紫薇GolS家族基因理化特性与表达特征分析

徐田甜; 曹怡; 杨培建; 杨帆; 周晓茜; 陈艳红; 魏辉; 张健

doi:10.11983/CBB24118

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DOI: https://doi.org/10.11983/CBB24118

紫薇GolS家族基因理化特性与表达特征分析

徐田甜 ,
曹怡 ,
杨培建 ,
杨帆 ,
周晓茜 ,
陈艳红 ,
魏辉 ,
张健

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¹南通大学生命科学学院, 南通 226019; ²南通市观赏植物遗传育种重点实验室, 南通 226019

收稿日期: 2024-08-04

修回日期: 2024-11-12

网络出版日期: 2024-11-26

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Analysis of physicochemical characteristics and expression characteristics of lagerstroemia GolS family genes

XU Tian-Tian ,
CAO Yi ,
YANG Pei-Jian ,
YANG Fan ,
ZHOU Xiao-Qian ,
CHEN Yan-Hong ,
WEI Hui ,
ZHANG Jian

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¹School of Life Sciences, Nantong University, Nantong 226019, China; ²Nantong Key Laboratory of Ornamental Plant Genetics and Breeding, Nantong 226019, China

Received date: 2024-08-04

Revised date: 2024-11-12

Online published: 2024-11-26

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摘要

植物肌醇半乳糖苷合成酶(galactinol synthase, GolS) 是棉子糖家族寡糖(raffinose family oligosaccharides, RFOs) 生物合成途径中的关键酶, 为棉子糖系列寡糖提供活化的半乳糖基, 调控植物体内棉子糖系列寡糖的生物合成与积累, 在植物对非生物胁迫的反应中发挥重要作用。然而, 关于紫薇(Lagerstroemia indica) GolS (LiGolS)基因成员的分子结构特征还未见研究报道。该研究在全基因组水平上鉴定了13个紫薇LiGolS基因成员, 并对其理化性质、染色体定位、进化关系、基因结构、保守基序以及盐胁迫下的表达量进行了分析。结果表明: 13个LiGolS基因不均匀地分布在10条染色体上, 13个LiGolS蛋白的等电点为4.75−9.45, 分子量变化范围为37.69−46.12 kDa, 氨基酸数量为327−404 aa; 亚细胞定位预测结果发现6个蛋白定位在叶绿体上, 1个蛋白定位在线粒体, 5个蛋白定位在细胞质, 1个定位在液泡。13个基因成员含有的外显子数目为0−4。基于盐胁迫LiGolS的表达分析表明, 盐处理后所有LiGolS基因成员表现出不同程度的上调表达, 表明这些基因可能参与了紫薇的盐胁迫响应。本研究结果为后续开展紫薇GolS基因的功能解析奠定了基础。

关键词： 紫薇; GolS基因家族; 盐胁迫

本文引用格式

徐田甜 , 曹怡 , 杨培建 , 杨帆 , 周晓茜 , 陈艳红 , 魏辉 , 张健 . 紫薇GolS家族基因理化特性与表达特征分析[J]. 植物学报, 0 : 1 -0 . DOI: 10.11983/CBB24118

Abstract

galactinol synthase (GolS) is a key enzyme in the biosynthesis pathway of raffinose family oligosaccharides (RFOs), providing activated galactosyl for raffinose family oligosaccharides. Regulation of biosynthesis and accumulation of raffinose oligosaccharides in plants plays an important role in plant response to abiotic stress. However, the molecular structure characteristics of GolS gene family members of Lagerstroemia indica have not been reported. In this study, 13 members of the LiGolS gene family were identified at the whole genome level, and their physicochemical properties, chromosome localization, evolutionary relationship, gene structure, conserved motifs, and expression under salt stress were analyzed. The results showed that the 13 LiGolS genes were unevenly distributed on 10 chromosomes. The isoelectric point of the 13 LiGolS proteins was 4.75−9.45, the molecular weight was 37.69−46.12 kDa, and the amino acid number was 327−404 aa. Subcellular localization prediction results showed that 9 proteins were located in the nucleus, 6 proteins were located in the chloroplast, 1 protein was located in the mitochondria, 5 proteins were located in the cytoplasm, and 1 protein was located in the vacuole. Thirteen gene members contain exons with a number of 0–4. Analysis of LiGolS expression based on salt stress showed that all LiGolS gene members showed varying degrees of up-regulated expression after salt treatment, indicating that these genes may be involved in the salt stress response of Crape Myrtle. The results of this study laid a foundation for further functional analysis of LiGolS gene.

Key words： Crape Myrtle; GolS gene family; Salt stress

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