植物学报 ›› 2005, Vol. 22 ›› Issue (01): 27-31.
• 研究论文 • 上一篇 下一篇
张国增 安国勇 宋纯鹏
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ZHANG Guo-Zeng AN Guo-Yong SONG Chun-Peng
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摘要: 本文以沙培养法、蛭石培养法、土培养法、水培养法和MS培养基等不同的方法培养拟南芥(Arabidopsis thaliana),分析了不同培养方法对根生长发育的影响,并分别分离根的原生质体。在膜片钳记录中对不同来源的根原生质体状态进行了比较。结果表明,土培养法分离的原生质体最适于膜片钳记录。
Abstract: We analyzed the effects of different cultural conditions — sand, vermiculite, nutritive soil, water and Murashige-Skoog medium — on the development of root protoplasts isolated from Arabidopsis thaliana. Patch clamp recording revealed that the quality of root protoplasts was superior with nutritive soil culture.
张国增 安国勇 宋纯鹏. 不同培养条件对拟南芥根细胞膜片钳记录的影响. 植物学报, 2005, 22(01): 27-31.
ZHANG Guo-Zeng AN Guo-Yong SONG Chun-Peng. Patch Clamp Recording of Arabidopsis Root Cells Under Different Cultural Conditions. Chinese Bulletin of Botany, 2005, 22(01): 27-31.
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