植物学报 ›› 2019, Vol. 54 ›› Issue (1): 37-45.DOI: 10.11983/CBB18019

• 研究报告 • 上一篇    下一篇

利用荧光标记SSR鉴别21个茶花新品种

陶乃奇1,张斌2,3,刘信凯4,周和达2,钟乃盛4,严丹峰4,张敏1,高继银4,张文驹1,*()   

  1. 1 复旦大学生命科学学院生物多样性科学研究所, 生物多样性与生态工程教育部重点实验室, 上海 200433
    2 上海星源农业实验场, 上海 201417
    3 上海市农业发展促进中心, 上海 200335
    4 棕榈生态城镇发展股份有限公司, 广州 510627
  • 收稿日期:2018-01-18 接受日期:2018-04-28 出版日期:2019-01-30 发布日期:2019-07-31
  • 通讯作者: 张文驹
  • 基金资助:
    国家自然科学基金(31670223);上海市农委农业种质资源创新与良种良法配套技术集成应用沪农科种字(2015第10号No.2016F2FC0012)

Identification of 21 New Camellia Hybrid Varieties by Fluorescence-labelled Simple Sequence Repeat Markers

Naiqi Tao1,Bin Zhang2,3,Xinkai Liu4,Heda Zhou2,Naisheng Zhong4,Danfeng Yan4,Min Zhang1,Jiyin Gao4,Wenju Zhang1,*()   

  1. 1 Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, School of Life Sciences, Fudan University, Shanghai 200433, China
    2 Shanghai Xingyuan Agricultural Experiment Center, Shanghai 201417, China
    3 Shanghai Agricultural Development and Promotion Center, Shanghai 200335, China
    4 Palm Eco-Town Development Co, Ltd, Guangzhou 510627, China
  • Received:2018-01-18 Accepted:2018-04-28 Online:2019-01-30 Published:2019-07-31
  • Contact: Wenju Zhang

摘要: 利用杜鹃红山茶(Camellia azalea)转录组数据和前人研究中获得的12个高多态性的SSR位点, 采用荧光毛细管电泳分析了21个茶花杂交新品种的基因型。结果显示, 12对SSR引物都能获得稳定清晰的扩增产物, 且上述12个SSR位点的基因型能够完全区分上述21个茶花新品种。其中, 来自同一杂交组合后代的多个品种, 基因型间有差异的位点数为2-10个, 来自不同杂交组合后代的品种间, 其基因型有差异的位点数为5-12个。研究结果表明, 上述21个茶花新品种均获得了独特的基因型标记, 能准确地进行品种身份鉴定, 这对以扦插或嫁接扩大生产的茶花品种的鉴定和品种权保护具有重要意义。

关键词: 山茶属, 荧光标记SSR, 新品种保护, 品种鉴定

Abstract: In this study, 12 highly polymorphic simple sequence repeat (SSR) loci were selected from Camellia azalea transcriptome data and previous studies, and the genotypes of 21 new Camellia hybrid varieties were analyzed by fluorescence capillary electrophoresis. Repeatable and distinct amplification products could be obtained at 12 SSR loci from all samples, and 21 new varieties could be identified by the specific genotype of 12 SSR loci. Significantly, for the 21 new varieties, the number of discrepant loci was 2 to 10 among varieties from the same parental combination but 5 to 12 among varieties from different combinations. Hence, each of the 21 varieties was marked by a unique group of molecular markers, which can be used to identify these varieties accurately. These markers are very useful for identifying and protecting Camellia varieties, especially for those propagated that are produced by grafting or cutting.

Key words: Camellia, fluorescence labelled SSR, new variety protection, variety identification