病毒介导的植物基因组编辑技术研究进展

doi:10.11983/CBB23046

植物学报 ›› 2024, Vol. 59 ›› Issue (3): 452-462.DOI: 10.11983/CBB23046 cstr: 32102.14.CBB23046

病毒介导的植物基因组编辑技术研究进展

胡丹玲, 孙永伟^*()

内蒙古大学生命科学学院, 呼和浩特 010000

收稿日期:2023-04-07 接受日期:2023-08-07 出版日期:2024-05-10 发布日期:2024-05-10
通讯作者: *孙永伟, 内蒙古大学生命科学学院副教授、硕士生导师。入选内蒙古自治区高等学校青年科技英才计划。先后主持国家自然科学基金项目3项, 内蒙古自然科学基金面上项目3项。主要从事牧草生物学及牧草基因组编辑体系建立相关研究。在Molecular Plant、Plant Biotechnology Journal和Frontiers in Plants Science等期刊发表学术论文10余篇, 授权国家发明专利10余项。E-mail: sunyongwei@imu.edu.cn
基金资助:
国家自然科学基金青年科学基金(31800206);国家自然科学基金地区科学基金(32160111);内蒙古自治区自然科学基金面上项目(2020MS03027)

Advances in Virus-mediated Genome Editing Technology in Plants

Danling Hu, Yongwei Sun^*()

School of Life Sciences, Inner Mongolia University, Hohhot 010000, China

Received:2023-04-07 Accepted:2023-08-07 Online:2024-05-10 Published:2024-05-10
Contact: *E-mail: sunyongwei@imu.edu.cn

摘要/Abstract

摘要： CRISPR/Cas作为一种新兴的靶向基因组编辑技术, 具有操作过程简便、编辑效率高和支持多靶点编辑等优势, 在植物遗传育种中应用前景广阔。然而对于一些尚未建立遗传转化体系和再生体系的植物, 基因组编辑技术的应用仍然受限。病毒介导的植物基因组编辑技术可不依赖遗传转化和再生等步骤, 即可快速获得无外源转基因成分的基因组编辑植物, 受到广泛关注。该文主要介绍了病毒介导的CRISPR/Cas植物基因组编辑技术的工作原理及优势, 系统总结了该技术在植物基因组编辑领域的应用现状, 并重点讨论了该技术体系存在的问题及挑战, 以期为深入开展这一领域研究提供参考。

关键词: 病毒, 植物, 基因组编辑, CRISPR/Cas

Abstract: As a new technology for targeted genome editing, clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (Cas) have the advantages of easy operation, high editing efficiency, and support for multi-target editing, thus showing wide application prospects in plant genetic breeding. However, the process in plants relies mainly on Agrobacterium- or particle bombardment-mediated genetic transformation, which is time-consuming as well as species- and varieties-dependent. Virus-mediated plant genome editing has attracted extensive atten- tion because of its no requirement of genetic transformation and plant regeneration. In this review, we introduce the working principle and advantages of virus-mediated CRISPR/Cas plant genome editing technology, systematically sum- marize the current application status of this technology in the field of plant genome editing, and focus on discussing the problems and challenges of this technology system, aiming to provide reference for further research and development in this field.

Key words: virus, plant, genome editing, CRISPR/Cas

胡丹玲, 孙永伟. 病毒介导的植物基因组编辑技术研究进展. 植物学报, 2024, 59(3): 452-462.

Danling Hu, Yongwei Sun. Advances in Virus-mediated Genome Editing Technology in Plants. Chinese Bulletin of Botany, 2024, 59(3): 452-462.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB23046

https://www.chinbullbotany.com/CN/Y2024/V59/I3/452

图/表 2

参考文献 65

[1]	Ali Z, Abul-Faraj A, Li LX, Ghosh N, Piatek M, Mahjoub A, Aouida M, Piatek A, Baltes NJ, Voytas DF, Dinesh- Kumar S, Mahfouz MM (2015a). Efficient virus-mediated genome editing in plants using the CRISPR/Cas9 system. Mol Plant 8, 1288-1291.
[2]	Ali Z, Abul-Faraj A, Piatek M, Mahfouz MM (2015b). Activity and specificity of TRV-mediated gene editing in plants. Plant Signal Behav 10, e1044191.
[3]	Ali Z, Eid A, Ali S, Mahfouz MM (2018). Pea early-browning virus-mediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis. Virus Res 244, 333-337.
[4]	Al-Shayeb B, Skopintsev P, Soczek KM, Stahl EC, Li Z, Groover E, Smock D, Eggers AR, Pausch P, Cress BF, Huang CJ, Staskawicz B, Savage DF, Jacobsen SE, Banfield JF, Doudna JA (2022). Diverse virus-encoded CRISPR-Cas systems include streamlined genome editors. Cell 185, 4574-4586. DOI PMID
[5]	Ariga H, Toki S, Ishibashi K (2020). Potato virus X vector- mediated DNA-free genome editing in plants. Plant Cell Physiol 61, 1946-1953.
[6]	Avesani L, Marconi G, Morandini F, Albertini E, Bruschet-ta M, Bortesi L, Pezzotti M, Porceddu A (2007). Stability of Potato virus X expression vectors is related to insert size: implications for replication models and risk assessment. Transgenic Res 16, 587-597. PMID
[7]	Barrangou R (2014). Cas9 targeting and the CRISPR revolution. Science 344, 707-708. DOI PMID
[8]	Bernabé-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, Garcia-Carpintero V, Gianoglio S, Vázquez-Vilar M, Granell A, Orzaez D (2019). Assessment of Cas12a-mediated gene editing efficiency in plants. Plant Biotechnol J 17, 1971-1984. DOI PMID
[9]	Bigelyte G, Young JK, Karvelis T, Budre K, Zedaveinyte R, Djukanovic V, Van Ginkel E, Paulraj S, Gasior S, Jones S, Feigenbutz L, Clair GS, Barone P, Bohn J, Acharya A, Zastrow-Hayes G, Henkel-Heinecke S, Silanskas A, Seidel R, Siksnys V (2021). Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modifi-cation in cells. Nat Commun 12, 6191. DOI PMID
[10]	Cai QN, Guo DM, Cao YJ, Li Y, Ma R, Liu WP (2022). App- lication of CRISPR/CasΦ2 system for genome editing in plants. Int J Mol Sci 23, 5755.
[11]	Cao XS, Xie HT, Song ML, Lu JH, Ma P, Huang BY, Wang MG, Tian YF, Chen F, Peng J, Lang ZB, Li GF, Zhu JK (2022). Cut-dip-budding delivery system enables genetic modifications in plants without tissue culture. Innovation (Camb) 4, 100345.
[12]	Chapman S, Faulkner C, Kaiserli E, Garcia-Mata C, Savenkov EI, Roberts AG, Oparka KJ, Christie JM (2008). The photoreversible fluorescent protein iLOV outperforms GFP as a reporter of plant virus infection. Proc Natl Acad Sci USA 105, 20038-20043. DOI PMID
[13]	Chen H, Su ZQ, Tian B, Liu Y, Pang YH, Kavetskyi V, Trick HN, Bai GH (2022). Development and optimization of a Barley stripe mosaic virus-mediated gene editing system to improve Fusarium head blight resistance in wheat. Plant Biotechnol J 20, 1018-1020.
[14]	Chen KL, Wang YP, Zhang R, Zhang HW, Gao CX (2019). CRISPR/Cas genome editing and precision plant breeding in agriculture. Annu Rev Plant Biol 70, 667-697. DOI PMID
[15]	Chiong KT, Cody WB, Scholthof HB (2021). RNA silencing suppressor-influenced performance of a virus vector de- livering both guide RNA and Cas9 for CRISPR gene editing. Sci Rep 11, 6769.
[16]	Cody WB, Scholthof HB, Mirkov TE (2017). Multiplexed gene editing and protein overexpression using a Tobacco mosaic virus viral vector. Plant Physiol 175, 23-35.
[17]	Creager ANH, Scholthof KBG, Citovsky V, Scholthof HB (1999). Tobacco mosaic virus: pioneering research for a century. Plant Cell 11, 301-308. PMID
[18]	Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas DF (2020). Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs. Nat Plants 6, 620-624. DOI PMID
[19]	Ganesan U, Bragg JN, Deng M, Marr S, Lee MY, Qian SS, Shi ML, Kappel J, Peters C, Lee Y, Goodin MM, Dietzgen RG, Li ZH, Jackson AO (2013). Construction of a Sonchus yellow net virus minireplicon: a step toward re- verse genetic analysis of plant negative-strand RNA viru- ses. J Virol 87, 10598-10611. DOI PMID
[20]	Gao CX (2018). The future of CRISPR technologies in agriculture. Nat Rev Mol Cell Biol 19, 275-276.
[21]	Gao Q, Xu WY, Yan T, Fang XD, Cao Q, Zhang ZJ, Ding ZH, Wang Y, Wang XB (2019). Rescue of a plant cytorhabdo virus as versatile expression platforms for planthopper and cereal genomic studies. New Phytol 223, 2120-2133.
[22]	Ghoshal B, Vong B, Picard CL, Feng SH, Tam JM, Jacobsen SE (2020). A viral guide RNA delivery system for CRISPR-based transcriptional activation and heritable targeted DNA demethylation in Arabidopsis thaliana. PLo- S Genet 16, e1008983.
[23]	He XL, Liu PC, Ma BJ, Chen XF (2022). Advance in gene- editing technology based on CRISPR/Cas9 and its appli- cation in plants. Chin Bull Bot 57, 508-531. (in Chinese)
	何晓玲, 刘鹏程, 马伯军, 陈析丰 (2022). 基于CRISPR/Cas9的基因编辑技术研究进展及其在植物中的应用. 植物学报 57, 508-531. DOI
[24]	He YB, Zhang T, Sun H, Zhan HD, Zhao YD (2020). A reporter for noninvasively monitoring gene expression and plant transformation. Hortic Res 7, 152.
[25]	Hu JC, Li SY, Li ZL, Li HY, Song WB, Zhao HM, Lai JS, Xia LQ, Li DW, Zhang YL (2019). A barley stripe mosaic virus-based guide RNA delivery system for targeted mutagenesis in wheat and maize. Mol Plant Pathol 20, 1463-1474. DOI PMID
[26]	Jackson AO, Dietzgen RG, Goodin MM, Bragg JN, Deng M (2005). Biology of plant rhabdoviruses. Annu Rev Phytopathol 43, 623-660. PMID
[27]	Jiang N, Zhang C, Liu JY, Guo ZH, Zhang ZY, Han CG, Wang Y (2019). Development of Beet necrotic yellow vein virus-based vectors for multiple-gene expression and guide RNA delivery in plant genome editing. Plant Biotechnol J 17, 1302-1315. DOI PMID
[28]	Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E (2012). A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816-821. DOI PMID
[29]	Kaya H, Ishibashi K, Toki S (2017). A split Staphylococcus aureus Cas9 as a compact genome-editing tool in plants. Plant Cell Physiol 58, 643-649.
[30]	Kim DY, Lee JM, Moon SB, Chin HJ, Park S, Lim Y, Kim D, Koo T, Ko JH, Kim YS (2022). Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Nat Biotechnol 40, 94-102.
[31]	Kim H, Oh Y, Park E, Kang M, Choi Y, Kim SG (2023). Heritable virus-induced genome editing (VIGE) in Nicotiana attenuata. In: Nicotiana attenuata. In: Bae S, Song B, eds. Base Editors: Methods and Protocols. New York: Humana. pp. 203-218.
[32]	Lee WS, Hammond-Kosack KE, Kanyuka K (2012). Barley stripe mosaic virus-mediated tools for investigating gene function in cereal plants and their pathogens: virus-induced gene silencing, host-mediated gene silencing, and virus- mediated overexpression of heterologous protein. Plant Physiol 160, 582-590.
[33]	Lei JF, Dai PH, Li Y, Zhang WQ, Zhou GT, Liu C, Liu XD (2021). Heritable gene editing using FT mobile guide RNAs and DNA viruses. Plant Methods 17, 20.
[34]	Lei JF, Li Y, Dai PH, Liu C, Zhao Y, You YZ, Qu YY, Chen QJ, Liu XD (2022). Efficient virus-mediated genome editing in cotton using the CRISPR/Cas9 system. Front Plant Sci 13, 1032799.
[35]	Li SY, Li JY, He YB, Xu ML, Zhang JH, Du WM, Zhao YD, Xia LQ (2019). Precise gene replacement in rice by RNA transcript-templated homologous recombination. Nat Biotechnol 37, 445-450. DOI PMID
[36]	Li SY, Xia LQ (2020). Precise gene replacement in plants through CRISPR/Cas genome editing technology: current status and future perspectives. aBIOTECH 1, 58-73. DOI PMID
[37]	Li TD, Hu JC, Sun Y, Li BS, Zhang DL, Li WL, Liu JX, Li DW, Gao CX, Zhang YL, Wang YP (2021). Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture. Mol Plant 14, 1787-1798. DOI PMID
[38]	Li Z, Zhong ZH, Wu ZS, Pausch P, Al-Shayeb B, Amerasekera J, Doudna JA, Jacobsen SE (2023). Genome editing in plants using the compact editor casΦ. Proc Natl Acad Sci USA 120, e2216822120.
[39]	Liang Z, Chen KL, Li TD, Zhang Y, Wang YP, Zhao Q, Liu JX, Zhang HW, Liu CM, Ran YD, Gao CX (2017). Effi- cient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes. Nat Commun 8, 14261. DOI PMID
[40]	Lin CS, Hsu CT, Yang LH, Lee LY, Fu JY, Cheng QW, Wu FH, Hsiao HCW, Zhang YS, Zhang R, Chang WJ, Yu CT, Wang W, Liao LJ, Gelvin SB, Shih MC (2018). Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration. Plant Biotechnol J 16, 1295-1310.
[41]	Liu HW, Zhang BH (2020). Virus-based CRISPR/Cas9 genome editing in plants. Trends Genet 36, 810-813. DOI PMID
[42]	Liu Q, Zhao CL, Sun K, Deng YL, Li ZH (2023). Engineered biocontainable RNA virus vectors for non-transgenic ge- nome editing across crop species and genotypes. Mol Plant 16, 616-631.
[43]	Liu SS, Sretenovic S, Fan TT, Cheng YH, Li G, Qi A, Tang X, Xu Y, Guo WJ, Zhong ZH, He Y, Liang YL, Han QQ, Zheng XL, Gu XF, Qi YP, Zhang Y (2022). Hypercompact CRISPR-Cas12j2 (CasΦ) enables genome editing, gene activation, and epigenome editing in plants. Plant Commun 3, 100453.
[44]	Liu YG, Li GS, Zhang YL, Chen LT (2019). Current advances on CRISPR/Cas genome editing technologies in plants. J South China Agricul Univ 40(5), 38-49. (in Chinese)
	刘耀光, 李构思, 张雅玲, 陈乐天 (2019). CRISPR/Cas植物基因组编辑技术研究进展. 华南农业大学学报 40(5), 38-49.
[45]	Luo YJ, Na R, Nowak JS, Qiu Y, Lu QS, Yang CY, Marsolais F, Tian LN (2021). Development of a Csy4-processed guide RNA delivery system with soybean-infecting virus ALSV for genome editing. BMC Plant Biol 21, 419. DOI PMID
[46]	Ma XN, Zhang XY, Liu HM, Li ZH (2020). Highly efficient DNA-free plant genome editing using virally delivered CRISPR-Cas9. Nat Plants 6, 773-779. DOI PMID
[47]	Mei Y, Beernink BM, Ellison EE, Konečná E, Neelakandan AK, Voytas DF, Whitham SA (2019). Protein expression and gene editing in monocots using foxtail mosaic virus vectors. Plant Direct 3, e00181.
[48]	Nagalakshmi U, Meier N, Liu JY, Voytas DF, Dinesh-Kumar SP (2022). High-efficiency multiplex biallelic heritable editing in Arabidopsis using an RNA virus. Plant Physiol 189, 1241-1245. DOI PMID
[49]	Oh Y, Kim H, Lee HJ, Kim SG (2022). Ribozyme-processed guide RNA enhances virus-mediated plant genome editing. Biotechnol J 17, 2100189.
[50]	Pausch P, Al-Shayeb B, Bisom-Rapp E, Tsuchida CA, Li Z, Cress BF, Knott GJ, Jacobsen SE, Banfield JF, Doudna JA (2020). CRISPR-CasΦ from huge phages is a hypercompact genome editor. Science 369, 333-337. DOI PMID
[51]	Purkayastha A, Dasgupta I (2009). Virus-induced gene silencing: a versatile tool for discovery of gene functions in plants. Plant Physiol Biochem 47, 967-976.
[52]	Senthil-Kumar M, Mysore KS (2011). New dimensions for VIGS in plant functional genomics. Trends Plant Sci 16, 656-665. DOI PMID
[53]	Su YK, Qiu JR, Zhang H, Song ZQ, Wang JH (2019). Recent progress in evolutionary technology of CRISPR/Cas9 system for plant genome editing. Chin Bull Bot 54, 385-395. (in Chinese)
	苏钺凯, 邱镜仁, 张晗, 宋振巧, 王建华 (2019). CRISPR/ Cas9系统在植物基因组编辑中技术改进与创新的研究进展. 植物学报 54, 385-395. DOI
[54]	Tai YS, Bragg J, Meinhardt SW (2007). Functional characterization of toxa and molecular identification of its intracellular targeting protein in wheat. Am J Plant Physiol 2, 76-89.
[55]	Tamilselvan-Nattar-Amutha S, Hiekel S, Hartmann F, Lorenz J, Dabhi RV, Dreissig S, Hensel G, Kumlehn J, Heckmann S (2023). Barley stripe mosaic virus-mediated somatic and heritable gene editing in barley (Hordeum vulgare L.). Front Plant Sci 14, 1201446.
[56]	Uranga M, Aragonés V, Selma S, Vázquez-Vilar M, Orzáez D, Daròs JA (2021). Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector. Plant J 106, 555-565.
[57]	Wang D, Tai PWL, Gao GP (2019). Adeno-associated virus vector as a platform for gene therapy delivery. Nat Rev Drug Discov 18, 358-378. DOI PMID
[58]	Wang Q, Ma XN, Qian SS, Zhou X, Sun K, Chen XL, Zhou XP, Jackson AO, Li ZH (2015). Rescue of a plant negative-strand RNA virus from cloned cDNA: insights into enveloped plant virus movement and morphogenesis. PLoS Pathog 11, e1005223.
[59]	Wang W, Yu ZT, He F, Bai GH, Trick HN, Akhunova A, Akhunov E (2022). Multiplexed promoter and gene editing in wheat using a virus-based guide RNA delivery system. Plant Biotechnol J 20, 2332-2341. DOI PMID
[60]	Wu ZW, Zhang YF, Yu HP, Pan D, Wang YJ, Wang YN, Li F, Liu C, Nan H, Chen WZ, Ji QJ (2021). Programmed genome editing by a miniature CRISPR-Cas12f nuclease. Nat Chem Biol 17, 1132-1138. DOI PMID
[61]	Yin KQ, Han T, Liu G, Chen TY, Wang Y, Yu AYL, Liu YL (2015). A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing. Sci Rep 5, 14926. DOI PMID
[62]	Yip BH (2020). Recent advances in CRISPR/Cas9 delivery strategies. Biomolecules 10, 839.
[63]	Yuan C, Li C, Yan LJ, Jackson AO, Liu ZY, Han CG, Yu JL, Li DW (2011). A high throughput Barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots. PLoS One 6, e26468.
[64]	Zhan XQ, Lu YM, Zhu JK, Botella JR (2021). Genome editing for plant research and crop improvement. J Integr Plant Biol 63, 3-33. DOI
[65]	Zhang X, Kang LH, Zhang Q, Meng QQ, Pan YF, Yu ZM, Shi NN, Jackson S, Zhang XL, Wang HZ, Tor M, Hong YG (2020). An RNAi suppressor activates in planta virus- mediated gene editing. Funct Integr Genomics 20, 471-477.

病毒类型	病毒名称	受体植物	递送成分	非组织培养稳定遗传效率	参考文献
正链RNA病毒	烟草脆裂病毒	本氏烟草(Nicotiana benthamiana) (Cas9过表达)	sgRNAs	0.2%	Ali et al., 2015a
	烟草脆裂病毒和豌豆早枯病毒	本氏烟草和拟南芥(Arabidopsis thaliana) (Cas9过表达)	sgRNAs	N.A	Ali et al., 2015b, 2018
	烟草脆裂病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	65%-100%	Ellison et al., 2020
	烟草脆裂病毒	拟南芥(过表达SunTag系统)	sgRNAs和可移动 sgRNAs	5%-8%	Ghoshal et al., 2020
	烟草脆裂病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	39%-60%	Nagalakshmi et al., 2022
	烟草脆裂病毒	烟草(N. tabacum) (Cas9过表达)	sgRNAs	N.A	Oh et al., 2022
	烟草脆裂病毒和豌豆早枯病毒	烟草(Cas9过表达)	sgRNAs	N.A	Kim et al., 2023
	烟草花叶病毒	本氏烟草(Cas9过表达)	Cas9和sgRNAs	N.A	Cody et al., 2017
	烟草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Chiong et al., 2021
	番茄花叶病毒	本氏烟草(分开的SaCas9s部分过表达)	部分Split-SaCas9s和sgRNAs	N.A	Kaya et al., 2017
	苹果潜隐球形病毒	本氏烟草和大豆(Glycine max) (Cas9, Csy4过表达)	sgRNAs	N.A	Luo et al., 2021
	甜菜坏死黄脉病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Jiang et al., 2019
	大麦条纹花叶病毒	本氏烟草、小麦(Triticum aestivum)和玉米(Zea mays) (Cas9过表达)	sgRNAs	N.A	Hu et al., 2019
	大麦条纹花叶病毒	小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0-17%	Wang et al., 2022
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	12.9%-100%	Li et al., 2021
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0.5%-0.8%	Chen et al., 2022
	大麦条纹花叶病毒	本氏烟草和大麦(Hordeum vulgare) (Cas9过表达)	sgRNAs	100%	Tamilselvan-Nattar- Amutha et al., 2023
	马铃薯X病毒	本氏烟草	Cas9和sgRNAs	N.A	Ariga et al., 2020
	马铃薯X病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	22%	Uranga et al., 2021
	狗尾草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Zhang et al., 2020
	狗尾草花叶病毒	本氏烟草、玉米和狗尾草(Setaria viridis) (Cas9过表达)	sgRNAs	N.A	Mei et al., 2019
负链RNA 病毒	苦苣菜黄网弹状病毒	本氏烟草	Cas9和sgRNAs	N.A	Ma et al., 2020
	大麦黄条纹花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Gao et al., 2019
	番茄斑萎病毒	本氏烟草、番茄(Solanum lycopersicum)、辣椒(Capsicum annuum)、灯笼果(Physalis peruviana)和花生(Arachis hypogaea)	Cas9和sgRNAs, Cas12a和crRNA	N.A	Liu et al., 2023
DNA 病毒	甘蓝曲叶病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Yin et al., 2015
	棉皱叶病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	4.35%-8.79%	Lei et al., 2021
	棉皱叶病毒	棉花(Gossypium spp.) (Cas9过表达)	sgRNAs和可移动sgRNAs	N.A	Lei et al., 2022

病毒类型	病毒名称	受体植物	递送成分	非组织培养稳定遗传效率	参考文献
正链RNA病毒	烟草脆裂病毒	本氏烟草(Nicotiana benthamiana) (Cas9过表达)	sgRNAs	0.2%	Ali et al., 2015a
	烟草脆裂病毒和豌豆早枯病毒	本氏烟草和拟南芥(Arabidopsis thaliana) (Cas9过表达)	sgRNAs	N.A	Ali et al., 2015b, 2018
	烟草脆裂病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	65%-100%	Ellison et al., 2020
	烟草脆裂病毒	拟南芥(过表达SunTag系统)	sgRNAs和可移动 sgRNAs	5%-8%	Ghoshal et al., 2020
	烟草脆裂病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	39%-60%	Nagalakshmi et al., 2022
	烟草脆裂病毒	烟草(N. tabacum) (Cas9过表达)	sgRNAs	N.A	Oh et al., 2022
	烟草脆裂病毒和豌豆早枯病毒	烟草(Cas9过表达)	sgRNAs	N.A	Kim et al., 2023
	烟草花叶病毒	本氏烟草(Cas9过表达)	Cas9和sgRNAs	N.A	Cody et al., 2017
	烟草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Chiong et al., 2021
	番茄花叶病毒	本氏烟草(分开的SaCas9s部分过表达)	部分Split-SaCas9s和sgRNAs	N.A	Kaya et al., 2017
	苹果潜隐球形病毒	本氏烟草和大豆(Glycine max) (Cas9, Csy4过表达)	sgRNAs	N.A	Luo et al., 2021
	甜菜坏死黄脉病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Jiang et al., 2019
	大麦条纹花叶病毒	本氏烟草、小麦(Triticum aestivum)和玉米(Zea mays) (Cas9过表达)	sgRNAs	N.A	Hu et al., 2019
	大麦条纹花叶病毒	小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0-17%	Wang et al., 2022
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	12.9%-100%	Li et al., 2021
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0.5%-0.8%	Chen et al., 2022
	大麦条纹花叶病毒	本氏烟草和大麦(Hordeum vulgare) (Cas9过表达)	sgRNAs	100%	Tamilselvan-Nattar- Amutha et al., 2023
	马铃薯X病毒	本氏烟草	Cas9和sgRNAs	N.A	Ariga et al., 2020
	马铃薯X病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	22%	Uranga et al., 2021
	狗尾草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Zhang et al., 2020
	狗尾草花叶病毒	本氏烟草、玉米和狗尾草(Setaria viridis) (Cas9过表达)	sgRNAs	N.A	Mei et al., 2019
负链RNA 病毒	苦苣菜黄网弹状病毒	本氏烟草	Cas9和sgRNAs	N.A	Ma et al., 2020
	大麦黄条纹花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Gao et al., 2019
	番茄斑萎病毒	本氏烟草、番茄(Solanum lycopersicum)、辣椒(Capsicum annuum)、灯笼果(Physalis peruviana)和花生(Arachis hypogaea)	Cas9和sgRNAs, Cas12a和crRNA	N.A	Liu et al., 2023
DNA 病毒	甘蓝曲叶病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Yin et al., 2015
	棉皱叶病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	4.35%-8.79%	Lei et al., 2021
	棉皱叶病毒	棉花(Gossypium spp.) (Cas9过表达)	sgRNAs和可移动sgRNAs	N.A	Lei et al., 2022

病毒介导的植物基因组编辑技术研究进展

Advances in Virus-mediated Genome Editing Technology in Plants

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 65

相关文章 15

编辑推荐

Metrics

本文评价

[1]	陈龙郭柯勾晓华赵秀海马泓若. 祁连圆柏林群落组成及特征[J]. 植物生态学报, 2024, 48(预发表): 0-0.
[2]	王振宇黄志群. 亚热带27种木本植物叶片性状对植食作用的影响: 验证生长-防御权衡假说[J]. 植物生态学报, 2024, 48(预发表): 0-0.
[3]	牛云明贾国栋王欣刘子赫. 庐山不同海拔植被蒸腾水龄动态及用水策略[J]. 植物生态学报, 2024, 48(9): 0-0.
[4]	陈炫铮, 朱耀军, 高居娟, 刘一凡, 王荣, 方涛, 罗芳丽, 薛伟, 于飞海. 植物-土壤反馈时空变异研究进展[J]. 植物生态学报, 2024, 48(8): 955-966.
[5]	李邦泽, 张树仁. 中国莎草科最新物种名录和分类纲要[J]. 生物多样性, 2024, 32(7): 24106-.
[6]	郑博瀚, 陈鑫瑶, 倪健. 中国维管植物生长型和生活型数据集[J]. 生物多样性, 2024, 32(7): 23468-.
[7]	秦嘉晨, 王欢, 朱江, 王扬, 田晨, 白永飞, 杨培志, 郑淑霞. 基于种内与种间性状变异的放牧过滤作用及其尺度效应[J]. 植物生态学报, 2024, 48(7): 858-871.
[8]	胡宗刚. 抗战胜利后中美曾筹划合编《中国植物志》[J]. 生物多样性, 2024, 32(6): 24220-.
[9]	何花, 谭敦炎, 杨晓琛. 被子植物隐性雌雄异株性系统的多样性、系统演化及进化意义[J]. 生物多样性, 2024, 32(6): 24149-.
[10]	巴苏艳, 赵春艳, 刘媛, 方强. 通过虫体花粉识别构建植物‒传粉者网络: 人工模型与AI模型高度一致[J]. 生物多样性, 2024, 32(6): 24088-.
[11]	张强, 赵振宇, 李平华. 基因编辑技术在玉米中的研究与进展[J]. 植物学报, 2024, 59(6): 0-0.
[12]	胡心怡, 穆丹, 张惠雯, 秦华光, 白雅茹, 龙洪婕, 徐颖睿, 张雯菁, 邓洋, 周洲, 胡洁怡, 毕晨悦, 沈昭阳, 孙廷哲, 饶玉春. 基于高校植物学课程的生物科普教育探索[J]. 植物学报, 2024, 59(6): 0-0.
[13]	江康威, 张青青, 王亚菲, 李宏, 丁雨, 杨永强, 吐尔逊娜依•热依木. 放牧干扰下天山北坡中段植物功能群特征及其与土壤环境因子的关系[J]. 植物生态学报, 2024, 48(6): 701-718.
[14]	连佳丽, 陈婧, 杨雪琴, 赵莹, 罗叙, 韩翠, 赵雅欣, 李建平. 荒漠草原植物多样性和微生物多样性对降水变化的响应[J]. 生物多样性, 2024, 32(6): 24044-.
[15]	邓蓓, 王晓锋, 廖君. 环境胁迫影响三峡库区消落带草本和木本植物生理生态特征的meta分析[J]. 植物生态学报, 2024, 48(5): 623-637.