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植物学报 >

2025 , Vol. 60 >Issue 3: 377 - 392

DOI: https://doi.org/10.11983/CBB24117

研究论文

菠菜AT-hook基因家族鉴定与表达谱分析

  • 杨莉 ,
  • 曲茜彤 ,
  • 陈子航 ,
  • 邹婷婷 ,
  • 王全华 ,
  • 王小丽
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  • 上海师范大学生命科学学院/植物种质资源开发协同创新中心, 上海 200234
*王小丽, 上海师范大学生命科学学院副教授, 硕士生导师。主要从事园艺植物营养品质调控、遗传鉴定及新品种选育等工作。目前研究团队以菠菜为主要研究对象, 收集了世界各地菠菜种质资源千余份, 完成菠菜资源的深度转录组测序和全基因组测序工作, 利用生理生化、遗传学和分子生物学等手段对其重要农艺性状进行研究。E-mail: wangxl@shnu.edu.cn

收稿日期: 2024-07-30

  录用日期: 2024-12-26

  网络出版日期: 2024-12-27

基金资助

上海市科技兴农项目(2022-02-08-00-12-F01116)

收起

Identification of the Spinach AT-hook Gene Family and Analysis of Expression Profiles

  • Yang Li ,
  • Qu Xitong ,
  • Chen Zihang ,
  • Zou Tingting ,
  • Wang Quanhua ,
  • Wang Xiaoli
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  • Development and Collaborative Innovation Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China

Received date: 2024-07-30

  Accepted date: 2024-12-26

  Online published: 2024-12-27

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摘要

AHL (AT-hook)蛋白家族在植物生长发育、器官构建、逆境胁迫及植物激素信号应答中发挥关键作用。为揭示菠菜(Spinacia oleracea) AHL基因家族的生物学功能, 在基因组范围内对SoAHL家族成员进行鉴定和分析, 并探究外源水杨酸对其表达水平的影响。结果表明, SoAHL家族包含19个成员, 在6条染色体间不均匀分布, 系统发育树将其分为2个亚族, I、II亚族分别包含10、9个SoAHL成员; 不同亚族间PPC和AT-hook保守基序序列组成有差异; SoAHL主要定位在细胞核、细胞质和线粒体; SoAHL I亚族成员无内含子, II亚族成员含4-5个内含子; SoAHL启动子上游分布有不同数量的植物激素和非生物胁迫响应相关顺式作用元件。SoAHL成员在根、叶片及叶柄中均有表达, 大多数在根中表达水平较高。水杨酸处理条件下, SOV6g041850.1和SOV2g038950.1受水杨酸显著诱导; SOV2g031340.1和SOV4g018880.1主要在地上部表达, 与叶酸含量的组织分布特征相似。瞬时过表达SOV4g018880.1能提高菠菜叶酸含量1.75倍。研究结果可为进一步解析SoAHL的功能奠定基础。

关键词： 菠菜; AT-hook基因家族; 生物信息学; 水杨酸

本文引用格式

杨莉 , 曲茜彤 , 陈子航 , 邹婷婷 , 王全华 , 王小丽 . 菠菜AT-hook基因家族鉴定与表达谱分析[J]. 植物学报, 2025 , 60(3) : 377 -392 . DOI: 10.11983/CBB24117

Abstract

INTRODUCTION: The AT-hook motif nuclear localized (AHL) gene family is a highly conserved transcription factors involved in plant growth, development, and stress responses, but their roles in spinach are still unknown.

RATIONALE:To reveal the basic characteristics of the AHL family in spinach, members of the spinach SoAHLfamily were identified at the whole-genome level, and their physicochemical properties, gene structure, conserved motifs, promoter elements, and salicylic acid-responsive expression profiles were analyzed in this study.

RESULTS: The results revealed 19 SoAHL family members in the spinach genome, which were unevenly distributed across six chromosomes. These SoAHL members can be classified into three branches, with 10 members in subfamily I and 9 members in subfamily II. The sequence composition of PPC and AT-hook conserved motifs varies among subfamilies; most of the SoAHL genes are located in the nucleus, cytoplasm, and mitochondrion. Members of subfamily I of SoAHL have no introns, whereas members of subfamily II contain 4-5 introns. The varying numbers of cis-acting elements relate to phytohormones and abiotic stress responses were distributed upstream of the promoters of the SoAHL members. The SoAHL genes can be expressed in roots, leaves, and petioles, with most genes expressed at relatively high levels in roots. The expression of two SoAHL genes (SOV6g041850.1 and SOV2g038950.1) was significantly induced by salicylic acid treatment. The expression profiles and salicylic acid-induced expression levels of SOV2g031340.1 and SOV4g018880.1 were highly correlated with the folic acid content, which may play a role in the spinach response to the salicylic acid signaling pathway. The transient overexpression of SOV4g018880.1 increased the folate content of spinach leaves by 1.75 times.

CONCLUSION: The results from the sequence characteristics, expression profiles and exogenous salicylic acid treatment revealed that the SoAHLs had potential functional diversity and that specific members may have positive effects on spinach folate accumulation. Our results will lay the foundation for further resolving the function of spinach AT-hook genes.



Phenotype (A), total folate content (B) and expression analysis of SoAHL (C) under 50 μmol∙L-1 salicylic acid (SA) treatment for 5 days (D5) and 7 days (D7). CK: Control

Key words： spinach; AT-hook gene family; bioinformatics; salicylic acid

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