Abstract: Using the biological primary databases at the National Center for Biotechnology Information (NCBI), we constructed a secondary database of phytohormone-related nucleotides and proteins. The secondary database included data on genes, proteins and literature. Data were extracted from the NCBI databases by specially programmed software and stored in XML files, then parsed and loaded into the secondary database. The secondary database could be used for some preliminary applications such as data retrieval, statistical analysis, local web-based BLAST alignment, automatic sequence assembly and protein structure prediction after integrating some bioinformatics tools. This secondary database provides a highly specific phytohormonal data source and a supplementary bioinformatics tool for research into phytohormonal actions and their molecular mechanisms.