番茄环纹斑点病毒与马铃薯Y病毒复合侵染烟草的细胞病理特征

doi:10.3724/SP.J.1259.2014.00704

植物学报 ›› 2014, Vol. 49 ›› Issue (6): 704-709.DOI: 10.3724/SP.J.1259.2014.00704 cstr: 32102.14.SP.J.1259.2014.00704

番茄环纹斑点病毒与马铃薯Y病毒复合侵染烟草的细胞病理特征

方琦^†, 董家红^†, 郑宽瑜, 张仲凯^*

云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业部西南作物基因资源与种质创制重点实验室, 昆明 650223

收稿日期:2014-09-04 修回日期:2014-10-10 出版日期:2014-11-01 发布日期:2014-11-21
通讯作者: 张仲凯
基金资助:
云南省创新团队;973前期专项

Cytopathological Characterization of Tobacco on Co-infection with Tomato zonate spot virus and Potato virus Y

Qi Fang^†, Jiahong Dong^†, Kuanyu Zheng, Zhongkai Zhang^*

Ministry of Agriculture, Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223

Received:2014-09-04 Revised:2014-10-10 Online:2014-11-01 Published:2014-11-21
Contact: Zhongkai Zhang

摘要/Abstract

摘要： 在自然情况下, 番茄环纹斑点病毒(TZSV)和马铃薯Y病毒(PVY)通常复合侵染同一植株。该文首次报道了TZSV和PVY复合侵染烟草(Nicotiana sp.)植株的细胞病理特征, 并与单独侵染进行了比较分析。复合侵染的烟草植株细胞中, TZSV病毒颗粒明显增多, 并聚集于囊膜内形成包涵体, 与PVY的风轮状及片层状内含体和病毒颗粒聚集体交叉分布于细胞质(内含线粒体明显增多)中, 线粒体、叶绿体和细胞核结构较完整; 两种病毒的颗粒、包涵体和内含体数量均较单一侵染增多, 且对寄主亚细胞结构的破坏较单一侵染为轻, TZSV和PVY及其与寄主的互作可能存在协生作用。

关键词: 番茄环纹斑点病毒, 马铃薯Y病毒, 烟草, 复合侵染, 细胞病理特征

Abstract: Tomato zonate spot virus (TZSV) and Potato virus Y (PVY) commonly co-infect the same plant under natural conditions. We compared the pathological characterization resulting from mixed and single infection of tobacco. Cells with mixed infection showed increased number of TZSV particles, and the particles clustered into the vesicles to form inclusion bodies, which cross-distributed in the cytoplasm with pinwheel and laminated inclusions and particles. The number of mitochondria was increased. The structure of mitochondria, chloroplasts, and the nucleus maintained relative integrity. The number of particles, inclusion bodies, and inclusions was increased but did not cause more severe damage on the subcellular structure when TZSV or PVY co-infected the same tobacco plant. A synergism may exist among TZSV, PVY and the plant host during co-infection.

Key words: Tomato zonate spot virus, Potato virus Y, tobacco, co-infection, cytopathological characterization

方琦, 董家红, 郑宽瑜, 张仲凯. 番茄环纹斑点病毒与马铃薯Y病毒复合侵染烟草的细胞病理特征. 植物学报, 2014, 49(6): 704-709.

Qi Fang, Jiahong Dong, Kuanyu Zheng, Zhongkai Zhang. Cytopathological Characterization of Tobacco on Co-infection with Tomato zonate spot virus and Potato virus Y. Chinese Bulletin of Botany, 2014, 49(6): 704-709.

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番茄环纹斑点病毒与马铃薯Y病毒复合侵染烟草的细胞病理特征

Cytopathological Characterization of Tobacco on Co-infection with Tomato zonate spot virus and Potato virus Y

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