Abstract: To investigate the effect of the quantity of primer combinations on identifying genetic relationships among bamboo species accurately , we analyzed the diversity of 26 bamboo species with amplified fragment length polymorphism(AFLP). We selected 10 pairs of AFLP primer, and the 10 primer combinations of AFLP markers were analyzed by Nei's genetic distance/unw eighted pair group method analysis (UPGMA). Each pair of primers resulted in a group designation, and the relations among the 26 species were confirmed consistently with an increased number of random combinations of AFLP markers used as a clustering statistic. For study of phylogenetics, a sufficient quantity of primer combinations can reveal exact clustering relations. The method of accumulating random combinations of AFLP primers and clustering analysis was applied, and the least number of combinations for testing breeding was confirmed by clustering relations.