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DOI: https://doi.org/10.11983/CBB24191

研究论文

不同钾水平下烟草根内皮层木栓化的适应性发育差异研究

  • 许耘祥 ,
  • 张莉汶 ,
  • 顾迎晨 ,
  • 张标 ,
  • 朱莹莹 ,
  • 刘海伟
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  • 1中国农业科学院烟草研究所/农业农村部烟草生物学与加工重点实验室, 山东青岛 266101; 2中国农业科学院研究生院, 北京 100081


收稿日期: 2024-12-09

  修回日期: 2025-03-04

  网络出版日期: 2025-03-18

基金资助

山东省自然科学基金面上项目(No.ZR2023MC150)和中国农业科学院科技创新工程项目(No.ASTIP-TRIC03)

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Differences in Adaptive Development of Suberin in Tobacco Root Endothelial Layer under Different Potassium Levels

  • XU Yun-Xiang ,
  • ZHANG Li-Men ,
  • GU Ying-Chen ,
  • ZHANG Biao ,
  • ZHU Ying-Ying ,
  • LIU Hai-Wei
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  • 1Institute of Tobacco Research of CAAS/Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Qingdao 266101, China; 2Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2024-12-09

  Revised date: 2025-03-04

  Online published: 2025-03-18

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摘要

根内皮层分化的质外体屏障在植物抗逆和养分吸收均起到重要作用, 其中木栓层发育成为近年研究的热点。本研究以烟草栽培品种中烟100为材料, 通过0.1–4.0 mmol∙L–1钾浓度梯度水培实验, 探讨供钾水平对根内皮层木栓化发育的影响及其生理分子机制。结果表明，低钾胁迫(0.1 mmol∙L–1)显著增强内皮层木栓化: 完全木栓化区域绝对长度由对照的0–2 cm延伸至4–6 cm, 相对占比从0–15.0%提升至33.2–44.3%, 表明木栓化是烟草响应低钾胁迫的关键形态适应机制之一。表型分析显示, 低钾胁迫下植株根系伸长但生物量下降, 地上部与根系钾离子含量及积累量分别减少, 木质部汁液流量及钾离子浓度运输效率降低。内源激素含量检测发现, 低钾胁迫提高根系内源脱落酸含量, 并抑制乙烯和茉莉酸甲酯含量, 形成特异性激素调控网络。转录组数据进一步佐证木栓化发育的分子基础, 木栓质合成与转运相关基因(如CYP86、GPAT、ABCG)及其上游正调控因子MYB36/41/92/93显著上调。因此, 本研究首次阐明烟草低钾胁迫通过ABA介导的激素信号, 调控木栓化发育程序, 为解析作物钾胁迫适应机制提供了全新视角。

关键词： 木栓化; 钾; 根内皮层; 激素

本文引用格式

许耘祥 , 张莉汶 , 顾迎晨 , 张标 , 朱莹莹 , 刘海伟 . 不同钾水平下烟草根内皮层木栓化的适应性发育差异研究[J]. 植物学报, 0 : 1 -0 . DOI: 10.11983/CBB24191

Abstract

INTRODUCTION: The apoplastic barriers differentiated from the root endodermis play an important role in plant stress resistance and nutrient uptake, and the development of suberin lamella has become a hot research topic in recent years.



RATIONALE: Hydroponic experiments with potassium concentration gradients ranging from 0.1 to 4.0 mmol∙L–1 were conducted, using the cultivated tobacco variety Zhongyan 100 as the experimental material, to explore the effect of different potassium supplies on the endodermal suberization and its physiological and molecular mechanisms.


RESULTS: It was found that low potassium stress (0.1 mmol∙L–1) significantly enhanced the endodermal suberization: the absolute length of the fully suberized region extended from 0–2 cm in the control to 4–6 cm, and the relative proportion increased from 0–15.0% to 33.2–44.3%. This indicates that suberization is one of the key morphological adaptation mechanisms in tobacco under low potassium stress. Phenotypic analysis showed that under low potassium stress, root elongation increased while plant biomass decreased, and potassium ion content and accumulation in both the aboveground and root parts were reduced. Additionally, the flow rate and potassium ion concentration in the xylem sap decreased, indicating reduced transport efficiency. Endogenous hormone analysis revealed that low potassium stress increased abscisic acid (ABA) content in roots while suppressing ethylene and methyl jasmonate levels, forming a specific hormonal regulatory network. Transcriptome data further supported the molecular basis of suberization development, showing significant upregulation of genes related to suberin synthesis and transport (e.g., CYP86, GPAT, ABCG) and their upstream positive regulatory factors (MYB36, MYB41, MYB92, MYB93).


CONCLUSION: This study is the first to elucidate that low potassium stress regulates the suberization developmental program of tobacco root through ABA-mediated hormonal signaling reprogramming, providing a novel perspective for understanding the adaptation mechanisms of plants to potassium stress.





Fluorescence imaging of transverse sections of endodermis development in tobacco root under three potassium concentrations



Key words： suberization,; potassium,; root endodermis,; hormone

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参考文献

[1]郭泽, 李子绅, 代晓燕, 王英锋(2019).低钾胁迫下外源生长素对烟草根系生长及钾吸收的影响.植物营养与肥料学报, 25:1173-1184. [2]罗海斌, 黄诚梅, 曹辉庆, 蒋胜理, 吴兴剑, 叶丽萍, 魏源文(2022).不同浓度钾元素对西番莲组培苗根系生长和内源激素含量的影响., 中国果树:53-58. [3]王立梅, 刘奕清, 阮玉娟 (2015).植物钾素研究进展..中国园艺文摘, 31:71+148.-. [4]闫慧峰, 石屹, 李乃会, 张永春(2013).烟草钾素营养研究进展.中国农业科技导报, 15:123-129. [5]张标, 吴健, 张杨, 董小卫, 韩硕, 高昕, 杜从伍, 李慧英, 种学法, 朱莹莹, 刘海伟(2023).木栓层在水和溶质运输中的生理功能研究进展.植物学报, 58:1008-1018. [6]张标, 许耘祥, 张莉汶, 朱莹莹, 刘海伟(2024).低-胁迫抑制烟草根系质外体运输的机制研究.中国烟草科学, 45:25-34. [7]Andersen T G, Barberon M, Geldner N(2015).Suberization - the second life of an endodermal cell.Curr Opin Plant Biol, 28:9-15. [8]Barberon M(2017).The endodermis as a checkpoint for nutrients.New Phytol, 213:1604-1610. [9]Barberon M, Vermeer JE, De Bellis D, Wang P, Naseer S, Andersen TG, Humbel BM, Nawrath C, Takano J, Salt DE, Geldner N(2016).Adaptation of Root Function by Nutrient-Induced Plasticity of Endodermal Differentiation.Cell, 164:447-459. [10]Baxter I, Hosmani PS, Rus A, Lahner B, Borevitz JO, Muthukumar B, Mickelbart MV, Schreiber L, Franke RB, Salt DE (2009).Root suberin forms an extracellular barrier that affects water relations and mineral nutrition in Arabidopsis. PLoS Genet, 5, e1000492.., :-. [11]Chen A, Liu T, Deng Y, Xiao R, Zhang T, Wang Y, Yang Y, Lakshmanan P, Shi X, Zhang F, Chen X (2023).Nitrate_dependent suberization regulates cadmium uptake and accumulation in maize. Science of The Total Environment, 878, 162848.., :-. [12]Leide J, Hildebrandt U, Hartung W, Riederer M, Vogg G(2012).Abscisic acid mediates the formation of a suberized stem scar tissue in tomato fruits.New Phytologist, 194:402-415. [13]Liu Y, Lu M, Persson DP, Luo J, Liang Y, Li T (2022).The involvement of nitric oxide and ethylene on the formation of endodermal barriers in response to Cd in hyperaccumulator Sedum alfredii. Environ Pollut, 307, 119530.., :-. [14]Liu Y, Tao Q, Li J, Guo X, Luo J, Jupa R, Liang Y, Li T (2021).Ethylene-mediated apoplastic barriers development involved in cadmium accumulation in root of hyperaccumulator Sedum alfredii. J Hazard Mater, 403, 123729.., :-. [15]Lulai EC, Suttle JC, Pederson SM(2008).Regulatory involvement of abscisic acid in potato tuber wound-healing.Journal of Experimental Botany, 59:1175-1186. [16]Lux A, Morita S, Abe J, Ito K(2005).An improved method for clearing and staining free-hand sections and whole-mount samples.Ann Bot, 96:989-996. [17]Melino VJ, Plett DC, Bendre P, Thomsen HC, Zeisler-Diehl VV, Schreiber L, Kronzucker HJ (2021).Nitrogen depletion enhances endodermal suberization without restricting transporter-mediated root NO3(-) influx. J Plant Physiol, 257, 153334.., :-. [18]Naseer S, Lee Y, Lapierre C, Franke R, Nawrath C, Geldner N 2012.Casparian strip diffusion barrier in Arabidopsis is made of a lignin polymer without suberin. Proc Natl Acad Sci USA, 109, 10101-10106.., :-. [19]Pfister A, Barberon M, Alassimone J, Kalmbach L, Lee Y, Vermeer J E, Yamazaki M, Li G, Maurel C, Takano J, Kamiya T, Salt D E, Roppolo D, Geldner N (2014).A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects. eLife, 3, e03115.., :-. [20]Tao Q, Jupa R, Liu Y, Luo J, Li J, Kovac J, Li B, Li Q, Wu K, Liang Y, Lux A, Wang C, Li T(2019).Abscisic acid-mediated modifications of radial apoplastic transport pathway play a key role in cadmium uptake in hyperaccumulator Sedum alfredii.Plant Cell Environ, 42:1425-1440. [21]Tao Q, Li M, Xu Q, Kovac J, Yuan S, Li B, Li Q, Huang R, Gao X, Wang C (2022).Radial transport difference mediated by root endodermal barriers contributes to differential cadmium accumulation between japonica and indica subspecies of rice (Oryza sativa L.). J Hazard Mater, 425, 128008.., :-. [22]Vestenaa MW, Husted S, Minutello F, Persson DP (2024).Endodermal suberin restricts root leakage of cesium: a suitable tracer for potassium. Physiologia Plantarum, 176.., :-. [23]Wang P, Wang C-M, Gao L, Cui YN, Yang HL, de Silva N D G, Ma Q, Bao AK, Flowers TJ, Rowland O, Wang SM(2020).Aliphatic suberin confers salt tolerance to Arabidopsis by limiting Na+ influx,K+ efflux and water backflow.Plant and Soil, 448:603-620. [24]Wei X, Liu L, Jin X, Xue J, Geng P, Xu Z, Zhang L, Wang X, Zong W, Zhang L, Mao L (2024a).Exogenous methyl jasmonate promotes wound healing of Chinese yam tubers (Dioscorea opposita) through the deposition of suberin polyaliphatics at the wound sites. Postharvest Biology and Technology, 207.., :-. [25]Wei X, Liu L, Liu G, Geng P, Wei X, Yao X, Chen J, Gong W, Ge Z, Liu M, Mao L (2024b.) Methyl jasmonate promotes suberin biosynthesis by stimulating transcriptional activation of AchMYC2 on AchFHT in wound healing of kiwifruit. Postharvest Biology and Technology, 210.., :-. [26]Wei X, Liu L, Xu Z, Xue J, Geng P, Ge Z, Wang X, Zhang L, Zong W, Mao L (2023).Methyl jasmonate facilitates wound healing of Chinese yam tubers via positively regulating the biosynthesis and polymerization of suberin polyphenolics. Scientia Horticulturae, 312.., :-. [27]Zhang B, Xu Y, Zhang L, Yu S, Zhu Y, Liu C, Wang P, Shi Y, Li L, Liu H (2024).Root endodermal suberization induced by nitrate stress regulate apoplastic pathway rather than nitrate uptake in tobacco (Nicotiana tabacum L.). Plant Physiology and Biochemistry, 216, 109166.?., :-.
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