水稻高代回交置换系穗颈长度的遗传分析

doi:10.3724/SP.J.1259.2015.00032

植物学报 ›› 2015, Vol. 50 ›› Issue (1): 32-39.DOI: 10.3724/SP.J.1259.2015.00032

水稻高代回交置换系穗颈长度的遗传分析

赵春芳, 强新涛, 张亚东, 朱镇, 陈涛, 赵庆勇, 周丽慧, 姚姝, 于新, 王才林^*()

江苏省农业科学院粮食作物研究所, 江苏省优质水稻工程技术研究中心, 国家水稻改良中心南京分中心, 南京 210014

收稿日期:2014-02-19 接受日期:2014-08-25 出版日期:2015-01-01 发布日期:2015-04-09
通讯作者: 王才林
作者简介:
? 共同第一作者
基金资助:
国家自然科学基金(No.31200144)和现代农业产业技术体系建设专项资金项目(No.CARS-01-47)

ygenase that epoxidizes gibberellins in a novel deactivation reaction in rice

Chunfang Zhao, Xintao Qiang, Yadong Zhang, Zhen Zhu, Tao Chen, Qingyong Zhao, Lihui Zhou, Shu Yao, Xin Yu, Cailin Wang^*

Nanjing Branch of Chinese National Center for Rice Improvement, Jiangsu High Quality Rice Research and Development Center, Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2014-02-19 Accepted:2014-08-25 Online:2015-01-01 Published:2015-04-09
Contact: Wang Cailin
About author:
? These authors contributed equally to this paper

摘要/Abstract

摘要： 对以籼稻品种9311为受体、粳稻品种日本晴为供体的3个控制穗颈长度性状的高代回交置换系(C031、C108和C115)进行了农艺性状测定和遗传基础分析。结果表明, 除穗颈长度外, 3个置换系的株高与9311之间也存在显著或极显著差异, 置换系C031的每穗总粒数和千粒重与9311差异显著。通过遗传背景检测, 发现置换系C031和C115均含有2个日本晴置换片段, 置换系C108含有3个日本晴置换片段。遗传分析表明, 3个F₂分离群体中穗颈长度的分离均由单个孟德尔因子控制, 其加性效应分别为3.09、3.05和-2.04。连锁分析表明, C031和C108与携带置换片段上的分子标记均不连锁, C115与第12号染色体的分子标记存在不同程度的连锁, 表明控制C115穗颈长度表型的基因位于第12号染色体上, 将其命名为qPNL- 12。研究结果为精细定位和克隆该基因奠定了基础。

Abstract: We analyzed the main agronomy traits and genetic basis of three advanced backcross substitution lines (C031, C108 and C115) derived from an indica recipient, 9311 and a japonica donor, Nipponbare. Compared with 9311, the three substitution lines showed significant differences in panicle neck length and plant height. In addition, C031 and 9311 differed in grain number per panicle and grain weight. Genetic background detection showed that C031 and C115 contain two substituted segments from Nippobare, while C108 contains three segments. Genetic analysis indicated that the ratios of panicle neck length in three F₂ segregating populations were all controlled by a single Mendelian factor, and their additive effects were 3.09, 3.05 and -2.04, respectively. Linkage analysis showed that the molecular markers from substituted segments of C031 and C108 were not linked with their segregating populations. Markers on chromosome 12 from substituted segments of C115 linked well with its segregating population, so the gene controlling panicle neck length in C115 is located on chromosome 12 and named qPNL-12. These results provide useful information for mapping and cloning new rice genes controlling panicle neck elongation.

赵春芳, 强新涛, 张亚东, 朱镇, 陈涛, 赵庆勇, 周丽慧, 姚姝, 于新, 王才林. 水稻高代回交置换系穗颈长度的遗传分析. 植物学报, 2015, 50(1): 32-39.

Chunfang Zhao, Xintao Qiang, Yadong Zhang, Zhen Zhu, Tao Chen, Qingyong Zhao, Lihui Zhou, Shu Yao, Xin Yu, Cailin Wang. ygenase that epoxidizes gibberellins in a novel deactivation reaction in rice. Chinese Bulletin of Botany, 2015, 50(1): 32-39.

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图/表 6

Figure 1 亲本9311及3个高代回交置换系的穗颈表型箭头表示穗颈节位置, 短线表示剑叶叶枕位置

Panicle phenotypes of recipient 9311 and 3 advanced backcross substitution lines Arrows indicate the base of panicle, and short lines indicate the base of flag leaf

图2 3个置换系与亲本9311的主要农艺性状差异 *和**分别表示该置换系与9311间差异达显著(P<0.05)和极显著(P<0.01)水平(t检验)

Figure 2 Differences of main agronomic traits between three substitution lines and recipient 9311 * and ** represent the significant differences between the substitution lines and 9311 at 0.05 and 0.01 level, respectively (by LSD-t test)

图3 3个置换系的置换片段分析 (A) 置换片段在水稻12条染色体上的分布, 方框表示日本晴置换片段, 双线表示亲本9311的基因组; (B) 置换片段在水稻高密度物理图谱上的位置和长度, 染色体左侧数字代表分子标记的物理距离(Mb), 右侧为标记名称

Figure 3 Analysis of substituted segments of three substitution lines (A) Distribution of substituted segments on 12 rice chromosomes; Boxes refer to substituted segments of Nipponbare; double lines show the genome of recipient 9311; (B) Location and length of these substituted segments on high-density physical map in rice; The physical location (Mb) and names of molecular markers were indicated in the left and right side of the chromosome, respectively

表1 3个F2分离群体穗颈长度的分离比例

Table 1 Segregation ratio of panicle neck length in three F2 populations

Combination	No. of plants	No. of plants with long panicle neck	No. of plants with intermediate type	No. of plants with short panicle neck	Expect ratio	χ²
9311/C031 9311/C108 9311/C115	78 80 79	60		18	3:1 1:2:1 3:1	0.15 3.48 0.21
		27	37	16
		61		18

图4 C031 (A)、C108 (B)、C115 (C)与9311的F2群体穗颈长度分布黑框表示用于检测与穗颈长度有连锁关系的靶区间的单株

Figure 4 Distribution of the panicle neck length in three F2 segregating populations between C031 (A), C108 (B), C115 (C) and 9311 Black box represents the individuals used for detecting the target substitution segments controlling the panicle neck length trait

表2 3个置换系穗颈长度的遗传效应分析

Table 2 Genetic effect of panicle neck length for three substitution lines

Substitution line	Panicle neck length for higher parent	Panicle neck length for lower parent	Panicle neck length for F₁ (cm)	Mean of the parents (cm)	Additive effect (a) (cm)	Dominant effect (d) (cm)	Degree of dominance (d·a^-1)
C031 C108 C115	11.48 11.40 5.30	5.30 5.30 1.22	9.17 7.38 4.50	8.39 8.35 3.26	3.09 3.05 2.04	0.78 -0.97 1.24	0.25 -0.32 0.61

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水稻高代回交置换系穗颈长度的遗传分析

ygenase that epoxidizes gibberellins in a novel deactivation reaction in rice

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