教学研讨

CRISPR/Cas9技术在水稻育种中的基因编辑实验教学设计

  • 吕若彤 ,
  • 寇云霞 ,
  • 肖雨佳 ,
  • 艾鹏飞 ,
  • 王雁伟
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  • 河北科技大学食品与生物学院, 石家庄 050018

收稿日期: 2025-04-27

  修回日期: 2025-07-18

  网络出版日期: 2025-07-30

基金资助

河北省高等学校科学研究项目(No.BJK2024165)、河北省教育科学“十四五规划青年专项课题(No.2406039)、河北科技大学引进人才科研启动基金(No.QD2023010)和河北省教育厅在读研究生创新能力培养资助项目(No.CXZZSS2025067)

Experimental Teaching Design for Gene Editing in Rice Breeding by CRISPR/Cas9 Technology

  • LV Re-Tong ,
  • KOU Yun-Xia ,
  • XIAO Yu-Jia ,
  • YI Peng-Fei ,
  • YU Yan-Wei
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  • College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China

Received date: 2025-04-27

  Revised date: 2025-07-18

  Online published: 2025-07-30

摘要

CRISPR/Cas9技术作为一种高效的基因组编辑工具, 在生命科学领域的应用已经变得越来越广泛。将这一技术引入大学的实验教学中, 能够帮助学生更深入地理解前沿科研进展, 增强科研创新实践能力。本文设计了一个创新性教学实验, “利用CRISPR/Cas9技术研究敲除水稻SSA1 (seedling stage albino1)基因对表型的影响”。该实验旨在帮助学生加深理解CRISPR/Cas9技术的原理和实验流程, 直观认识到SSA1基因在水稻生长发育中发挥的重要作用的同时, 提高学生实验操作的能力和严谨的科研思维, 培养学生的探索能力和创新精神。

本文引用格式

吕若彤 , 寇云霞 , 肖雨佳 , 艾鹏飞 , 王雁伟 . CRISPR/Cas9技术在水稻育种中的基因编辑实验教学设计[J]. 植物学报, 0 : 1 -0 . DOI: 10.11983/CBB25080

Abstract

CRISPR/Cas9 technology, as an efficient genome editing tool, has gained increasingly widespread application in the life sciences. Incorporating this technology into university experimental teaching enables students to deepen their understanding of cutting-edge research advances while enhancing their innovative research capabilities and practical skills. In this paper, we have designed an innovative teaching experiment, “Using CRISPR/Cas9 technology to study the effect of knocking out rice SSA1(seedling stage albino1)gene on phenotype”. This experiment helps students to deepen their understanding of the principles and experimental procedures of CRISPR/Cas9 technology and to recognize the important role played by theSSA1 gene in rice growth and development. At the same time, the experiment can improve students' ability of experimental operation and rigorous scientific research thinking, and cultivate students' exploratory skills and innovative spirit.


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