病毒介导的植物基因组编辑技术研究进展

doi:10.11983/CBB23046

植物学报 ›› 2024, Vol. 59 ›› Issue (3): 452-462.DOI: 10.11983/CBB23046

病毒介导的植物基因组编辑技术研究进展

胡丹玲, 孙永伟^*()

内蒙古大学生命科学学院, 呼和浩特 010000

收稿日期:2023-04-07 接受日期:2023-08-07 出版日期:2024-05-01 发布日期:2023-09-25
通讯作者: *孙永伟, 内蒙古大学生命科学学院副教授、硕士生导师。入选内蒙古自治区高等学校青年科技英才计划。先后主持国家自然科学基金项目3项, 内蒙古自然科学基金面上项目3项。主要从事牧草生物学及牧草基因组编辑体系建立相关研究。在Molecular Plant、Plant Biotechnology Journal和Frontiers in Plants Science等期刊发表学术论文10余篇, 授权国家发明专利10余项。E-mail: sunyongwei@imu.edu.cn
基金资助:
国家自然科学基金青年科学基金(31800206);国家自然科学基金地区科学基金(32160111);内蒙古自治区自然科学基金面上项目(2020MS03027)

Advances in Virus-mediated Genome Editing Technology in Plants

Danling Hu, Yongwei Sun^*()

School of Life Sciences, Inner Mongolia University, Hohhot 010000, China

Received:2023-04-07 Accepted:2023-08-07 Online:2024-05-01 Published:2023-09-25
Contact: *E-mail: sunyongwei@imu.edu.cn

摘要/Abstract

摘要： CRISPR/Cas作为一种新兴的靶向基因组编辑技术, 具有操作过程简便、编辑效率高和支持多靶点编辑等优势, 在植物遗传育种中应用前景广阔。然而对于一些尚未建立遗传转化体系和再生体系的植物, 基因组编辑技术的应用仍然受限。病毒介导的植物基因组编辑技术可不依赖遗传转化和再生等步骤, 即可快速获得无外源转基因成分的基因组编辑植物, 受到广泛关注。该文主要介绍了病毒介导的CRISPR/Cas植物基因组编辑技术的工作原理及优势, 系统总结了该技术在植物基因组编辑领域的应用现状, 并重点讨论了该技术体系存在的问题及挑战, 以期为深入开展这一领域研究提供参考。

关键词: 病毒, 植物, 基因组编辑, CRISPR/Cas

Abstract: As a new technology for targeted genome editing, clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (Cas) have the advantages of easy operation, high editing efficiency, and support for multi-target editing, thus showing wide application prospects in plant genetic breeding. However, the process in plants relies mainly on Agrobacterium- or particle bombardment-mediated genetic transformation, which is time-consuming as well as species- and varieties-dependent. Virus-mediated plant genome editing has attracted extensive atten- tion because of its no requirement of genetic transformation and plant regeneration. In this review, we introduce the working principle and advantages of virus-mediated CRISPR/Cas plant genome editing technology, systematically sum- marize the current application status of this technology in the field of plant genome editing, and focus on discussing the problems and challenges of this technology system, aiming to provide reference for further research and development in this field.

Key words: virus, plant, genome editing, CRISPR/Cas

胡丹玲, 孙永伟. 病毒介导的植物基因组编辑技术研究进展. 植物学报, 2024, 59(3): 452-462.

Danling Hu, Yongwei Sun. Advances in Virus-mediated Genome Editing Technology in Plants. Chinese Bulletin of Botany, 2024, 59(3): 452-462.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB23046

https://www.chinbullbotany.com/CN/Y2024/V59/I3/452

图/表 2

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病毒类型	病毒名称	受体植物	递送成分	非组织培养稳定遗传效率	参考文献
正链RNA病毒	烟草脆裂病毒	本氏烟草(Nicotiana benthamiana) (Cas9过表达)	sgRNAs	0.2%	Ali et al., 2015a
	烟草脆裂病毒和豌豆早枯病毒	本氏烟草和拟南芥(Arabidopsis thaliana) (Cas9过表达)	sgRNAs	N.A	Ali et al., 2015b, 2018
	烟草脆裂病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	65%-100%	Ellison et al., 2020
	烟草脆裂病毒	拟南芥(过表达SunTag系统)	sgRNAs和可移动 sgRNAs	5%-8%	Ghoshal et al., 2020
	烟草脆裂病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	39%-60%	Nagalakshmi et al., 2022
	烟草脆裂病毒	烟草(N. tabacum) (Cas9过表达)	sgRNAs	N.A	Oh et al., 2022
	烟草脆裂病毒和豌豆早枯病毒	烟草(Cas9过表达)	sgRNAs	N.A	Kim et al., 2023
	烟草花叶病毒	本氏烟草(Cas9过表达)	Cas9和sgRNAs	N.A	Cody et al., 2017
	烟草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Chiong et al., 2021
	番茄花叶病毒	本氏烟草(分开的SaCas9s部分过表达)	部分Split-SaCas9s和sgRNAs	N.A	Kaya et al., 2017
	苹果潜隐球形病毒	本氏烟草和大豆(Glycine max) (Cas9, Csy4过表达)	sgRNAs	N.A	Luo et al., 2021
	甜菜坏死黄脉病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Jiang et al., 2019
	大麦条纹花叶病毒	本氏烟草、小麦(Triticum aestivum)和玉米(Zea mays) (Cas9过表达)	sgRNAs	N.A	Hu et al., 2019
	大麦条纹花叶病毒	小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0-17%	Wang et al., 2022
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	12.9%-100%	Li et al., 2021
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0.5%-0.8%	Chen et al., 2022
	大麦条纹花叶病毒	本氏烟草和大麦(Hordeum vulgare) (Cas9过表达)	sgRNAs	100%	Tamilselvan-Nattar- Amutha et al., 2023
	马铃薯X病毒	本氏烟草	Cas9和sgRNAs	N.A	Ariga et al., 2020
	马铃薯X病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	22%	Uranga et al., 2021
	狗尾草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Zhang et al., 2020
	狗尾草花叶病毒	本氏烟草、玉米和狗尾草(Setaria viridis) (Cas9过表达)	sgRNAs	N.A	Mei et al., 2019
负链RNA 病毒	苦苣菜黄网弹状病毒	本氏烟草	Cas9和sgRNAs	N.A	Ma et al., 2020
	大麦黄条纹花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Gao et al., 2019
	番茄斑萎病毒	本氏烟草、番茄(Solanum lycopersicum)、辣椒(Capsicum annuum)、灯笼果(Physalis peruviana)和花生(Arachis hypogaea)	Cas9和sgRNAs, Cas12a和crRNA	N.A	Liu et al., 2023
DNA 病毒	甘蓝曲叶病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Yin et al., 2015
	棉皱叶病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	4.35%-8.79%	Lei et al., 2021
	棉皱叶病毒	棉花(Gossypium spp.) (Cas9过表达)	sgRNAs和可移动sgRNAs	N.A	Lei et al., 2022

病毒类型	病毒名称	受体植物	递送成分	非组织培养稳定遗传效率	参考文献
正链RNA病毒	烟草脆裂病毒	本氏烟草(Nicotiana benthamiana) (Cas9过表达)	sgRNAs	0.2%	Ali et al., 2015a
	烟草脆裂病毒和豌豆早枯病毒	本氏烟草和拟南芥(Arabidopsis thaliana) (Cas9过表达)	sgRNAs	N.A	Ali et al., 2015b, 2018
	烟草脆裂病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	65%-100%	Ellison et al., 2020
	烟草脆裂病毒	拟南芥(过表达SunTag系统)	sgRNAs和可移动 sgRNAs	5%-8%	Ghoshal et al., 2020
	烟草脆裂病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	39%-60%	Nagalakshmi et al., 2022
	烟草脆裂病毒	烟草(N. tabacum) (Cas9过表达)	sgRNAs	N.A	Oh et al., 2022
	烟草脆裂病毒和豌豆早枯病毒	烟草(Cas9过表达)	sgRNAs	N.A	Kim et al., 2023
	烟草花叶病毒	本氏烟草(Cas9过表达)	Cas9和sgRNAs	N.A	Cody et al., 2017
	烟草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Chiong et al., 2021
	番茄花叶病毒	本氏烟草(分开的SaCas9s部分过表达)	部分Split-SaCas9s和sgRNAs	N.A	Kaya et al., 2017
	苹果潜隐球形病毒	本氏烟草和大豆(Glycine max) (Cas9, Csy4过表达)	sgRNAs	N.A	Luo et al., 2021
	甜菜坏死黄脉病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Jiang et al., 2019
	大麦条纹花叶病毒	本氏烟草、小麦(Triticum aestivum)和玉米(Zea mays) (Cas9过表达)	sgRNAs	N.A	Hu et al., 2019
	大麦条纹花叶病毒	小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0-17%	Wang et al., 2022
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	12.9%-100%	Li et al., 2021
	大麦条纹花叶病毒	本氏烟草和小麦(Cas9过表达)	sgRNAs和可移动sgRNAs	0.5%-0.8%	Chen et al., 2022
	大麦条纹花叶病毒	本氏烟草和大麦(Hordeum vulgare) (Cas9过表达)	sgRNAs	100%	Tamilselvan-Nattar- Amutha et al., 2023
	马铃薯X病毒	本氏烟草	Cas9和sgRNAs	N.A	Ariga et al., 2020
	马铃薯X病毒	本氏烟草(Cas9过表达)	sgRNAs和可移动sgRNAs	22%	Uranga et al., 2021
	狗尾草花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Zhang et al., 2020
	狗尾草花叶病毒	本氏烟草、玉米和狗尾草(Setaria viridis) (Cas9过表达)	sgRNAs	N.A	Mei et al., 2019
负链RNA 病毒	苦苣菜黄网弹状病毒	本氏烟草	Cas9和sgRNAs	N.A	Ma et al., 2020
	大麦黄条纹花叶病毒	本氏烟草	Cas9和sgRNAs	N.A	Gao et al., 2019
	番茄斑萎病毒	本氏烟草、番茄(Solanum lycopersicum)、辣椒(Capsicum annuum)、灯笼果(Physalis peruviana)和花生(Arachis hypogaea)	Cas9和sgRNAs, Cas12a和crRNA	N.A	Liu et al., 2023
DNA 病毒	甘蓝曲叶病毒	本氏烟草(Cas9过表达)	sgRNAs	N.A	Yin et al., 2015
	棉皱叶病毒	拟南芥(Cas9过表达)	sgRNAs和可移动sgRNAs	4.35%-8.79%	Lei et al., 2021
	棉皱叶病毒	棉花(Gossypium spp.) (Cas9过表达)	sgRNAs和可移动sgRNAs	N.A	Lei et al., 2022

病毒介导的植物基因组编辑技术研究进展

Advances in Virus-mediated Genome Editing Technology in Plants

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