植物学报 ›› 2019, Vol. 54 ›› Issue (1): 58-63.DOI: 10.11983/CBB17237

• 研究报告 • 上一篇    下一篇

茄子种质遗传多样性及群体结构的SRAP分析

谢立峰1,李宁2,*(),李烨1,*(),姚明华2   

  1. 1 哈尔滨市农业科学院, 哈尔滨 150028
    2 湖北省农业科学院经济作物研究所, 武汉 430064
  • 收稿日期:2017-12-06 接受日期:2018-08-21 出版日期:2019-01-30 发布日期:2019-07-31
  • 通讯作者: 李宁,李烨
  • 基金资助:
    国家重点研发计划(2016YFD0101703);哈尔滨市应用技术研究与开发项目(2016RQYY0020);黑龙江省应用技术研究与开发计划(CA15B103-1)

Genetic Diversity and Population Structure of Eggplant (Solanum melongena) Germplasm Resources Based on SRAP Method

Lifeng Xie1,Ning Li2,*(),Ye Li1,*(),Minghua Yao2   

  1. 1 Harbin Academy of Agricultural Sciences, Harbin 150028, China
    2 Institute of Economic Crops of Hubei Academy of Agricultural Sciences, Wuhan 430064, China
  • Received:2017-12-06 Accepted:2018-08-21 Online:2019-01-30 Published:2019-07-31
  • Contact: Ning Li,Ye Li

摘要: 采用SRAP标记法对183份茄子(Solanum melongena)种质资源的遗传关系和群体结构进行分析。结果表明, 33对多态性SRAP引物组合共扩增出215条清晰稳定的多态性条带, 平均每对引物组合产生7条多态性条带。183份茄子种质的遗传相似系数介于0.276-0.813之间, 平均值为0.623, 表明茄子种质资源间遗传背景存在一定的差异。在遗传距离为0.345 6处可将183份茄子种质分为4组。通过群体结构分析可将种质划分为4个群体, 不同群体间的界限十分明显, 且群体间的基因渗透较高。

关键词: 茄子, 遗传多样性, 群体结构, SRAP分析

Abstract: The genetic diversity and the population structure of 183 germplasm resources of eggplant were analyzed by using SRAP markers. Overall, 215 bands were generated with 7 binds per primer on average. The mean genetic similarity coefficient was 0.623 among the cultivars (0.276-0.813), which suggested genetic differentiation among the germplasm resources. The germplasm resources were divided into four groups with a similarity coefficient of 0.345 6. Introgression exists in the four groups based on population structure analysis. The boundaries among different groups are obvious, and gene introgression among varieties is high.

Key words: eggplant, genetic diversity, population structure, SRAP analysis