Abstract: Lipids provide both the structural basis of the cell membrane and fuel for metabolism. In plants, increasing evidence has demonstrated roles for lipids in cellular processes. As one of the most important branches of metabolomics, lipidomics may be a comprehensive strategy to study the cellular lipidomes and their biological roles in terms of protein expression and gene regulation involved in lipid metabolism and function. Progress in lipidomics has been driven by development in analytical methods and approaches. In recent years, with the increasing attention to and interest in lipidomics, great progress has been made in analytical strategies for lipidomics. Some advanced strategies to analyze lipid composition in animals have been successfully extended to plants. This review gives an overview of the current strategies in lipid profiling, to promote the development of lipidomics, especially plant lipidomics.