Abstract: Suspension culture of Acer ginnala was established with cotyledonsus ed as explants. WPM was used for callus induction and callus proliferation. In suspension culture, dry weight increased to 11.6 and gallic acid content to 1.518%. The effects of the media NT, IS, WPM, B5 and MS on growth and gallic acid content of A. ginnala in callus suspension culture were compared. WPM was best for growth of suspension cells and accumulation of gallic acid. The effect increased with the levels of major elements, and a three-fold increase in level of major elements gave the best result. Sucrose at 10 g·L-1 was suitable for the accumulation of gallic acid, and 30 g·L-1 was optimal for cell growth and production of gallic acid.