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研究报告

拟南芥AtFTCD-L参与根系响应土壤紧实度的机制研究

  • 刘雨函 ,
  • 曹启江 ,
  • 张诗晗 ,
  • 李益慧 ,
  • 王菁 ,
  • 谭晓萌 ,
  • 刘筱儒 ,
  • 王显玲
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  • 1沈阳大学生命科学与工程学院, 辽宁省城市有害生物治理与生态安全重点实验室, 沈阳 110044; 2沈阳农业大学生物科学技术学院, 沈阳 110866


收稿日期: 2024-10-13

  修回日期: 2025-01-14

  网络出版日期: 2025-01-22

基金资助

2024年辽宁省教育厅基本科研项目(No.LJ212411035019)、2024年沈阳大学本科大学生创新创业训练计划项目-国家级(No.202411035017)

Mechanism of AtFTCD-L in Root Response to Soil Compaction

  • LIU Yu-Han ,
  • CAO Qi-Jiang ,
  • ZHANG Shi-Han ,
  • LI Yi-Hui ,
  • YU Jing ,
  • TAN Xiao-Meng ,
  • LIU Xiao-Ru ,
  • YU Xian-Ling
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  • 1Key Laboratory of Urban Pest Control and Ecological Security in Liaoning, College of Life Sciences and Engineering, Shenyang University, Shen Yang 110044, China; 2College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China

Received date: 2024-10-13

  Revised date: 2025-01-14

  Online published: 2025-01-22

摘要

植物根系在生长发育过程中响应各种非生物胁迫, 包括干旱胁迫、重金属胁迫、盐胁迫、冷热胁迫以及生理性缺素等, 其中土壤结构特别是土壤紧实度会影响根系的生长与形态, 进而影响作物的产量。高尔基体通过囊泡分泌参与根系的生长以及响应非生物胁迫。但是, 高尔基体如何参与根系响应土壤紧实度的机制还不清楚。前期研究发现拟南芥中AtFTCD-L定位在高尔基体反面(trans Golgi network, TGN)上, 参与囊泡的分选和/或分泌调节根冠外周细胞中黏液成分。本文作者在前期研究基础上, 模拟土壤高紧实度生长实验, 观察稳定表达PINs-GFP的纯合体植株表型, 通过生长素相关荧光信号的观察, 发现AtFTCD-L突变体根尖以及根尖细胞在纵向上短于野生型等材料, 而在横向上宽于野生型等材料, 并且细胞形态异常明显。通过对PINs相关材料进行荧光信号收集, 发现突变体植株中PIN7低表达或不表达。综上表明, AtFTCD-L在拟南芥植株根系中通过调节PIN7的分布或表达来响应土壤紧实度。本研究为揭示植物根系响应土壤紧实度非生物胁迫的适应机制提供一定理论指导。

本文引用格式

刘雨函 , 曹启江 , 张诗晗 , 李益慧 , 王菁 , 谭晓萌 , 刘筱儒 , 王显玲 . 拟南芥AtFTCD-L参与根系响应土壤紧实度的机制研究[J]. 植物学报, 0 : 1 -0 . DOI: 10.11983/CBB24154

Abstract

Plant roots respond to various abiotic stresses during their growth and development, including drought stress, heavy metals stress, salt stress, and deficiencies in essential nutrients. Among these factors, soil structure, especially soil compaction significantly affects root growth and morphology, ultimately influencing crop yield. The Golgi apparatus plays a role in root growth and responds to abiotic stress through vesicle secretion. However, the mechanisms by which the Golgi apparatus contributes to the root system's response to soil compaction remain unclear. Previous studies have demonstrated that AtFTCD-L in Arabidopsis is located on the trans-Golgi network (TGN) opposite the Golgi apparatus, and plays a role in vesicle sorting and/or secretion regulation of mucin components in the peripheral cells of the root cap. Based on previous research, this paper simulates growth experiments under conditions of high soil compaction to observes the phenotype of stable expression of the PIN-FORMED(PIN) GFP homozygous, and collects auxin-related fluorescence signals. The findings indicate that mutant root tips and root tip cells are shorter in the longitudinal direction compared to wild-type, but wider in the transverse direction, exhibiting significantly abnormal cell morphology. Analysis of fluorescent signals from PIN-related materials revealed that PIN7 was either not expressed or expressed at very low levels in mutant plants. In summary, AtFTCD-L responds to soil compaction in the roots of Arabidopsis by regulating the distribution or expression of PIN7. This study provides theoretical insights into the adaptive mechanisms of plant roots in response to abiotic stress induced by soil compaction.
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