Abstract: Esterase(EST)isozymes in clove of 18 cultivars from 3 ecotypes of Chinese Allium sativum L. were studied using vertical slab polyacrylamide gel electrophoresis (PAGE). And the phylogenetic relationship of these cultivars were estimated by ordination analysis. The results indicated that 18 garlic varieties could be divided into three varietases, 1.Russia varietas groups; 2.Jimusaer varietas groups; 3.China inland varietas groups. The last group could be divided into five variety groups: ①Guanzhong variety group; ②Northwestern variety group; ③Southwestern variety group; ④Yun_gui variety group; ⑤East China variety group. The ecotypes of garlic is not equivalent to the genetypes. Phylogenetic relationship of garlic may be reflected by the changes of esterase isozyme.