 赵颢宇 , 巩薇 , 孙振云 , 党振华 . 荒漠植物红砂AP2/ERF基因家族鉴定及在盐胁迫下的表达模式[J]. 植物学报, 2026 , 61(3) : 449 -461 . DOI: 10.11983/CBB25066

Abstract

INTRODUCTION: Reaumuria soongorica, a perennial small shrub, is widely distributed in arid desert regions of northwestern China. It exhibits exceptional drought and salt tolerance, making it an ideal model for studying molecular mechanisms of plant stress resistance. RATIONALE: We used the R. soongorica genome as a reference to identify members of the AP2/ ERF gene family in this species, and analyzed their phylogeny, gene structure, conserved motifs, cis-acting elements, gene duplication events, as well as the expression patterns of these family members under salt stress. RESULTS: Seventy AP2/ ERF genes were identified from the R. soongorica genome. Phylogenetic analysis classified these genes into four subfamilies: AP2, ERF, DREB, and RAV. Cis-acting element analysis revealed multiple regulatory elements associated with light responsiveness, stress adaptation, growth regulation, and hormone signaling in the promoter regions of R. soongorica AP2/ ERF genes. These genes exhibited an uneven distribution across all 11 chromosomes of the R. soongorica genome, with 63 genes (90% of the total) originating from gene duplication events. Evolutionary analysis suggested that whole-genome duplication (WGD) and dispersed duplication were the primary drivers of family expansion. Under salt stress, AP2/ ERF genes showed divergent expression patterns in R. soongorica seedlings, with six genes displaying significant differential expression (|log ₂FC|≥1, P<0.05), implicating their potential roles in salt stress response. CONCLUSION: This study identified and characterized the AP2/ ERF gene family in R. soongorica at the genomic level, thereby establishing a foundation for elucidating its functional roles in this species’ adaptation to arid and saline environments.

Key words： AP2/ ERF gene family; gene structure analysis; salt stress; gene expression; Reaumuria soongorica

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