植物学报 ›› 2005, Vol. 22 ›› Issue (01): 54-57.

• 组织培养简讯 • 上一篇    下一篇

灯盏细辛的组织培养与快速繁殖

吴毅歆 谢庆华 桑林 谢世清   

  1. 1(云南省农科院生物技术研究所 昆明 650223) 2(云南师范大学生物资源技术研究所 昆明 650031)
  • 收稿日期:2003-09-01 修回日期:2003-12-22 出版日期:2005-02-25 发布日期:2005-02-25
  • 通讯作者: 谢庆华

Tissue Culture and Micropropagation of Erigeron breviscapus

WU Yi-Xin XIE Qing-Hua SANG Lin XIE Shi-Qing   

  1. 1(Institute of Biotechnology,Yunnan Academy of Agricultural Science, Kunming 650223) 2(Technological Research Institute of Biological Resource,Yunnan Normal University, Kunming 650031)
  • Received:2003-09-01 Revised:2003-12-22 Online:2005-02-25 Published:2005-02-25
  • Contact: XIE Qing-Hua

摘要: 本研究以野生高含量灯盏花的叶片为外植体,在7个不同浓度NAA和BA组合的MS培养基上进行愈伤组织诱导、不定芽分化和增殖及生根的培养,确定了灯盏花快繁体系的最适培养条件:(1)初代培养基:(MS+6-BA) 0.5 mg.L-1+NAA0.1 mg.L-1;(2)丛生芽增殖培养基:(MS+6-BA) 2 mg.L-1+NAA 0.7 mg.L-1;(3) 生根培养基:(2/3MS+NAA) 0.5 mg.L-1+IBA0.8。

Abstract: The leaves of Erigeron breviscapus were used as explants to induce calli, buds and rooting in seven culture media. The optimal media were (1) initial medium, MS+6-BA (1.5 mg.L-+NAA (0.3 mg.L-1); (2) clump bud generation medium, MS+6-BA (1 mg.L-1); and (3) rooting medium,2/3 MS+NAA (0.3 mg.L-1).