不同时间的UV-B辐射对拟南芥幼苗生长的影响

doi:10.3724/SP.J.1259.2015.00040

植物学报 ›› 2015, Vol. 50 ›› Issue (1): 40-46.DOI: 10.3724/SP.J.1259.2015.00040

不同时间的UV-B辐射对拟南芥幼苗生长的影响

韩雯, 韩榕()

山西师范大学生命科学学院, 临汾 041004

收稿日期:2013-11-13 接受日期:2014-03-12 出版日期:2015-01-01 发布日期:2015-04-09
作者简介:
? 共同第一作者
基金资助:
国家自然科学基金(No.30671061)和山西省自然科学基金(No.20081101)

Effect of Different Times of UV-B Radiation on Seedling Growth of Arabidopsis thaliana

Wen Han, Rong Han^*

College of Life Sciences, Shanxi Normal University, Linfen 041004, China

Received:2013-11-13 Accepted:2014-03-12 Online:2015-01-01 Published:2015-04-09
Contact: Han Rong
About author:
? These authors contributed equally to this paper

摘要/Abstract

摘要： 以哥伦比亚野生型拟南芥(Arabidopsis thaliana)为实验材料, 用辐射功率为16.67 μW·cm^-2但不同时间(0.5、1、1.5、2、2.5和3小时)的UV-B辐射对拟南芥幼苗进行处理, 观察叶片形态, 并测定其根长、叶绿素含量、可溶性蛋白含量、丙二醛(MDA)浓度、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性及叶绿素荧光参数。结果显示, 短时间UV-B辐射可促进拟南芥根的伸长, 叶绿素和可溶性蛋白含量升高; 长时间UV-B辐射则抑制拟南芥根的生长, 使叶绿素含量、可溶性蛋白含量、叶绿素荧光参数F_v/F_m及qP逐渐降低, MDA浓度、SOD活性、CAT活性和qN值升高, 并随着时间的延长逐渐降低或升高。当辐射功率为16.67 μW·cm^-2时, 其最佳辐射时间为1.5小时。UV-B辐射作为一种环境胁迫, 其胁迫程度都是在一定的范围内, 当胁迫达到极限时, 植株都会对UV-B辐射产生一定的适应效应而使损伤降低。

Abstract: We treated Columbia-0 wild-type Arabidopsis thaliana seedlings with enhanced UV-B radiation (16.67 μW·cm^-2 radiant power) at 0.5, 1, 1.5, 2, 2.5, and 3 h. We observed leaf morphological characters and detected root length, leaf chlorophyll content, soluble protein content, malondialdehyde (MDA) concentration, superoxide dismutase (SOD) activity, catalase (CAT) activity and chlorophyll fluorescence characteristics. With a short period of UV-B radiation, roots were elongated and chlorophyll content and soluble protein content were increased in leaf, but prolonged UV-B radiation inhibited the elongated root length; caused decreased leaf chlorophyll content, soluble protein content and chlorophyll fluorescence characteristics such as F_v/F_m and qP; promoted MDA concentration; and increased SOD and CAT activity and qN over time. With 16.67 μW·cm^-2 radiation, the optimal time of UV-B radiation was 1.5 h. If UV-B radiation acts as a kind of environmental stress, the extent of damage to the plant was restricted, and the plant would have to adapt to UV-B radiation to let the damage reach a minimum when the stress level reached the limitation.

韩雯, 韩榕. 不同时间的UV-B辐射对拟南芥幼苗生长的影响. 植物学报, 2015, 50(1): 40-46.

Wen Han, Rong Han. Effect of Different Times of UV-B Radiation on Seedling Growth of Arabidopsis thaliana. Chinese Bulletin of Botany, 2015, 50(1): 40-46.

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链接本文: https://www.chinbullbotany.com/CN/10.3724/SP.J.1259.2015.00040

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图/表 8

图1 不同时间的UV-B辐射对拟南芥叶片形态的影响 (A) 对照; (B)-(G) B1-B6处理组, 辐射时间分别为0.5、1、1.5、2、2.5和3小时

Figure 1 The leaf morphological character of Arabidopsis thaliana with different periods of time of UV-B radiation (A) Control (CK); (B)-(G) B1-B6 treatments, with enhanced UV-B radiation at 0.5, 1, 1.5, 2, 2.5, and 3 h, respectively

图2 不同时间的UV-B辐射对拟南芥根长的影响CK: 对照; B1-B6同图1

Figure 2 The root length of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1

图3 不同时间的UV-B辐射对拟南芥叶片叶绿素含量的影响 CK: 对照; B1-B6同图1

Figure 3 The leaf chlorophyll content of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1

Figure 4 不同时间的UV-B辐射对拟南芥可溶性蛋白含量的影响 CK: 对照; B1-B6同图1

The soluble protein content of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1

Figure 5 不同时间的UV-B辐射对拟南芥丙二醛浓度的影响 CK: 对照; B1-B6同图1

The malondialdehyde concentration of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1

图6 不同时间的UV-B辐射对拟南芥超氧化物歧化酶活性的影响 CK: 对照; B1-B6同图1

Figure 6 The superoxide dismutase activity of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1

图7 不同时间的UV-B辐射对拟南芥过氧化氢酶活性的影响 CK: 对照; B1-B6同图1

Figure 7 The catalase activity of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1

图8 不同时间的UV-B辐射对拟南芥叶片叶绿素荧光特性的影响 CK: 对照; B1-B6同图1

Figure 8 The chlorophyll fluorescence characteristics of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1

参考文献 21

1	陈慧泽, 翟菁如, 杜美婷, 韩榕 (2011). 增强UV-B辐射对小麦根尖分裂期细胞肌动蛋白的影响. 植物分类与资源学报 33, 306-310.
2	郭爱华, 高丽美, 李永锋, 翟菁茹, 韩榕 (2010). 增强紫外线B辐射对小麦根尖细胞微管骨架的影响. 中国细胞生物学学报 32, 256-260.
3	韩榕 (2002). He-Ne激光对小麦增强UV-B辐射损伤的修复效应及机理. 博士论文. 西安: 西北大学.
4	李合生 (2000). 植物生理生化实验原理和技术. 北京: 高等教育出版社. pp. 185-186.
5	李鹏民, 高辉远, Strasser RJ (2005). 快速叶绿素荧光诱导动力学分析在光合作用研究中的应用. 植物生理与分子生物学学报 31, 559-566.
6	李晓阳, 陈慧泽, 韩榕 (2013). UV-B辐射对拟南芥种子萌发和幼苗生长的影响. 植物学报 48, 52-58.
7	李元, 祖艳群, 高召华, 高光凯 (2006). UV-B辐射对报春花的生理生化效应. 西北植物学报 26, 179-182.
8	罗丽琼, 陈宗瑜, 周平, 温永琴, 丁金玲 (2008). 低纬高原地区UV-B辐射对报春花丙二醛、蛋白质含量的影响. 广西植物 28, 130-135.
9	马旭俊, 朱大海 (2003). 植物超氧化物歧化酶(SOD)的研究进展. 遗传 25, 225-231.
10	王学奎 (2006). 植物生理生化实验原理和技术(第2版). 北京: 高等教育出版社. pp. 169-170.
11	魏晓雪, 于景华, 李德文, 佟璐, 庞海河, 祖元刚 (2011). UV-B辐射增强对红松幼苗针叶脂质过氧化及抗氧化系统的影响. 林业科学 47(5), 54-59.
12	吴少伯 (1979). 植物组织中蛋白质及同功酶的聚丙烯酰胺凝胶盘状电泳. 植物生理学通讯 (1), 30-33.
13	晏斌, 戴秋杰 (1996). 紫外线B对水稻叶组织中活性氧代谢及膜系统的影响. 植物生理学报 22, 373-378.
14	颜其德, 康建成 (2005). 地球生命的保护伞——臭氧层. 科学 57(6), 54-56.
15	张红霞, 吴能表, 胡丽涛, 洪鸿 (2010). 不同强度UV-B辐射胁迫对蚕豆幼苗生长及叶绿素荧光特性的影响. 西南师范大学学报(自然科学版) 35, 105-110.
16	张美萍, 江玉珍, 于光辉, 苑中原, 陈国祥 (2009). 稀土元素对增强UV-B辐照下小麦抗氧化酶的影响. 核农学报 23, 316-319.
17	张永峰, 殷波 (2009). 混合盐碱胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响. 草业学报 18, 46-50.
18	张志良, 瞿伟菁, 李小方 (2009). 植物生理学实验指导(第4版). 北京: 高等教育出版社. pp. 58-220.
19	Lowry OH, Rosebrough NT, Farr AL, Randall RJ (1951). Protein measurement with the folin phenol reagent.J Biol Chem 193, 265-275.
20	Ranieri A, Lencioni L, Schenone G, Soldatini GF (1993). Glutathione-ascorbic acid cycle in pumpkin plants grown under polluted air in open-top chambers.J Plant Physiol 142, 286-290.
21	Uphem BL, Jahnke LS (1986). Photooxidative reactions in chloroplast thylakoids: evidence for a fenton-type reaction promoted by superoxide or ascorbate.Photosynth Res 8, 235-247.

不同时间的UV-B辐射对拟南芥幼苗生长的影响

Effect of Different Times of UV-B Radiation on Seedling Growth of Arabidopsis thaliana

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