Abstract: In this research, we used a recombinant inbred line (RIL) population of 111 lines derived from a cross of Reyan2 (Oryza sativa subsp. japonica ‘Reyan2’)/ Milyang23 (Oryza sativa subsp. indica ‘Milyang23’) by the single-seed descent method to detect quantitative trait loci (QTL) conferring resistance to rice stripe virus (RSV). Reactions of the two parents and the 111 RILs to RSV were investigated by natural methods of viral infection in the field and scored by disease index . QTL for resistance to RSV was analyzed by QTL Cartographer software. Chromosomes 2 and 11 each revealed one QTL controlling RSV resistance. The phenotypic variance explained by the QTL on chromosome 11 was 19.58%, so the locus is a major effective one controlling resistance to RSV and should be useful in breeding rice for resistance to RSV in a marker-assisted selection program.