植物学报 ›› 2024, Vol. 59 ›› Issue (3): 0-0.DOI: 10.11983/CBB23046

• 专题论坛 •    下一篇

病毒介导的植物基因组编辑技术研究进展

胡丹玲, 孙永伟   

  1. 内蒙古大学生命科学学院, 呼和浩特 010000

  • 收稿日期:2023-04-09 修回日期:2023-07-18 出版日期:2024-05-01 发布日期:2023-09-25
  • 通讯作者: 孙永伟
  • 基金资助:
    国家自然科学基金青年科学基金(No.31800206)、国家自然科学基金地区科学基金(No.32160111)和内蒙古自治区自然科学基金面上项目(No.2020MS03027)

Advances in Virus-mediated Genome Editing in Plants

Danling Hu, Yongwei Sun   

  1. School of Life Sciences, Inner Mongolia University, Hohhot 010000, China

  • Received:2023-04-09 Revised:2023-07-18 Online:2024-05-01 Published:2023-09-25
  • Contact: Yongwei Sun

摘要: 作为一种新兴的靶向基因组编辑技术, 具有操作过程简便、编辑效率高和支持多靶点编辑等优势, 在植物遗传育种中展现了广阔的应用前景。然而对于一些遗传转化体系和再生体系尚未成熟的植物, 基因组编辑技术的应用仍然受限。病毒介导的植物基因组编辑技术可以不依赖于遗传转化及再生等步骤, 即可快速获得无转基因成份的基因组编辑植物而受到广泛关注。该文主要介绍了病毒介导的CRISPR/Cas植物基因组编辑技术的工作原理及优势, 系统的总结了该技术在植物基因组编辑领域中的应用现状, 并重点讨论了该技术体系存在的问题及挑战, 以期为深入开展这一领域的研究提供参考。

关键词: 病毒, 植物, 基因组编辑, CRIPSR/Cas

Abstract: As a new technology for targeted genome editing, clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (Cas) have the advantages of easy operation, high editing efficiency, and support for multi-target editing, thus shown wide application prospects in plant genetic breeding. However, the process in plants relies mainly on Agrobacterium or particle bombardment-mediated genetictrans formation, which is time-consuming and species- and varieties-dependent. Virus-mediated plant genome editing has attracted extensive attention because of no need of genetic transformation and plant regeneration.In this review, we introduces the working principle and advantages of virus-mediated CRISPR/Cas plant genome editing technology, systematically summarizes the current application status of this technology in the field of plant genome editing, and focuses on discussing the problems and challenges of this technology system, aiming to provide reference for further research in this field.

Key words: virus, plant, genome editing, CRIPSR/Cas