植物学报 ›› 2001, Vol. 18 ›› Issue (03): 371-374.

• 研究论文 • 上一篇    下一篇

四倍体大燕麦与六倍体裸燕麦的受精作用和胚胎发育

赵云云 刘家熙 杨才   

  1. 1(首都师范大学生物系 北京 100037)2(河北省张家口坝下农科所 张家口 075131)
  • 收稿日期:2000-04-21 修回日期:2000-06-08 出版日期:2001-05-20 发布日期:2001-05-20
  • 通讯作者: 赵云云

Fertilization and Embryo Development in Hybridization between Avena magna and Avena nuda

ZHAO Yun-Yun LIU Jia-Xi YANG Cai   

  1. (Department of Biology, Capital Normal University, Beijing 100037)(Baxia Institute of Agricultural Science, Zhangjiakuo 075131)
  • Received:2000-04-21 Revised:2000-06-08 Online:2001-05-20 Published:2001-05-20

摘要: 用石蜡切片法,对四倍体大燕麦(Avena magna L.)和六倍体裸燕麦(Avena nuda L.)杂交的受精作用和胚胎发育进行了观察。结果表明,六倍体裸燕麦花粉在四倍体大燕麦柱头上萌发良好,花粉管可顺利长入花柱和胚囊。观察的168个四倍体大燕麦子房中,238%发生了双受精,产生了胚和胚乳;179%发生了单卵受精,只产生胚乳而无胚;总受精率为536%;成胚率为417%。由于胚乳的缺乏或发育异常及败育,最终难以获得有生活力的种子。为四倍体大燕麦和六倍体裸燕麦杂交提供了细胞胚胎学证据。

Abstract: Observations were made on the fertilization and early embryo development in intergeneric cross between Avena magna and Avena nuda. The pollen germination of Avena nada appeared to be normal on the stigma of Avena magna and the pollen tubes grew into the style and entered the embryo sacs. In 168 florets fixed after pollination, 2.38% had double fertilization and produced embryo and endosperm, but the endosperm development was slower than that of the embryo, 1.79% had only embryo and 1.19% had only endosperm. The frequency of fertilization and embryo formation were 5.36% and 4.17% respectively. Due to the lack of endosperm or its abnormal development and abortion, no viable seeds were poduced. Cytoembryological evidence was provided for crosses between Avena magna and Avena nuda.