Abstract: The rate of virus-free bulblets obtained by adventitious bud culture depended on cultivar of Lilium spp. and virus kind. Lily symptomless virus (LSV) was not detected in 76%of L.בCasablanca’; all bulblets of L.בEnchantment’were Cucumber mosaic virus (CMV) free ones. Adventitious bud of L.בCasablanca’, which were excised from scales that had been cultured on a solid medium for 3～4 weeks,were cultured in liquid medium or on solid one. All explants developed bulblets on both medium, although the fresh weight of bulblets was highest in those cultured in a liquid medium supplemented with antiviral chemical (10 mg/L DHT); the rate of virus-free bulblets was highest, when cultured in the media containing 10 mg/L DHT. Bulblets obtained by adventitious bud culture,in which virus had not been detected by ELISA, were transplanted to pots and cultivated in insect-proof condition. Two years later, the virus-detection test was employed for these plants. The rate of plants infected with viruses depended on Lilium spp.; Viruses were detected in 73% of L.בCasablanca’and 17% of L. longilorum ‘Georgia’. In L.longilorum‘Georgia’,L.nobilissimum and L.בCasablanca’, the height of the virus-free plants was higher than that of infected plants.