植物学报 ›› 1989, Vol. 6 ›› Issue (02): 124-126.

• 技术与方法 • 上一篇    下一篇

绒毡层及小孢子发育研究的扫描电镜样品制备

陈宝理 金晓萍 张起麟   

  1. 1(华中农业大学,武汉) 2(武汉大学,武汉)
  • 出版日期:1989-06-20 发布日期:1989-06-20

Specimen Preparation of Scanning Eletron Microscopy for Investigation on Development of Tapetum and Microspore

Chen Bao-li, Jin Xiao-ping and Zhang Qi-li   

  • Online:1989-06-20 Published:1989-06-20

摘要: 锇酸-二甲基亚砜一锇酸冷冻割断法制备的样品,在扫描电镜下,可观察绒毡层及小孢子发育过程,细胞形态保存良好,结构清晰,立体感强,为用扫描电镜研究植物细胞结构提供了一个有应用价值的方法。

Abstract: The development of tapetum, microscope and stereoscopic structure of the whole cell were clearly observed in a stamen specimen prepared with osmium-DNSO-osmium freeze cracking method. The results indicated that it is a good method and can be applied on studying cell structure of plant specimen with scanning electron microscopy.